Draft Genome Sequence of a Wohlfahrtiimonas chitiniclastica Strain Isolated from Frozen Chicken in Rio De Janeiro, Brazil

Matos, Jorge; Faria, Adriana Rocha; Carvalho-Assef, Ana Paula D’Alincourt; Freitas-Almeida, Ângela Corrêa de; Albano, Rodolpho Mattos; Queiroz, Mara Lucia Penna

doi:10.1128/mra.00352-19

Cited by 5 publications

(23 citation statements)

References 19 publications

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“…Wohlfahrtiimonas chitiniclastica are described to be susceptible to the majority of known antibiotics with the exception of fosfomycin ( Schröttner et al, 2017 ; Matos et al, 2019 ); however, no comprehensive and comparative antimicrobial resistance profiling of a larger strain collection has been performed so far. Based on our in vitro susceptibility testing, all W. chitiniclastica isolates appear to be susceptible to β-lactam antibiotics such as penicillins, cephalosporines, monobactams, and carbapenems.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…In April 2021, GenBank ( Benson et al, 2013 ) lists 12 genomes of W. chitiniclastica strains while three draft genome reports were published ( Cao et al, 2013 ; Zhou et al, 2016 ; Matos et al, 2019 ). The strains are described to be susceptible to the majority of known antibiotics with the exception of fosfomycin ( Schröttner et al, 2017 ; Matos et al, 2019 ). First genome annotations revealed genes coding for macrolide-specific efflux pumps ( macA and macB ) ( Matos et al, 2019 ) and a bla VEB – 1 gene cassette which confers resistance to ceftazidime, ampicillin, and tetracycline ( Zhou et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

“…The strains are described to be susceptible to the majority of known antibiotics with the exception of fosfomycin ( Schröttner et al, 2017 ; Matos et al, 2019 ). First genome annotations revealed genes coding for macrolide-specific efflux pumps ( macA and macB ) ( Matos et al, 2019 ) and a bla VEB – 1 gene cassette which confers resistance to ceftazidime, ampicillin, and tetracycline ( Zhou et al, 2016 ). However, in-depth analysis is still required to generate a comprehensive antimicrobial resistance profile as the available antimicrobial susceptibility data are mostly based on case reports and preliminary genome annotations ( Zhou et al, 2016 ; Schröttner et al, 2017 ; Matos et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

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Identification and Antibiotic Profiling of Wohlfahrtiimonas chitiniclastica, an Underestimated Human Pathogen

et al. 2021

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In the past 12 years, several case reports have clearly demonstrated that Wohlfahrtiimonas chitiniclastica is capable of causing sepsis and bacteremia in humans. However, since most clinicians are not familiar with this species, little is known about its pathogenicity and treatment options while it is as rare but underestimated human pathogen. Therefore, a larger strain collection is required so that methods can be identified that are most suitable to obtain rapid and reliable identification. Moreover, the antimicrobial resistance profile needs to be elucidated in order to explore possible treatment options. Over a period of 6 years, we therefore have collected a total of 14 W. chitiniclastica isolates in routine diagnostics, which now served as the basis for a comprehensive characterization with respect to identification and antibiotic profiling. We compared the accuracy and convenience of several identification techniques in which MALDI-TOF MS and sequencing of the 16S rRNA gene have proven to be suitable for identification of W. chitiniclastica. In addition, whole genome sequencing (WGS)-based digital DNA-DNA hybridization (dDDH) was used as a reference method for strain identification, and surprised with the detection of a novel W. chitiniclastica subspecies. A combination of in silico and in vitro analyses revealed a first insight into the antimicrobial resistance profile and the molecular basis of antimicrobial resistance. Based on our findings, trimethoprim/sulfamethoxazole, levofloxacin, and cephalosporins (e.g., ceftazidime) may be the best antibiotics to use in order to treat infections caused by W. chitiniclastica, while resistance to fosfomycin, amikacin and tobramycin is observed.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification and Antibiotic Profiling of Wohlfahrtiimonas chitiniclastica, an Underestimated Human Pathogen

et al. 2021

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“…W. chitiniclastica can be found in Wohlfahrthia magnifica (Diptera, Sarcophagidae) a parasitic flesh fly native to continental Europe, Asia and the Mediterranean, Lucilia sericata (green bottle fly) in the Americas, Chrysomya megacephala (oriental latrine fly), Hermetia illucens (black soldier fly), and Musca domestica L. (housefly) [ 7 , 11 , 12 ]. Although most reports of W. chitiniclastica infections have been associated with the larvae of parasitic flies, this bacterium has also been isolated in retail frozen chicken meat, arsenic-affected soils in Bangladesh, and in cow [ 13 , 14 , 15 ].…”

Section: Review and Discussionmentioning

confidence: 99%

Human Infections by Wohlfahrtiimonas chitiniclastica: A Mini-Review and the First Report of a Burn Wound Infection after Accidental Myiasis in Central Europe

et al. 2021

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Wohlfahrtiimonas chitiniclastica are bacteria that cause rare infections, typically associated with the infestation of an open wound with fly larvae. Here, we present a unique case report of the first W. chitiniclastica isolation from a burn wound with accidental myiasis in a 63-year-old homeless man and a literature review focused on human infections caused by these bacteria. So far, 23 cases of infection with W. chitiniclastica have been reported; in 52% of these, larvae were found in the wound area. Most of these cases suffered from chronic non-healing wound infections but none of these were burn injuries. The overall fatality rate associated directly with W. chitiniclastica in these cases was 17%. Infections with parasitic larvae occur in moderate climates (especially in people living in poor conditions); therefore, an infection with rare bacteria associated with accidental myiasis, such as W. chitiniclastica, can be expected to become more common there. Thus, in view of the absence of recommendations regarding the treatment of patients with accidental myiasis and, therefore, the risk of infection with W. chitiniclastica or other rare pathogens, we provide a list of recommendations for the treatment of such patients. The importance of meticulous microbial surveillance using molecular biological methods to facilitate the detection of rare pathogens is emphasized.

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The zoonotic pathogen Wohlfahrtiimonas chitiniclastica – current findings from a clinical and genomic perspective

Kopf,

Bunk,

Riedel

et al. 2024

BMC Microbiol

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The zoonotic pathogen Wohlfahrtiimonas chitiniclastica can cause several diseases in humans, including sepsis and bacteremia. Although the pathogenesis is not fully understood, the bacterium is thought to enter traumatic skin lesions via fly larvae, resulting in severe myiasis and/or wound contamination. Infections are typically associated with, but not limited to, infestation of an open wound by fly larvae, poor sanitary conditions, cardiovascular disease, substance abuse, and osteomyelitis. W. chitiniclastica is generally sensitive to a broad spectrum of antibiotics with the exception of fosfomycin. However, increasing drug resistance has been observed and its development should be monitored with caution. In this review, we summarize the currently available knowledge and evaluate it from both a clinical and a genomic perspective.

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Draft Genome Sequence of a Wohlfahrtiimonas chitiniclastica Strain Isolated from Frozen Chicken in Rio De Janeiro, Brazil

Cited by 5 publications

References 19 publications

Identification and Antibiotic Profiling of Wohlfahrtiimonas chitiniclastica, an Underestimated Human Pathogen

Identification and Antibiotic Profiling of Wohlfahrtiimonas chitiniclastica, an Underestimated Human Pathogen

Human Infections by Wohlfahrtiimonas chitiniclastica: A Mini-Review and the First Report of a Burn Wound Infection after Accidental Myiasis in Central Europe

The zoonotic pathogen Wohlfahrtiimonas chitiniclastica – current findings from a clinical and genomic perspective

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