Draft Genome Sequence of
            <i>Bacillus subtilis</i>
            TLO3, Isolated from Olive Tree Rhizosphere Soil

Choubane, Slimane; Gabed, Noujoud; Khelil, Omar; Cheba, Ben Amar

doi:10.1128/mra.00852-18

Cited by 4 publications

(2 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on the literature one would expect this to be an engineered strain with limited viability and abnormal cell morphology but it is a soil isolate with normal cell morphology. 49 It is an interesting question whether the one clade VII strain is deficient in WTAs but compensating with LTAs.…”

Section: Resultsmentioning

confidence: 99%

Horizontal transfer and evolution of wall teichoic acid gene cassettes in Bacillus subtilis

et al. 2021

View full text Add to dashboard Cite

Background: Wall teichoic acid (WTA) genes are essential for production of cell walls in gram-positive bacteria and necessary for survival and variability in the cassette has led to recent antibiotic resistance acquisition in pathogenic bacteria. Methods: Using a pan-genome approach, we examined the evolutionary history of WTA genes in Bacillus subtilis ssp. subtilis. Results: Our analysis reveals an interesting pattern of evolution from the type-strain WTA gene cassette possibly resulting from horizontal acquisition from organisms with similar gene sequences. The WTA cassettes have a high level of variation which may be due to one or more independent horizontal transfer events during the evolution of Bacillus subtilis ssp. subtilis. This swapping of entire WTA cassettes and smaller regions within the WTA cassettes is an unusual feature in the evolution of the Bacillus subtilis genome and highlights the importance of horizontal transfer of gene cassettes through homologous recombination within B. subtilis or other bacterial species. Conclusions: Reduced sequence conservation of these WTA cassettes may indicate a modified function like the previously documented WTA ribitol/glycerol variation. An improved understanding of high-frequency recombination of gene cassettes has ramifications for synthetic biology and the use of B. subtilis in industry.

show abstract

Section: Resultsmentioning

confidence: 99%

Horizontal transfer and evolution of wall teichoic acid gene cassettes in Bacillus subtilis

et al. 2021

View full text Add to dashboard Cite

show abstract

“…In comparison, the number of CAZyme genes for B. subtilis TLO3, B. subtilis 30VD-1, B. subtilis Gd7, and B. subtilis CRN1 were 140, 141, 143, and 143, respectively (Table 2 ). In the genome of B. subtilis TL03, coding genes related to starch and cellulose hydrolysis were detected, making the strain a potential candidate for industrial degradation of lignocellulosic biomass [ 39 ]. B. subtilis 30VD-1, isolated from the palm rhizosphere, showed strong activities of cellulase, xylanase, pectinase, and chitinase [ 40 ].…”

Section: Resultsmentioning

confidence: 99%

Whole genome sequencing and the lignocellulose degradation potential of Bacillus subtilis RLI2019 isolated from the intestine of termites

Liu,

Zhang,

Gong

et al. 2023

Biotechnol Biofuels

View full text Add to dashboard Cite

Background Lignocellulosic biomass is the most abundant and renewable terrestrial raw material for conversion into bioproducts and biofuels. However, the low utilization efficiency of lignocellulose causes environmental pollution and resource waste, which limits the large-scale application of bioconversion. The degradation of lignocellulose by microorganisms is an efficient and cost-effective way to overcome the challenge of utilizing plant biomass resources. This work aimed to screen valuable cellulolytic bacteria, explore its molecular mechanism from genomic insights, and investigate the ability of the strain to biodegrade wheat straw. Results Bacillus subtilis (B. subtilis) RLI2019 was isolated from the intestine of Reticulitermes labralis. The strain showed comprehensive enzyme activities related to lignocellulose degradation, which were estimated as 4.06, 1.97, 4.12, 0.74, and 17.61 U/mL for endoglucanase, β-glucosidase, PASC enzyme, filter paper enzyme, and xylanase, respectively. Whole genome sequencing was performed to better understand the genetic mechanism of cellulose degradation. The genome size of B. subtilis RLI2019 was 4,195,306 bp with an average GC content of 43.54%, and the sequence characteristics illustrated an extremely high probability (99.41%) as a probiotic. The genome contained 4,381 protein coding genes with an average GC content of 44.20%, of which 145 genes were classified into six carbohydrate-active enzyme (CAZyme) families and 57 subfamilies. Eight cellulose metabolism enzyme-related genes and nine hemicellulose metabolism enzyme-related genes were annotated by the CAZyme database. The starch and sucrose metabolic pathway (ko00500) was the most enriched with 46 genes in carbohydrate metabolism. B. subtilis RLI2019 was co-cultured with wheat straw for 7 days of fermentation, the contents of neutral detergent fiber, acid detergent fiber, hemicellulose, and lignin were significantly reduced by 5.8%, 10.3%, 1.0%, and 4.7%, respectively. Moreover, the wheat straw substrate exhibited 664.9 μg/mL of reducing sugars, 1.22 U/mL and 6.68 U/mL of endoglucanase and xylanase activities, respectively. Furthermore, the fiber structures were effectively disrupted, and the cellulose crystallinity was significantly reduced from 40.2% to 36.9%. Conclusions The complex diversity of CAZyme composition mainly contributed to the strong cellulolytic attribute of B. subtilis RLI2019. These findings suggest that B. subtilis RLI2019 has favorable potential for biodegradation applications, thus it can be regarded as a promising candidate bacterium for lignocellulosic biomass degradation.

show abstract

UV mutagenesis for the overproduction of thermoalkali-stable α-amylase from Bacillus subtilis TLO3 by fermentation of stale bread: Potential application as detergent additive

Khelil

Choubane

Maredj

et al. 2022

Biocatalysis and Agricultural Biotechnology

View full text Add to dashboard Cite

Draft Genome Sequence of Bacillus subtilis TLO3, Isolated from Olive Tree Rhizosphere Soil

Cited by 4 publications

References 11 publications

Horizontal transfer and evolution of wall teichoic acid gene cassettes in Bacillus subtilis

Horizontal transfer and evolution of wall teichoic acid gene cassettes in Bacillus subtilis

Whole genome sequencing and the lignocellulose degradation potential of Bacillus subtilis RLI2019 isolated from the intestine of termites

UV mutagenesis for the overproduction of thermoalkali-stable α-amylase from Bacillus subtilis TLO3 by fermentation of stale bread: Potential application as detergent additive

Contact Info

Product

Resources

About