Draft Genome Sequence of Achromobacter spanius Strain 6, a Soil Bacterium Isolated from a Hydrocarbon-Degrading Microcosm

Gunasekera, Thusitha S.; Radwan, Osman; Bowen, Loryn L.; Brown, Lisa M.; Ruiz, Oscar N.

doi:10.1128/mra.01124-18

Cited by 4 publications

(2 citation statements)

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“…Shigella and Achromobacter are common soil bacterial groups (Santamaría and Toranzos 2003;Adam et al 2014;Farhadkhani et al 2018;Egea et al 2017;Furlan and Stehling 2017;Gunasekera et al 2018;Thulasi et al 2018; Garrido-Sanz et al 2018;Fang et al 2018), and they could colonize in plant tissues through plant roots (Liu et al 2011). Pantoea, Bacillus, Burkholderia, Sphingomonas, Limnobacter, Methylobacterium and so on often separated or identified by high frequency from various plant seeds were also detected from this research (Mantica et al 2010;Zhang et al 2018), some species in these genera can also improve the plant growth and production (Liu et al 2012a(Liu et al , 2018.…”

Section: Discussionsupporting

confidence: 54%

Investigating the endophytic bacterial diversity and community structures in seeds of genetically related maize (Zea mays L.) genotypes

Liu

Yan

Zhang³

et al. 2020

3 Biotech

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This research aimed to investigate the composition and diversity of endophytic bacterial community in seeds of four hybrid maize and their parental lines, which was used to reveal the potential relationship and association of endophytic bacteria between maize genotypes and their genetic relevance. High-throughput sequencing (HTS) technology showed that a total of 1419 OTUs (46.6%) were parental lines unique and 1052 OTUs (34.5%) were hybrid varieties unique, with only 575 core OTUs revealed in all the samples. Most OTUs belonged to Proteobacteria. Enterobacter (23.2%), Shigella (21.2%), Pseudomonas (15.8%) and Achromobacter (10.1%) were the major genera; the bacterial community composition and diversity of endophytic bacteria were inconsistent among different seed genotypes. Based on principal component analysis (PCA), the results referred that the endophytic composition of hybrid sample showed obvious correlation with their female parental lines, and in 'Jingke968' and 'MC738' with the same female line the endophytic community was more similar than other hybrid samples. This was the first ever use of HTS technology for investigating the endophytic bacterial diversity and community structures in seeds of genetically related maize genotypes, it was shown that, there were core microbes shared among all genotypes of seed samples, and the female parental line was more significant to impact on the composition of their hybrid seeds than male parental line. This study would provide scientific clues for the future research on the vertical transmission of endophytes among maize generations.

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Section: Discussionsupporting

confidence: 54%

Investigating the endophytic bacterial diversity and community structures in seeds of genetically related maize (Zea mays L.) genotypes

Liu

Yan

Zhang³

et al. 2020

3 Biotech

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“…Dave et al (2014) observed an enhanced biodegradation of total polycyclic aromatic hydrocarbons (TPAHs) when using A. xylosoxidans , isolated from crude oil polluted marine sites. Gunasekera et al (2018) isolated a Achromobacter spanius strain which was present in the hydrocarbon-degrading bacterial community in a desert soil sample obtained under a fuel bladder.…”

Section: Resultsmentioning

confidence: 99%

Bioaugmentation of PAH-Contaminated Soils With Novel Specific Degrader Strains Isolated From a Contaminated Industrial Site. Effect of Hydroxypropyl-β-Cyclodextrin as PAH Bioavailability Enhancer

et al. 2019

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A PAHs-contaminated industrial soil was analyzed using PCR amplification of the gene 16S ribosomal RNA for the detection and identification of different isolated bacterial strains potentially capable of degrading PAHs. Novel degrader strains were isolated and identified as Achromobacter xylosoxidans 2BC8 and Stenotrophomonas maltophilia JR62, which were able to degrade PYR in solution, achieving a mineralization rate of about 1% day–1. A. xylosoxidans was also able to mineralize PYR in slurry systems using three selected soils, and the total extent of mineralization (once a plateau was reached) increased 4.5, 21, and 57.5% for soils LT, TM and CR, respectively, regarding the mineralization observed in the absence of the bacterial degrader. Soil TM contaminated with PYR was aged for 80 days and total extent of mineralization was reduced (from 46 to 35% after 180 days), and the acclimation period increased (from 49 to 79 days). Hydroxypropyl-ß-cyclodextrin (HPBCD) was used as a bioavailability enhancer of PYR in this aged soil, provoking a significant decrease in the acclimation period (from 79 to 54 days) due to an increase in PYR bioavailable fraction just from the beginning of the assay. However, a similar global extension of mineralization was obtained. A. xylosoxidans was then added together with HPBCD to this aged TM soil contaminated with PYR, and the total extent of mineralization decreased to 25% after 180 days, possibly due to the competitive effect of endogenous microbiota and the higher concentration of PYR in the soil solution provoked by the addition of HPBCD, which could have a toxic effect on the A. xylosoxidans strain.

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Metagenomic characterization reveals complex association of soil hydrocarbon-degrading bacteria

Ruiz¹,

Brown

Radwan

et al. 2021

International Biodeterioration & Biodegradation

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Draft Genome Sequence of Achromobacter spanius Strain 6, a Soil Bacterium Isolated from a Hydrocarbon-Degrading Microcosm

Abstract: Achromobacter spanius strain 6 is a Gram-negative soil bacterium isolated from a hydrocarbon-degrading microcosm. The draft genome sequence of A. spanius strain 6 is 6.57 Mb with a G+C content of 64.7% and 5,855 protein coding genes.

Cited by 4 publications

References 10 publications

Investigating the endophytic bacterial diversity and community structures in seeds of genetically related maize (Zea mays L.) genotypes

Investigating the endophytic bacterial diversity and community structures in seeds of genetically related maize (Zea mays L.) genotypes

Bioaugmentation of PAH-Contaminated Soils With Novel Specific Degrader Strains Isolated From a Contaminated Industrial Site. Effect of Hydroxypropyl-β-Cyclodextrin as PAH Bioavailability Enhancer

Metagenomic characterization reveals complex association of soil hydrocarbon-degrading bacteria

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