UV mutagenesis for the overproduction of thermoalkali-stable α-amylase from Bacillus subtilis TLO3 by fermentation of stale bread: Potential application as detergent additive

Khelil, Omar; Choubane, Slimane; Maredj, Kheira; Mahiddine, Fatima Zohra; Hamouta, Ayoub

doi:10.1016/j.bcab.2022.102403

Cited by 5 publications

(2 citation statements)

References 36 publications

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“…In another study, using Bacillus subtilis [61] to produce α-amylase, it was shown that stale bread had the highest amylolytic activity (107.3 U/min), whereas the potato peel yielded 55.5 U/min. Furthermore, Benabda et al [62] demonstrated that SSF from Rhizopus oryzae produced α-amylase (100 U/g) using bread waste as a substrate, which is very similar to our SSF results on leftover bread.…”

Section: Resultsmentioning

confidence: 99%

Extracellular enzymes producing yeasts study: cost-effective production of α-amylase by a newly isolated thermophilic yeast Geotrichum candidum PO27

Chaib,

Dakhmouche-Djekrif,

Bennamoun

et al. 2024

AIMSMICRO

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<abstract> Enzymes are biocatalysts mainly used for their industrial potential in various applications. The present study aims to understand the enzyme production for biotechnological interest from a local yeast strain. From 100 isolates obtained from various biotopes, 78 strains were selected for their enzymatic heritage. Screening of α-amylase, lipase/esterase, and cellulase activities by rapid plate detection methods was carried out and the PO27 yeast was selected for its high capacity to produce α-amylase. In addition, this yeast strain exhibited good lipolytic and esterolytic activities, as well as low cellulase activity. A sequence analysis of the D1/D2 region of the 26S ribosomal RNA (26S rRNA) and a study of morphological characteristics identified the PO27 strain as <italic>Geotrichum candidum</italic>. The production of α-amylase has been studied in solid medium fermentation using various natural substrates without any supplementation such as olive pomace, potato peels, leftover bread, and mastic cake. <italic>G. candidum</italic> PO27 showed an improved production of α-amylase with olive pomace, thus reaching approximately 180.71 U/g. To evaluate the ability of this isolate to produce α-amylase in submerged fermentation, multiple concentrations of olive pomace substrate were tested. The best activity of submerged fermentation was statistically compared to the solid-state fermentation result in order to select the appropriate fermentation type. A high significant difference was found to rank the 6% olive pomace medium as the best substrate concentration with 34.395 U/mL of α-amylase activity. This work showed that the new isolate <italic>Geotrichum candidum</italic> PO27 has a better potential to produce α-amylase at a low cost in solid-state fermentation compared to submerged fermentation. Optimization conditions for PO27 α-amylase production through solid-state fermentation were achieved ‎ using a one factor at a time (OFAT) approach. The findings revealed that a high temperature (60 °C), an acidic pH, malt extract, and soluble starch were the highly significant medium components for enhancing α-‎amylase production. The use of olive pomace waste by <italic>Geotrichum candidum</italic> PO27 is expected to be effective in producing an industrially useful α-amylase. </abstract>

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Section: Resultsmentioning

confidence: 99%

Extracellular enzymes producing yeasts study: cost-effective production of α-amylase by a newly isolated thermophilic yeast Geotrichum candidum PO27

Chaib,

Dakhmouche-Djekrif,

Bennamoun

et al. 2024

AIMSMICRO

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“…Our results are supported by previous studies ( 18 ), who reported tannase, protease, amylase, xylanase, lipase, and endoglucanase activities for the bacteria extracted from the koala’s intestine. The members of Ornithinibacillus and B. subtilis isolated from the ruminants are well known to show tannase, protease, amylase and lipase activities ( 49 – 51 ). Recently, a proteolytic strain, O. caprae has been tested to degrade poultry feathers ( 52 ).…”

Section: Discussionmentioning

confidence: 99%

Screening and evaluation of the ruminal cellulolytic bacteria and their potential application as probiotics

Ghiasi,

Gheibipour,

Motamedi

et al. 2024

IJM

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Background and Objectives: Rumen microbiologists are looking for new probiotics to improve the digestibility of livestock diets. This study intended to screen and evaluate the ruminal cellulolytic bacteria (CBs) and their potential application as probiotics. Materials and Methods: Microbial culture and molecular techniques performed to isolate CBs from the rumen of camels, deer and rams. Their antibacterial and antibiogram tests were done using disc diffusion method. Their potential to degrade cellulose, starch, tannin and protein were investigated using clear zone halo, and spectrophotometric techniques. Bilious, saline, and acidic broth media were used to study the resistance of isolates in intestinal conditions. Results: The phylogenetic analysis revealed that the strains belonged to Firmicutes and Proteobacteria phyla, Citrobacter murliniae, Ornithinibacillus bavariensis, C. braakii, and Bacillus subtilis. The highest cellulase (CAS) activity was recorded by C. murliniae Dez wildlife13A (2.98 UmL-1), whereas C. braakii Loot desert 111A (1.14 Uml-1) was produced the lowest enzyme. The isolates were highly resistant to synthetic conditions of intestine (pH 2.5-3.5, bile 0.3-2%), as well as tolerated higher concentrations of NaCl (up to 10%). They effectively inhibited standard pathogen strains, and showed sensitivity to the used antibiotics. Conclusion: This study reports the cellulolytic O. bavariensis Tabbas desert 32A for the first time from the rumen, which will have potential biotechnological applications.

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Bacillus subtilis Cell Factory

Mishra¹,

Bhatt²,

Paul³

2023

Biomanufacturing for Sustainable Production of Biomolecules

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UV mutagenesis for the overproduction of thermoalkali-stable α-amylase from Bacillus subtilis TLO3 by fermentation of stale bread: Potential application as detergent additive

Cited by 5 publications

References 36 publications

Extracellular enzymes producing yeasts study: cost-effective production of α-amylase by a newly isolated thermophilic yeast <i>Geotrichum candidum</i> PO27

Extracellular enzymes producing yeasts study: cost-effective production of α-amylase by a newly isolated thermophilic yeast <i>Geotrichum candidum</i> PO27

Screening and evaluation of the ruminal cellulolytic bacteria and their potential application as probiotics

Bacillus subtilis Cell Factory

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