Extracellular Proteome Profiling of Bacillus pumilus SCU11 Producing Alkaline Protease for Dehairing

Wang, Chao; Yu, Shuang; Song, Ting; He, Ting; Shao, Huanhuan; Wang, Haiyan

doi:10.4014/jmb.1602.02042

Cited by 9 publications

(4 citation statements)

References 52 publications

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“…Excellent hair removal efficiency with proteinases from bacteria such as B. mojavensis SA has been reported ( Hammami et al, 2018 ). Similar properties have likewise been established in Bacillus aerius NSMk2 ( Bhari et al, 2019 ), Bacillus thuringiensis ( Agasthya et al, 2013 ), B. pumilus SCU11 ( Wang et al, 2016 ), Bacillus sp., SB12 ( Briki et al, 2016 ), and B. subtilis KT004404 ( Rehman et al, 2017 ).…”

Section: Bacillary Enzymes In Agriculturesupporting

confidence: 70%

The Practical Potential of Bacilli and Their Enzymes for Industrial Production

Danilova

Шарипова

2020

Front. Microbiol.

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Bacillus spp. are an affordable source of enzymes due to their wide distribution, safety in work, ease of cultivation, and susceptibility to genetic transformations. Researchers are particularly interested in proteolytic enzymes, which constitute one of the most diverse groups of microbial proteins in terms of properties. Despite the long history of their research, this group of enzymes continue to show great potential for practical application in the biomedical industry, as well as in the agricultural industry. Thus, the unique properties of bacillary proteinases, such as stability in a wide range of temperatures and pH, high specificity, biodegradability of a wide range of substrates, and the high potential of sequenced Bacillus genomes are a powerful foundation for the development of new biotechnologies. The current review aims to discuss recent studies on various enzymes in particular, proteinases produced by bacteria of the genus Bacillus , along with their prospective practical applications. This article also presents an interpretive summary of the recent developments on the usage of probiotic Bacillus strains as potential feed additives.

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Section: Bacillary Enzymes In Agriculturesupporting

confidence: 70%

The Practical Potential of Bacilli and Their Enzymes for Industrial Production

Danilova

Шарипова

2020

Front. Microbiol.

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“…Previously, AprE, Epr, WprA, and Vpr were identified to be extracellular protease in B. pumilus SCU11 [57]. Our transcriptome data indicated that the expression patterns of various extracellular proteases were different in B. pumilus (Table 3).…”

Section: Discussionmentioning

confidence: 63%

“…For example, AprE, Vpr, and aprX may be secreted via this system [63]. However, 65 proteins were detected from the late exponential growth cultures in B. pumilus ; this number is much lower than the predicted protein species (513) [57]. Therefore, the Sec-dependent protein secretion system may be the major pathway, especially during the stationary growth phase in B. pumilus .…”

Section: Discussionmentioning

confidence: 99%

Transcriptome profiling analysis reveals metabolic changes across various growth phases in Bacillus pumilus BA06

et al. 2017

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Background Bacillus pumilus can secret abundant extracellular enzymes, and may be used as a potential host for the industrial production of enzymes. It is necessary to understand the metabolic processes during cellular growth. Here, an RNA-seq based transcriptome analysis was applied to examine B. pumilus BA06 across various growth stages to reveal metabolic changes under two conditions.ResultsBased on the gene expression levels, changes to metabolism pathways that were specific to various growth phases were enriched by KEGG analysis. Upon entry into the transition from the exponential growth phase, striking changes were revealed that included down-regulation of the tricarboxylic acid cycle, oxidative phosphorylation, flagellar assembly, and chemotaxis signaling. In contrast, the expression of stress-responding genes was induced when entering the transition phase, suggesting that the cell may suffer from stress during this growth stage. As expected, up-regulation of sporulation-related genes was continuous during the stationary growth phase, which was consistent with the observed sporulation. However, the expression pattern of the various extracellular proteases was different, suggesting that the regulatory mechanism may be distinct for various proteases. In addition, two protein secretion pathways were enriched with genes responsive to the observed protein secretion in B. pumilus. However, the expression of some genes that encode sporulation-related proteins and extracellular proteases was delayed by the addition of gelatin to the minimal medium.ConclusionsThe transcriptome data depict global alterations in the genome-wide transcriptome across the various growth phases, which will enable an understanding of the physiology and phenotype of B. pumilus through gene expression.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-017-1066-7) contains supplementary material, which is available to authorized users.

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“…In addition, proteases control the activation, synthesis, and turnover of proteins to regulate physiological processes (Rawlings et al 2004), which explains the high number of isolates found (Bacillus, Brevibacillus, Microbacterium, Oxalicibacterium-59%) with proteolytic ability, the majority being Bacillus sp. Many authors cited this genus as a producer of alkaline proteases (active at alkaline pH ranging from 9 to 11), which are vital for industrial biocatalysis (Singhal et al 2012;Wang et al 2016).…”

Section: Discussionmentioning

confidence: 99%

Determination of biotechnological potential of poultry litter isolated bacteria

Battisti,

Junkerfuerbom,

Machado

et al. 2024

Biosci. J.

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Poultry litter is composed of residues from industrial wood processing, serving as a bed for the animals. It has also been widely used as an agricultural biofertilizer, increasing its economic importance. Few studies report the microbiological composition of this material or its biotechnological potential, which was the objective of this study. The microbiological composition was obtained by 16sRNA genetic characterization, as well as, phosphate solubilization capacity, indole-3-acetic acid (IAA) production, fungal antagonism, and enzymatic capacity. As a result, 67 isolates were obtained, of which 30 were taxonomically positioned and 27 were biochemically characterized. The microbiota genera consisted of Staphylococcus (17%), Brevibacillus (27%), Bacillus (37%), Mammaliicoccus (7%), Isoptericola, Microbacterium, and Oxalicibacterium (3.5% each). None of the isolates presented phosphate solubilization capacity. Regarding IAA production, UFPRCA19 showed the highest IAA content (16.60 µg.mg-1 protein). Eleven isolates were positive for antagonism with Aspergillus spp. and 25 for Trichoderma spp. Activity of proteases, caseinases, lipases, amylases, and cellulases were observed with the relative frequencies of 59%, 29%, 11%, 32%, 18%, and 21%. IAA and enzyme-producing bacteria as well as fungal antagonism were found among the isolates. Among all the isolated genera, Bacillus for synthesizing IAA and commercial enzymes becomes an interesting source for biotechnological purposes.

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Extracellular Proteome Profiling of Bacillus pumilus SCU11 Producing Alkaline Protease for Dehairing

Cited by 9 publications

References 52 publications

The Practical Potential of Bacilli and Their Enzymes for Industrial Production

The Practical Potential of Bacilli and Their Enzymes for Industrial Production

Transcriptome profiling analysis reveals metabolic changes across various growth phases in Bacillus pumilus BA06

Determination of biotechnological potential of poultry litter isolated bacteria

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