Draft Genome Sequence of
            <i>Frankia</i>
            sp. Strain Thr, a Nitrogen-Fixing Actinobacterium Isolated from the Root Nodules of
            <i>Casuarina cunninghamiana</i>
            Grown in Egypt

Hurst, Sheldon G.; Oshone, Rediet; Ghodhbane‐Gtari, Faten; Morris, Krystalynne; Abebe-Akele, Feseha; Thomas, W. Kelley; Ktari, Amir; Salem, Karima; Mansour, Samira R.; Gtari, Maher; Tisa, Louis S.

doi:10.1128/genomea.00493-14

Cited by 33 publications

(5 citation statements)

References 21 publications

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“…As previously expected (Dobritsa 1998), strains world widely isolated from Allocasuarina and Casuarina species (Australia; KB5 (Rosbrook et al 1989), India; Cg70.9, Senegal; CeD (Diem et al 1982), Brazil; BR, Tunisia; BMG5.23 (Ghodhbane- Gtari et al 2010), and several strains from Egypt; CgIM4, CgIS1, CcI156 and CcI6 (Mansour and Moussa 2005), Allo2 and Thr (Girgis and Schwencke 1993) fall into Frankia casuarinae species based on dDDH and ANI values that range from 94.6 to 95.7% and 99.3-99.9% respectively. The genome size of these strains ranges from 4.9 to 5.6 with G+C content of 69.3 to70.1 mol% (Ghodbane- Hurst et al 2014;Mansour et al 2014;D'Angelo et al 2016;Ngom et al 2016;Pesce et al 2017).…”

Section: Myricaceaementioning

confidence: 99%

An update on the taxonomy of the genus Frankia Brunchorst, 1886, 174AL

Gtari

Nouioui

Sarkar

et al. 2018

Antonie van Leeuwenhoek

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Since the recognition of the name Frankia in the Approved Lists of bacterial names (1980), few amendments have been given to the genus description. Successive editions of Bergey's Manual of Systematics of Archaea and Bacteria have broadly conflicting suprageneric treatments of the genus without any advances for subgeneric classification. This review focuses on recent results from taxongenomics and phenoarray approaches to the positioning and the structuring of the genus Frankia. Based on phylogenomic analyses, Frankia should be considered the single member of the family Frankiaceae within the monophyletic order, Frankiales. A polyphasic strategy incorporating genome to genome data and omniLog phenoarrays, together with classical approaches, has allowed the designation and an amended description of a type strain of the type species Frankia alni, and the recognition of at least 10 novel species covering symbiotic and non symbiotic taxa within the genus. Genome to phenome data will be shortly incorporated in the scheme for proposing novel species including those recalcitrant to isolation in axenic culture.

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Section: Myricaceaementioning

confidence: 99%

An update on the taxonomy of the genus Frankia Brunchorst, 1886, 174AL

Gtari

Nouioui

Sarkar

et al. 2018

Antonie van Leeuwenhoek

Self Cite

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“…(2016) for Allo2, Ghodhbane-Gtari et al. (2014) for BMG5.23, Hurst et al (2014) for Thr, Ngom et al. (2016) for CeD, Wall et al.…”

Section: Introductionmentioning

confidence: 99%

Frankia-Enriched Metagenomes from the Earliest Diverging Symbiotic Frankia Cluster: They Come in Teams

Nguyen

Wibberg

Vigil-Stenman

et al. 2019

Genome Biology and Evolution

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Frankia strains induce the formation of nitrogen-fixing nodules on roots of actinorhizal plants. Phylogenetically, Frankia strains can be grouped in four clusters. The earliest divergent cluster, cluster-2, has a particularly wide host range. The analysis of cluster-2 strains has been hampered by the fact that with two exceptions, they could never be cultured. In this study, 12 Frankia-enriched metagenomes of Frankia cluster-2 strains or strain assemblages were sequenced based on seven inoculum sources. Sequences obtained via DNA isolated from whole nodules were compared with those of DNA isolated from fractionated preparations enhanced in the Frankia symbiotic structures. The results show that cluster-2 inocula represent groups of strains, and that strains not represented in symbiotic structures, that is, unable to perform symbiotic nitrogen fixation, may still be able to colonize nodules. Transposase gene abundance was compared in the different Frankia-enriched metagenomes with the result that North American strains contain more transposase genes than Eurasian strains. An analysis of the evolution and distribution of the host plants indicated that bursts of transposition may have coincided with niche competition with other cluster-2 Frankia strains. The first genome of an inoculum from the Southern Hemisphere, obtained from nodules of Coriaria papuana in Papua New Guinea, represents a novel species, postulated as Candidatus Frankia meridionalis. All Frankia-enriched metagenomes obtained in this study contained homologs of the canonical nod genes nodABC; the North American genomes also contained the sulfotransferase gene nodH, while the genome from the Southern Hemisphere only contained nodC and a truncated copy of nodB.

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“…Actinobacteria represent 20 to 30% of rhizospheric communities and include Gram-positive, high-GC-content (>50%), soil-dwelling organisms that play a significant role in nitrogen fixation ( 1 ). Frankia strains are endosymbionts in dicotyledonous plants that can enhance plant growth, solubilize phosphorus, and liberate indole-3-acetic acid (IAA) and siderophores ( 2 – 5 ). Non- Frankia actinobacterial species, including Micromonospora , Streptomyces , and Actinoplanes species, can also act as helper bacteria that stimulate plant growth.…”

Section: Announcementmentioning

confidence: 99%