Draft genome of multiple resistance donor plant Sinapis alba: An insight into SSRs, annotations and phylogenetics

Kumari, Preetesh; Singh, Kamaldeep; Kumar, Pramod

doi:10.1371/journal.pone.0231002

Cited by 18 publications

(17 citation statements)

References 42 publications

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“…In the current study, Alternaria black spot disease infection was high on half of the tested cruciferous species, and we consider that this pathogen constitutes a direct threat to oilseed crops. Altered climatic conditions for plant growth due to global change and the growing population of the world reinforce the development of strategies for a sustainable agriculture and food system that ensure the efficient land use and high nutritional value of plants [43]. Thus, the knowledge of Alternaria black spot spread and plant vulnerability allows selection of more resistant species and cultivars, and this information is highly relevant for oilseed, intercrop, cover crop and green manure production, especially in sustainable farming systems.…”

Section: Discussionmentioning

confidence: 99%

Alternaria Black Spot (Alternaria brassicae) Infection Severity on Cruciferous Oilseed Crops

et al. 2021

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The increase in the cultivation area of cruciferous oilseed crops and the use of short crop rotation has resulted in the enhanced spread of several major pests in Northern latitudes. There is currently limited information about incidence and severity of Alternaria black spot disease (Alternaria brassicae) on the main oilseed crop, spring oilseed rape (Brassica napus), in the Northern Baltics. Thus, spring oilseed rape and five alternative cruciferous oilseed crops were selected and their resistance to black spot disease was evaluated in field conditions during two growing seasons. We hypothesized that spring oilseed rape is more susceptible to Alternaria black spot disease than other alternative cruciferous oilseed crops. Both growing seasons were warmer and drier compared to the long-term average, and were thus suitable for A. brassicae development and assessments. In both years, incidence of Alternaria black spot infection was recorded on all cruciferous species, yet the disease development differed considerably among the crops. During both growing seasons, black mustard (B. nigra) plants were the most infected. Based on our observations during warm growing seasons we conclude that alternative oilseed crops such as Sinapis alba, Eruca sativa and Raphanus sativus are more resistant to the Alternaria black spot infection than the traditional oilseed crops and thus, possess a great potential to grow with limited chemical disease control in Northern Baltic conditions.

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Section: Discussionmentioning

confidence: 99%

Alternaria Black Spot (Alternaria brassicae) Infection Severity on Cruciferous Oilseed Crops

et al. 2021

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“…Thus, they have suggested that it might be a part of the S. alba-specific gene sequence. We have used this 288 bp sequence DNA sequence in the Basic Local Alignment Search Tool Nucleotide (BLASTN) with the S. alba draft genome assembly (Kumari et al, 2020a) and found three copies of approximately similar sequences in S. alba genome. Apart from this, we have studied the histology of physiologically mature seeds of both allohexaploids (yellow and mottled seeded).…”

Section: Discussionmentioning

confidence: 99%

Development of a Yellow-Seeded Stable Allohexaploid Brassica Through Inter-Generic Somatic Hybridization With a High Degree of Fertility and Resistance to Sclerotinia sclerotiorum

et al. 2020

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The Brassica coenospeceis have treasure troves of genes that could be beneficial if introgressed into cultivated Brassicas to combat the current conditions of climate change. Introducing genetic variability through plant speciation with polyploidization is well documented, where ploidy augmentation of inter-generic allohexaploids using somatic hybridization has significantly contributed to genetic base broadening. Sinapis alba is a member of the Brassicaceae family that possesses valuable genes, including genes conferring resistance to Sclerotinia sclerotiorum, Alternaria brassicae, pod shattering, heat, and drought stress. This work aimed to synthesize stable allohexaploid (AABBSS) Brassica while incorporating the yellow-seed trait and resistance to S. sclerotiorum stem rot. The two fertile and stable allohexaploids were developed by polyethylene glycol mediated protoplast fusions between Brassica juncea (AABB) and S. alba (SS) and named as JS1 and JS2. These symmetric hybrids (2n = 60) were validated using morphological and molecular cytology techniques and were found to be stable over consecutive generations. The complete chromosome constitution of the three genomes was determined through genomic in situ hybridization of mitotic cells probed with S. alba genomic DNA labeled with fluorescein isothiocyanate. These two allohexaploids showed 24 hybridization signals demonstrating the presence of complete diploid chromosomes from S. alba and 36 chromosomes from B. juncea. The meiotic pollen mother cell showed 30 bivalent sets of all the 60 chromosomes and none of univalent or trivalent observed during meiosis. Moreover, the backcross progeny 1 plant revealed 12 hybridization signals out of a total of 48 chromosome counts. Proper pairing and separation were recorded at the meiotic metaphase and anaphase, which proved the stability of the allohexaploid and their backcross progeny. When screening, the allohexaploid (JS2) of B. juncea and S. alba displayed a high degree of resistance to S. sclerotiorum rot along with a half-yellow and half-brown (mosaic) seed coat color, while the B. juncea and S. alba allohexaplopid1 (JS1) displayed a yellow seed coat color with the same degree of resistance to Sclerotinia rot.

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“…The medicinal plant Isatis indigotica (2n = 14, Figure 2b,c ) shows resistance to diseases caused by bacteria and viruses (Kang et al ., 2020 ). White mustard ( Sinapis alba , 2n = 24) has yellow seeds, resistance to biotic and abiotic stresses, pod shatter resistance, and beneficial ingredients for humans (Kumari et al ., 2020 ). Some Brassicaceae species with insect resistance have also been identified, as reviewed in Herve ( 2018 ).…”

Section: Broadening the Genetic Diversity Of The Brassica Napus Gene Poolmentioning

confidence: 99%

Exploring the gene pool ofBrassica napusby genomics‐based approaches

Jing

Snowdon

et al. 2021

Plant Biotechnology Journal

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Summary De novo allopolyploidization in Brassica provides a very successful model for reconstructing polyploid genomes using progenitor species and relatives to broaden crop gene pools and understand genome evolution after polyploidy, interspecific hybridization and exotic introgression. B. napus (AACC), the major cultivated rapeseed species and the third largest oilseed crop in the world, is a young Brassica species with a limited genetic base resulting from its short history of domestication, cultivation, and intensive selection during breeding for target economic traits. However, the gene pool of B. napus has been significantly enriched in recent decades that has been benefit from worldwide effects by the successful introduction of abundant subgenomic variation and novel genomic variation via intraspecific, interspecific and intergeneric crosses. An important question in this respect is how to utilize such variation to breed crops adapted to the changing global climate. Here, we review the genetic diversity, genome structure, and population‐level differentiation of the B. napus gene pool in relation to known exotic introgressions from various species of the Brassicaceae, especially those elucidated by recent genome‐sequencing projects. We also summarize progress in gene cloning, trait‐marker associations, gene editing, molecular marker‐assisted selection and genome‐wide prediction, and describe the challenges and opportunities of these techniques as molecular platforms to exploit novel genomic variation and their value in the rapeseed gene pool. Future progress will accelerate the creation and manipulation of genetic diversity with genomic‐based improvement, as well as provide novel insights into the neo‐domestication of polyploid crops with novel genetic diversity from reconstructed genomes.

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Draft genome of multiple resistance donor plant Sinapis alba: An insight into SSRs, annotations and phylogenetics

Cited by 18 publications

References 42 publications

Alternaria Black Spot (Alternaria brassicae) Infection Severity on Cruciferous Oilseed Crops

Alternaria Black Spot (Alternaria brassicae) Infection Severity on Cruciferous Oilseed Crops

Development of a Yellow-Seeded Stable Allohexaploid Brassica Through Inter-Generic Somatic Hybridization With a High Degree of Fertility and Resistance to Sclerotinia sclerotiorum

Exploring the gene pool ofBrassica napusby genomics‐based approaches

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