Draft Genome Sequence of Fusobacterium nucleatum subsp.
            <i>animalis</i>
            ChDC F324, Isolated from a Human Subgingival Plaque in the Republic of Korea

Park, Soon-Nang; Cho, Eugene; Kim, Hwa-Sook; Kim, Daesoo; Jung, Jaeeun; Baek, Jeong-Hun; Lim, Yun Kyong; Jo, Eojin; Chang, Young-Hyo; Shin, Jeong Hwan; Choi, Sang-Haeng; Kang, Jihee; Choi, YongUn; Kong, Si-Won; Han, S.; Park, Hong-Seog; Kim, Hongik; Kook, Joong-Ki

doi:10.1128/genomea.01042-13

Cited by 7 publications

(8 citation statements)

References 3 publications

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“…In order to evaluate the antibacterial properties of PHMB-coated implant surfaces, the CFU of F. nucleatum was assessed. This bacteria is a known periodontal pathogen and has also been strongly associated with peri-implantitis [34,35]. Previous studies addressing the antimicrobial properties of implants have similarly investigated the presence of this bacteria [36,37].…”

Section: Discussionmentioning

confidence: 99%

Self-Assembled PHMB Titanium Coating Enables Anti-Fusobacterium nucleatum Strategy

et al. 2021

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Fusobacterium nucleatum (F. nucleatum) is a gram-negative obligate anaerobe bacterium that threatens human periodontal health. It can cause many oral diseases, including periodontitis, gingivitis and peri-implantitis, and even some diseases such as colorectal cancer are related to it. This paper aims to develop a novel and simple surface modification method for anti-Fusobacterium nucleatum on titanium, i.e., the material for implants. In this study, different concentrations (0.0–1.0%) of PHMB were dip-coated on the titanium surface. The surface properties were examined with the aid of Scanning electron microscopy, Energy-dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy, and the antibacterial property against F. nucleatum was investigated using colony-forming unit. It was found that the PHMB successfully formed a self-assembled coating on the titanium surface and the PHMB-coated titanium had a strong capability of inhibiting F. nucleatum. Even though differences were found among the several concentrations, PHMB exhibited promising results as a simple coating strategy for dental implants.

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Section: Discussionmentioning

confidence: 99%

Self-Assembled PHMB Titanium Coating Enables Anti-Fusobacterium nucleatum Strategy

et al. 2021

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“…The FnaPatB1 sequence was identified from the genome of the F. nucleatum subspecies animalis ChDC F324 . A DNA sequence coding for a hexa-histidine tag was added to the DNA sequence of FnaPatB1 to provide a hexahistidine tag at the C-terminus of the resultant recombinant protein.…”

Section: Methodsmentioning

confidence: 99%

“…nucleatum subspecies nucleatum. However, four subspecies have been described in the oral cavity, namely nucleatum, vicentii, polymorphum, and animalis, which could also express C–S lyases with properties to be investigated. − …”

Section: Introductionmentioning

confidence: 99%

Metabolism of Cysteine Conjugates and Production of Flavor Sulfur Compounds by a Carbon–Sulfur Lyase from the Oral Anaerobe Fusobacterium nucleatum

Neiers

Gourrat

Canon

et al. 2022

J. Agric. Food Chem.

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Flavor perception is a key factor in the acceptance or rejection of food. Aroma precursors such as cysteine conjugates are present in various plant-based foods and are metabolized into odorant thiols in the oral cavity. To date, the involved enzymes are unknown, despite previous studies pointing out the likely involvement of carbon–sulfur lyases (C–S lyases) from the oral microbiota. In this study, we show that saliva metabolizes allyl-cysteine into odorant thiol metabolites, with evidence suggesting that microbial pyridoxal phosphate-dependent C–S lyases are involved in the enzymatic process. A phylogenetic analysis of PatB C–S lyase sequences in four oral subspecies of Fusobacterium nucleatum was carried out and led to the identification of several putative targets. FnaPatB1 from F. nucleatum subspecies animalis, a putative C–S lyase, was characterized and showed high activity with a range of cysteine conjugates. Enzymatic and X-ray crystallographic data showed that FnaPatB1 metabolizes cysteine derivatives within a unique active site environment that enables the formation of flavor sulfur compounds. Using an enzymatic screen with a library of pure compounds, we identified several inhibitors able to reduce the C–S lyase activity of FnaPatB1 in vitro, which paves the way for controlling the release of odorant sulfur compounds from their cysteine precursors in the oral cavity.

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“…vincentii ( 7 ), based on DNA-DNA hybridization comparisons and phenotypic characteristics ( 8 ). The representative genome sequences are available for each subspecies ( 9 – 16 ).…”

Section: Genome Announcementmentioning

confidence: 99%

Genome Sequence of Fusobacterium nucleatum Strain W1481, a Possible New Subspecies Isolated from a Periodontal Pocket

et al. 2014

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Fusobacterium nucleatum is a bacterial species commonly detected in dental plaque within the human oral cavity, with some strains associated with periodontal disease, one of the most common clinical bacterial infections in the human body. The exact mechanisms of its pathogenesis are still not completely understood. In this study, we present the genome sequence and annotation of F. nucleatum strain W1481, isolated from a periodontal pocket of a dental patient at the University of Bristol, United Kingdom, the 16S rRNA gene sequencing of which showed it to be markedly different from the five previously named subspecies.

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Draft Genome Sequence of Fusobacterium nucleatum subsp. animalis ChDC F324, Isolated from a Human Subgingival Plaque in the Republic of Korea

Cited by 7 publications

References 3 publications

Self-Assembled PHMB Titanium Coating Enables Anti-Fusobacterium nucleatum Strategy

Self-Assembled PHMB Titanium Coating Enables Anti-Fusobacterium nucleatum Strategy

Metabolism of Cysteine Conjugates and Production of Flavor Sulfur Compounds by a Carbon–Sulfur Lyase from the Oral Anaerobe Fusobacterium nucleatum

Genome Sequence of Fusobacterium nucleatum Strain W1481, a Possible New Subspecies Isolated from a Periodontal Pocket

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