Draft Genome Sequence of <I>Cercospora canescens</I>: A Leaf Spot Causing Pathogen

Abstract

Cercospora canescens (Ellis and Martin ) is a hemibiotrophic pathogen causing leaf spot disease on mungbean (Vigna radiata L). Genome sequence (~33.97 Mb) assembled in 8239 contigs with 10627 protein coding genes. A total of 2842 proteins were identified as homologous of 223 predicted and 7562 putative uncharacterized involved in biological processes, molecular functions. The identified proteins are mainly involved in infection process used to compromise nutrients or destroy host tissues, the gycosidases, transposases and cytochrome P450s genes codes to signal transduction, cell wall breakdown, transporters, host stomata perception, adhesion, polyketide synthase and cercosporin. A total of 528 simple sequence repeats were also identified from the genome sequence assembly of C. canescens. This study provides insights into pathogenic mechanism and a better understanding of virulence differentiation of C. canescens. It will also help in identification of similarity and differences in regions among the genomes of different species of Cercospora.

Cryptic diversity, multilocus phylogeny, and pathogenicity of cercosporoid fungi associated with common bean and cowpea

“…It is worth noting that C . canescens , one of the most widespread and significant causal agents of CLS in legumes (Chand et al, 2015; Farr & Rossman, 2021), was not detected in this study, although it was previously linked to CLS symptoms of cowpea and common bean in Iran based on morphological data (Ershad, 2009). These results underline the unreliability of old records of Cercospora species based on morphological data and the need to explore true geographical boundaries of different species based on molecular studies of freshly collected specimens.…”

“…Cercospora leaf spot (CLS) disease is among the most important diseases in bean production in the world, and can cause yield losses of up to 40% (Fery et al, 1977; Schneider et al, 1976). Cercospora canescens , Pseudocercospora cruenta , and P. griseola are known as common causal agents of CLS in common bean and cowpea crops (Chand et al, 2015; Crous et al, 2006; Duangsong et al, 2018; Serrato‐Diaz et al, 2020); however, other species of these genera including C . albida , C .…”

Delineating Genotype × Environment interactions towards durable resistance in mungbean against Cercospora leaf spot (Cercospora canescens) using GGE biplot

“…The disease spread is very rapid during the pod‐filling stage. In susceptible varieties, infection inflates rapidly resulting in premature defoliation and reduction in size of pods and seeds, with significant yield losses (46%–61%) (Chand et al, ; Sompong, Somta, Sorjjapinum, & Srinives, ). The fungus is ubiquitous in nature but its severity depends on the dynamic weather conditions resulting in sporadic outbreaks (Kimber & Paull, ).…”

“…Presence of host and pathogenic variability (Chand, Singh, Chhattarpal, & Kumar, ) seeks immediate attention of identification of durable resistance sources against CLS in mungbean followed by utilization of these sources as donors in resistance breeding pogramme for managing the issue in comprehensive manner. Unfortunately, progress in breeding CLS resistant cultivar has been limited due to complicacy in screening process, wider host range and intricacy due to species complexity of the genus Cercospora (Barbetti, ; Chand et al, ; Chankaew et al, ).…”

Yield performance of eight mungbean (Phaseolus radiatus) genotypes in two locations in Manggarai District, East Nusa Tenggara, Indonesia

“…In addition to the above mentioned constraints in mungbean production and productivity, water shortage and foliar diseases such as Cercosporalef spot, rust, powdery mildew, scab, and viruses are other factors that also contributed significantly to the low mungbean production (Chand et al 2015;Gnanaraj et al 2015;Kumar et al 2017;Parihar et al 2017). The use of superior varieties is, therefore, considered the most effective and efficient way of overcoming the above-mentioned mungbean production constraints.…”