Draft Genome Sequence of Nonomuraea sp. Strain C10, a Producer of Brartemicin, Isolated from a Mud Dauber Wasp Nest in Nepal

Abstract

Nonomuraea sp. strain C10 produces the cytotoxic natural product brartemicin. Here, we report its draft genome sequence to get insight into brartemicin biosynthesis and to enable genome mining for novel secondary metabolites.

Draft Genome Sequence of the Sattazolin-Producing Strain Pseudonocardia sp. C8, Isolated from a Mud Dauber Wasp Nest in Nepal

“…As part of our ongoing collaborative effort to investigate bacteria from underexplored resources ( 1 ), we isolated and characterized another mud dauber wasp nest-associated actinobacterium. In chemical analyses, it became apparent that strain C8 produces the antiviral compound sattazolin ( 2 – 4 ).…”

“…The isolation of the strain was conducted as published previously ( 1 ). For genomic DNA isolation, strain C8 was grown in seed medium ( 1 ) for 8 days at 30°C on a rotary shaker (160 rpm).…”

“…The isolation of the strain was conducted as published previously ( 1 ). For genomic DNA isolation, strain C8 was grown in seed medium ( 1 ) for 8 days at 30°C on a rotary shaker (160 rpm). For genomic DNA isolation, a Quick-DNA fungal/bacterial DNA miniprep kit (Zymo Research, Irvine, CA, USA) was used according to the manufacturer’s protocol except that the vortex-mixing step was reduced from 15 min to 5 min and was conducted at maximum speed.…”