Draft Genome Sequence of an Invasive Multidrug-Resistant Strain, Pseudomonas aeruginosa BK1, Isolated from a Keratitis Patient

Jeganathan, Lakshmi Priya; Prakash, Logambiga; Sivakumar, Neelamegam; Antony, Aju; Al-Qarawi, S. M.; Prajna, Lalitha; Devarajan, Bharanidharan; Mohankumar, Vidyarani

doi:10.1128/genomea.00153-14

Cited by 2 publications

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“…Within the newly identified second cluster, pairwise sequence identities between the proteins from strains BK1 (Jeganathan et al. 2014 ), BWHPSA007 (with two homologues) and BWH033 ( Pseudomonas aeruginosa initiative, Broad Institute), and VRFPA06 (Murugan et al. 2014 ) exceed 95%.…”

Section: Resultsmentioning

confidence: 99%

O serotype‐independent susceptibility of Pseudomonas aeruginosa to lectin‐like pyocins

et al. 2014

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Lectin-like bacteriocins of the LlpA family, originally identified in plant-associated bacteria, are narrow-spectrum antibacterial proteins composed of two tandemly organized monocot mannose-binding lectin (MMBL) domains. The LlpA-like bacteriocin of Pseudomonas aeruginosa C1433, pyocin L1, lacks any similarity to known P. aeruginosa bacteriocins. The initial interaction of pyocin L1 with target cells is mediated by binding to d-rhamnose, present in the common polysaccharide antigen of lipopolysaccharides (LPS), but the actual cytotoxic mechanism is unknown. In this study, we characterized the activity range of pyocin L1 and two additional L pyocins revealed by genome mining, representing two highly diverged LlpA groups in P. aeruginosa. The recombinant proteins exhibit species-specific antagonistic activities down to nanomolar concentrations against clinical and environmental P. aeruginosa strains, including several multidrug-resistant isolates. The overlap in target strain spectrum between two close homologues of the pyocin L1 group is only minimal, contrasting with the considerable spectral redundancy of LlpA proteins reported for other Pseudomonas species. No correlation was found between L pyocin susceptibility and phylogenetic relatedness of P. aeruginosa isolates. Sensitive strains were retrieved in 13 out of 15 O serotypes tested, excluding the possibility that the highly variable and immunogenic O serotype antigen of the LPS coating would represent a dominant susceptibility-discriminating factor.

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Section: Resultsmentioning

confidence: 99%