Draft Genome Sequence of
            <i>Campylobacter jejuni</i>
            Bf, an Atypical Strain Able To Grow under Aerobiosis

Bronnec, Vicky; Haddad, Nabila; Cruveiller, Stéphane; Hernould, Michel; Tresse, Odile; Zagorec, Monique

doi:10.1128/genomea.00120-16

Cited by 7 publications

(10 citation statements)

References 9 publications

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“…The analysis of the draft genome of C. jejuni Bf did not reveal any clear gene acquisition or deletion which could explain its ability to grow under aerobiosis ( Bronnec et al, 2016 ). In the present study we completed the genome sequence and a deeper analysis of the gene repertoire of this strain was conducted.…”

Section: Resultsmentioning

confidence: 98%

“…To complete the draft genome sequence of C. jejuni Bf ( Bronnec et al, 2016 ), PCR amplifications were performed on regions presenting uncertainties and for gap-filling purpose on contig extremities with primers designed in the flanking regions of each gap and PCR products were sequenced (Biofidal, France). As genome comparison showed that C. jejuni Bf was closer to other genomes than that of the reference genome of C. jejuni NCTC 11168 a new mapping was performed on the closest complete genome available ( C. jejuni ATCC 32488 SRZ049709).…”

Section: Methodsmentioning

confidence: 99%

“…Finally, genome comparison was also performed in order to detect genetic elements putatively involved in the phenotype of this strain. For that purpose, the draft genome ( Bronnec et al, 2016 ) was completed and the gene and metabolic repertoires of C. jejuni Bf were compared to those of other complete or draft genomes.…”

Section: Introductionmentioning

confidence: 99%

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Adhesion, Biofilm Formation, and Genomic Features of Campylobacter jejuni Bf, an Atypical Strain Able to Grow under Aerobic Conditions

et al. 2016

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Campylobacter jejuni is the leading cause of bacterial enteritis in Europe. Human campylobacteriosis cases are frequently associated to the consumption of contaminated poultry meat. To survive under environmental conditions encountered along the food chain, i.e., from poultry digestive tract its natural reservoir to the consumer’s plate, this pathogen has developed adaptation mechanisms. Among those, biofilm lifestyle has been suggested as a strategy to survive in the food environment and under atmospheric conditions. Recently, the clinical isolate C. jejuni Bf has been shown to survive and grow under aerobic conditions, a property that may help this strain to better survive along the food chain. The aim of this study was to evaluate the adhesion capacity of C. jejuni Bf and its ability to develop a biofilm. C. jejuni Bf can adhere to abiotic surfaces and to human epithelial cells, and can develop biofilm under both microaerobiosis and aerobiosis. These two conditions have no influence on this strain, unlike results obtained with the reference strain C. jejuni 81-176, which harbors only planktonic cells under aerobic conditions. Compared to 81-176, the biofilm of C. jejuni Bf is more homogenous and cell motility at the bottom of biofilm was not modified whatever the atmosphere used. C. jejuni Bf whole genome sequence did not reveal any gene unique to this strain, suggesting that its unusual property does not result from acquisition of new genetic material. Nevertheless some genetic particularities seem to be shared only between Bf and few others strains. Among the main features of C. jejuni Bf genome we noticed (i) a complete type VI secretion system important in pathogenicity and environmental adaptation; (ii) a mutation in the oorD gene involved in oxygen metabolism; and (iii) the presence of an uncommon insertion of a 72 amino acid coding sequence upstream from dnaK, which is involved in stress resistance. Therefore, the atypical behavior of this strain under aerobic atmosphere may result from the combination of insertions and mutations. In addition, the comparison of mRNA transcript levels of several genes targeted through genome analysis suggests the modification of regulatory processes in this strain.

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Section: Resultsmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

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Adhesion, Biofilm Formation, and Genomic Features of Campylobacter jejuni Bf, an Atypical Strain Able to Grow under Aerobic Conditions

et al. 2016

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“…It is also well-known that Campylobacter spp. exhibit high genetic and phenotypic variability and flexibility (Gripp et al, 2011 ; Rodrigues et al, 2015 ; Bronnec et al, 2016 ), and, as a consequence, are not equally virulent and probably not equally sensitive to probiotic actions (Wine et al, 2009 ).…”

Section: In Vitro Screening For Anti- Campylobactementioning

confidence: 99%

Recent Advances in Screening of Anti-Campylobacter Activity in Probiotics for Use in Poultry

et al. 2016

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Campylobacteriosis is the most common cause of bacterial gastroenteritis worldwide. Campylobacter species involved in this infection usually include the thermotolerant species Campylobacter jejuni. The major reservoir for C. jejuni leading to human infections is commercial broiler chickens. Poultry flocks are frequently colonized by C. jejuni without any apparent symptoms. Risk assessment analyses have identified the handling and consumption of poultry meat as one of the most important sources of human campylobacteriosis, so elimination of Campylobacter in the poultry reservoir is a crucial step in the control of this foodborne infection. To date, the use of probiotics has demonstrated promising results to reduce Campylobacter colonization. This review provides recent insights into methods used for probiotic screening to reduce the prevalence and colonization of Campylobacter at the farm level. Different eukaryotic epithelial cell lines are employed to screen probiotics with an anti-Campylobacter activity and yield useful information about the inhibition mechanism involved. These in vitro virulence models involve only human intestinal or cervical cell lines whereas the use of avian cell lines could be a preliminary step to investigate mechanisms of C. jejuni colonization in poultry in the presence of probiotics. In addition, in vivo trials to evaluate the effect of probiotics on Campylobacter colonization are conducted, taking into account the complexity introduced by the host, the feed, and the microbiota. However, the heterogeneity of the protocols used and the short time duration of the experiments lead to results that are difficult to compare and draw conclusions at the slaughter-age of broilers. Nevertheless, the combined approach using complementary in vitro and in vivo tools (cell cultures and animal experiments) leads to a better characterization of probiotic strains and could be employed to assess reduced Campylobacter spp. colonization in chickens if some parameters are optimized.

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“…This was correlated to a lower susceptibility to compounds generating superoxide and peroxide stresses (Rodrigues et al, 2015 ). The first analyses of the genome of Bf did not reveal any deletion/insertion, SNPs, or organization of the genes already described to be involved in the sub-system of oxygen detoxification in other C. jejuni strains (Bronnec et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Comparison of Proteomics Profiles of Campylobacter jejuni Strain Bf under Microaerobic and Aerobic Conditions

et al. 2016

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Campylobacter jejuni accounts for one of the leading causes of foodborne bacterial enteritis in humans. Despite being considered an obligate microaerobic microorganism, C. jejuni is regularly exposed to oxidative stress. However, its adaptive strategies to survive the atmospheric oxygen level during transmission to humans remain unclear. Recently, the clinical C. jejuni strain Bf was singled out for its unexpected ability to grow under ambient atmosphere. Here, we aimed to understand better the biological mechanisms underlying its atypical aerotolerance trait using two-dimensional protein electrophoresis, gene expression, and enzymatic activities. Forty-seven proteins were identified with a significantly different abundance between cultivation under microaerobic and aerobic conditions. The over-expressed proteins in aerobiosis belonged mainly to the oxidative stress response, enzymes of the tricarboxylic acid cycle, iron uptake, and regulation, and amino acid uptake when compared to microaerobic conditions. The higher abundance of proteins related to oxidative stress was correlated to dramatically higher transcript levels of the corresponding encoding genes in aerobic conditions compared to microaerobic conditions. In addition, a higher catalase-equivalent activity in strain Bf was observed. Despite the restricted catabolic capacities of C. jejuni, this study reveals that strain Bf is equipped to withstand oxidative stress. This ability could contribute to emergence and persistence of particular strains of C. jejuni throughout food processing or macrophage attack during human infection.

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Draft Genome Sequence of Campylobacter jejuni Bf, an Atypical Strain Able To Grow under Aerobiosis

Cited by 7 publications

References 9 publications

Adhesion, Biofilm Formation, and Genomic Features of Campylobacter jejuni Bf, an Atypical Strain Able to Grow under Aerobic Conditions

Adhesion, Biofilm Formation, and Genomic Features of Campylobacter jejuni Bf, an Atypical Strain Able to Grow under Aerobic Conditions

Recent Advances in Screening of Anti-Campylobacter Activity in Probiotics for Use in Poultry

Comparison of Proteomics Profiles of Campylobacter jejuni Strain Bf under Microaerobic and Aerobic Conditions

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