Draft Genome Sequence of Kingella negevensis SW7208426, the First European Strain of
            <i>K. negevensis</i>
            Isolated from a Healthy Child in Switzerland

Houmami, Nawal El; Schrenzel, Jacques; Yagupsky, Pablo; Robert, Caroline; Ceroni, Dimitri; Raoult, Didier; Fournier, Pierre‐Edouard

doi:10.1128/genomea.00571-17

Cited by 7 publications

(8 citation statements)

References 10 publications

(13 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A 705-bp mobile element (ISKne1), located 1,121 bp downstream of the tolC gene and flanked by two nearly perfect terminal inverted repeats of 29 bp, was identified within K. negevensis strains Sch538 T and SW7204826. Spread in multiple copies within K. negevensis genomes and sometimes bordered by an 8-bp direct repeat (TAGCTGTT), ISKne1 was present in DNA regions with GC contents ranging from 30.0 to 36.0%, contrasting with the 45.5% GC content of the K. negevensis genome (6,25). The presumed ISKne1 protein contains 234 amino acids, including D106, D165, E191, and R198, which are catalytic amino acid residues highly conserved in IS1 transposases with a DDER motif (26) (Fig.…”

Section: Resultsmentioning

confidence: 99%

Molecular Tests That Target the RTX Locus Do Not Distinguish between Kingella kingae and the Recently Described Kingella negevensis Species

et al. 2017

Self Cite

View full text Add to dashboard Cite

is an important invasive pathogen in early childhood. The organism elaborates an RTX toxin presumably restricted to this species. Consequently, real-time quantitative PCR (qPCR) assays targeting the RTX locus have been developed in recent years and are gaining increasing use for the molecular diagnosis of infections. However, the present study shows that, a species newly identified in young children, harbors an identical RTX locus, raising the question of whether can be misidentified as by clinical microbiology laboratories. comparison of sp. RTX and genes and studies provided evidence that targeting the and genes could not differentiate between strains of and, whereas targeting the gene could. This prompted the design of a highly specific and sensitive qPCR assay targeting (). Ninety-nine culture-negative osteoarticular specimens from 99 children younger than 4 years of age were tested with a conventional 16S rRNA gene-based broad-range PCR assay and -specific, -specific (), and qPCR assays. Forty-two specimens were positive, including 41 that were also positive and 1 (the remaining one) that was positive. Thus, this study discloses an invasive infection caused by in humans and demonstrates that targeting the RTX locus cannot be used for the formal diagnosis of infections. These findings stress the need for further studies on the epidemiology of asymptomatic carriage and invasive infections caused by in humans.

show abstract

Section: Resultsmentioning

confidence: 99%

Molecular Tests That Target the RTX Locus Do Not Distinguish between Kingella kingae and the Recently Described Kingella negevensis Species

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, the described assay did cross-react with the newly described species K. negevensis sp. nov., 24,38 as expected based on the performance of other assays targeting the RTX gene locus. 39 rtxB has been used as a PCR target by other investigators, 14 as has rtxA.…”

Section: Discussionsupporting

confidence: 71%

“…39 Recent reports also suggest that K. negevensis sp. nov., like K. kingae, can colonize the oropharynx of children; 24,38 given this and that it has the same RTX gene locus, 39,40 it is possible that K. negevensis sp. nov. may be a cause of OAI in children, but further studies on this subject are required.…”

Section: Discussionmentioning

confidence: 99%

Real-Time PCR Assay for Detection of Kingella kingae in Children

Madigan

Cunningham

Ramanan

et al. 2018

J Pediatr Infect Dis

View full text Add to dashboard Cite

Background Kingella kingae is a known cause of osteoarticular infections in children younger than 4 years of age, but it is not always recoverable in culture. Molecular methods are increasingly used for diagnosis. Methods To facilitate diagnosis of K. kingae septic arthritis, we developed a real-time polymerase chain reaction (PCR) assay for the detection of K. kingae that targets the repeat-in-toxin gene (rtxB). Results We present three pediatric patients with K. kingae septic arthritis at our institution who were diagnosed using the real-time PCR assay. All underwent arthrotomy with irrigation and debridement and were symptom-free after 3 weeks of therapy with β-lactam antibiotics. Cultures of synovial fluid or tissue grew K. kingae in two of three; K. kingae real-time PCR was positive in all three patients. In addition, 11 cases of K. kingae osteoarticular infection were diagnosed through Mayo Medical Laboratories using this assay. The limit of detection of the real-time PCR assay was 73.7 colony-forming unit (CFU)/µL for tissue and 1.3 CFU/µL for synovial fluid. Conclusions PCR-based detection methods are faster and more sensitive than conventional culture-based methods for the diagnosis of K. kingae osteoarticular infections in children.

show abstract

“…Kingella negevensis is a novel Kingella species that has been isolated primarily from the oropharynx of healthy children in Israel and Switzerland [20,22]. Closely related to K. kingae phenotypically and genetically, K. negevensis not only shares the same colonization niche than K. kingae, but also harbors an identical RTX locus within its genome, suggesting that the organism may also produce a ß-hemolytic and cytotoxic RTX toxin [20].…”

Section: Kingella Nevegensis; a Novel Paediatric Pathogen?mentioning

confidence: 99%

Kingella kingae: from oropharyngeal carriage to paediatric osteoarticular infections

Valaikaitė

Houmami

Spyropoulou

et al. 2018

Expert Review of Anti-infective Therapy

Self Cite

View full text Add to dashboard Cite

Draft Genome Sequence of Kingella negevensis SW7208426, the First European Strain of K. negevensis Isolated from a Healthy Child in Switzerland

Abstract: We report here the draft genome of Kingella negevensis strain SW7208426, isolated from the oropharynx of a healthy 6-year-old boy in Geneva, Switzerland. To our knowledge, this is the first genome report of the newly described K. negevensis species from Europe.

Cited by 7 publications

References 10 publications

Molecular Tests That Target the RTX Locus Do Not Distinguish between Kingella kingae and the Recently Described Kingella negevensis Species

Molecular Tests That Target the RTX Locus Do Not Distinguish between Kingella kingae and the Recently Described Kingella negevensis Species

Real-Time PCR Assay for Detection of Kingella kingae in Children

Kingella kingae: from oropharyngeal carriage to paediatric osteoarticular infections

Contact Info

Product

Resources

About