Draft Genome Sequence of Bacillus pumilus BA06, a Producer of Alkaline Serine Protease with Leather-Dehairing Function

Zhao, Chuan-Wu; Wang, Haiyan; Zhang, Yizheng; Hong, Feng

doi:10.1128/jb.01694-12

Cited by 18 publications

(15 citation statements)

References 16 publications

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“…Dehairing enzymes from Bacillus sp. have also been reported in the literature [14][15][16]. Furthermore, a recent patent proposed a concrete mixture of dehairing enzymes from B. subtilis and B. cereus with sodium carbonate, caustic soda, and thioglycolic acid [17].…”

Section: Introductionmentioning

confidence: 96%

A novel keratinase from Bacillus tequilensis strain Q7 with promising potential for the leather bating process

Jaouadi

Rekik

Elhoul

et al. 2015

International Journal of Biological Macromolecules

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Section: Introductionmentioning

confidence: 96%

A novel keratinase from Bacillus tequilensis strain Q7 with promising potential for the leather bating process

Jaouadi

Rekik

Elhoul

et al. 2015

International Journal of Biological Macromolecules

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“…To date, 22 genomes of strains and isolates of B. pumilus have been sequenced, including isolate BA06 [55], the progenitor of strain SCU11. Nevertheless, studies focusing on the extracellular proteome and related secretory systems of B. pumilus remain inadequate.…”

Section: Introductionmentioning

confidence: 99%

Extracellular Proteome Profiling of Bacillus pumilus SCU11 Producing Alkaline Protease for Dehairing

Wang

Song

et al. 2016

Journal of Microbiology and Biotechnology

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is one of the most characterized microorganisms that are used for high-level production of select industrial enzymes. A novel SCU11 strain possessing high alkaline protease activity was obtained in our previous work. The culture supernatant of this strain showed efficient dehairing capability with minimal collagen damage, indicating promising potential applications in the leather industry. In this study, the strain's extracellular proteome was identified by LC-MS/MS-based shotgun proteomic analysis, and their related secretory pathways were characterized by BLAST searches. A total of 513 proteins, including 100 actual secreted and 413 intracellular proteins, were detected in the extracellular proteome. The functions of these secreted proteins were elucidated and four complete secretory systems (Sec, Tat, Com, and ABC transporter) were proposed for. These data provide a comprehensive extracellular proteome profile, which is a valuable theoretical and applicative basis for future genetic modifications and development of industrial enzymes.

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“…According to the genome sequence (Zhao et al, 2012), the strain BA06 possesses several genes encoding extracellular protease homologous to these genes (aprE, epr, vpr, wprA, aprX) in the B. subtilis strain 168, all of which were predicated to encode proteases with a secretary signal peptide (data not shown), and their products were detected in the extracellular proteome of BA06 (Yu, 2012). Therefore, real-time PCR was applied to analyze their transcriptions under salt stress.…”

Section: Transcription Of Protease-encoding Genes Under Salt Stressmentioning

confidence: 99%

“…Since genome sequencing revealed that B. pumilus BA06 possesses several putative genes encoding extracellular proteases (Zhao et al, 2012), zymogram analysis has been per- Figure 2. Repression of extracellular protease production by Bacillus pumilus BA06 following salt stress both in fermentation broth (A) and on milk-containing agar plates (B).…”

Section: Zymogram Analysis Of Extracellular Proteases Under Salt Stressmentioning

confidence: 99%

“…pumilus is recognized as being similar to the model species B. subtilis based on phylogenetic analysis (Berkeley et al, 2008). Using comparable genome analysis, several genes encoding putative extracellular proteases were revealed in the genome of B. pumilus (Zhao et al, 2012). In order to provide insight into the transcriptional regulation of extracellular protease genes, the production and transcription of these protease-encoding genes under salt stress were investigated in B. pumilus BA06.…”

Section: Introductionmentioning

confidence: 99%

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Salt stress represses production of extracellular proteases in Bacillus pumilus

Liu¹,

Huang²,

Hong³

2015

Genet. Mol. Res.

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ABSTRACT.Bacillus pumilus is able to secrete subtilisin-like proteases, one of which has been purified and characterized biochemically, demonstrating great potential for use in industrial applications. In the current study, the biosynthesis and transcription of extracellular proteases in B. pumilus (BA06) under salt stress were investigated using various methods, including a proteolytic assay, zymogram analysis, and real-time PCR. Our results showed that total extracellular proteolytic activity, both in fermentation broth and on milk-containing agar plates, was considerably repressed by salt in a dosage-dependent manner. As Bacillus species usually secret multiple extracellular proteases, a variety of individual extracellular protease encoding genes were selected for real-time PCR analysis. It was shown that proteases encoded by the aprE and aprX genes were the major proteases in the fermentation broth in terms of their transcripts in B. pumilus. Further, transcription of aprE, aprX, and epr genes was indeed repressed by salt stress. In contrast, transcription of other genes (e.g., vpr and wprA) was not repressed or significantly affected by the salt. Conclusively, salt stress represses total extracellular proteolytic activity in B. pumilus, which can largely be ascribed to suppression of the major protease-encoding genes (aprE, R.F. Liu et al. 4940©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 4939-4948 (2015) aprX) at the transcriptional level. In contrast, transcription of other protease-encoding genes (e.g., vpr, wprA) was not repressed by salt stress.

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Draft Genome Sequence of Bacillus pumilus BA06, a Producer of Alkaline Serine Protease with Leather-Dehairing Function

Cited by 18 publications

References 16 publications

A novel keratinase from Bacillus tequilensis strain Q7 with promising potential for the leather bating process

A novel keratinase from Bacillus tequilensis strain Q7 with promising potential for the leather bating process

Extracellular Proteome Profiling of Bacillus pumilus SCU11 Producing Alkaline Protease for Dehairing

Salt stress represses production of extracellular proteases in Bacillus pumilus

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