DPPH Radical Scavenging and Postprandial Hyperglycemia Inhibition Activities and Flavonoid Composition Analysis of Hawk Tea by UPLC-DAD and UPLC-Q/TOF MSE

Xiao, Xuan; Xu, Lijia; Hu, Huagang; Yang, Yinjun; Zhang, Xinyao; Peng, Yong; Xiao, Pei‐Gen

doi:10.3390/molecules22101622

Cited by 20 publications

(20 citation statements)

References 42 publications

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“…Interesting is the fact that both kaempferol and astragalin, the kaempferol 3-O-glucoside, were detected in stems extract. Apparently, this is the first report on their occurrence in this species, however their natural occurrence is well reported and our data are in accordance with the previously reported data, which were also used to establish our assignment [35][36][37][38]. Myricetin 6-C-glucoside (Figure 8), assigned to the compound eluted at 10.89 min, was previously found in the G. tridentata extract [14,33], as well as isorhamnetin 3-O-glucoside (Figure 8), assigned to the compound eluted at 10.89 min.…”

Section: Flavonol and Flavanonol Derivativessupporting

confidence: 92%

Flavonoid Profile of the Genista tridentata L., a Species Used Traditionally to Treat Inflammatory Processes

et al. 2020

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Ethnopharmacological surveys on Portuguese flora reveal that Genista tridentata L. is a shrub used in traditional medicine for the treatment of various inflammation-related health problems, although scientific support of its benefits is still necessary. In order to establish the anti-inflammatory potential of G. tridentata and support its traditional use, ethanolic extracts of three sections of the plant (root, stem, and leaves) were subjected to in vitro evaluation of anti-inflammatory activity using lipopolysaccharide (LPS)-stimulates macrophages as an inflammation model. Simultaneously, we also aimed to establish the extracts’ flavonoids profile. The ethanolic extracts, obtained by Soxhlet extraction, profile of the three sections confirmed their richness in flavonoids, being three prenylated flavonoids isolated and characterized in the root, including a new natural compound, the 3-methoxymundulin. The extracts from the three plant sections showed strong antioxidant activity at the cellular level and significantly inhibit the LPS-triggered NO production by downregulating Nos2 gene transcription and consequently iNOS expression. Additionally, root and stem extracts also decreased the LPS-induced transcription of the pro-inflammatory genes Il1b, Il6, and Ptgs2. Thus, the results support the anti-inflammatory properties attributed to G. tridentate preparations. Relevantly, the roots of the shrub, plant part not used, is an unexplored source of compounds with pharmacological and nutraceutical value.

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Section: Flavonol and Flavanonol Derivativessupporting

confidence: 92%

Flavonoid Profile of the Genista tridentata L., a Species Used Traditionally to Treat Inflammatory Processes

et al. 2020

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“…Fourteen compounds, listed in Table S7 , were selected in negative and positive ionization modes (LC-MS) and used as target compounds to evaluate possible changes in the metabolism of P. brasiliensis during the in vitro culture establishment procedure. Compounds 1 to 7 ionized better at negative mode and were characterized as flavonoids [ 63 , 64 , 65 , 66 ] except for compound 7 that is a lignan (arabelline) according to data comparison, as described previously, or as unknown flavonoids ( 4 and 6 ). The total ion chromatograms of the different steps of the culture establishment procedure, shown in Figure 9 , were compared, and the results showed that none of the selected marker compounds observed in steps 1 and 2 at the negative mode are detected in steps 3 and 4.…”

Section: Resultsmentioning

confidence: 99%

Secure and Sustainable Sourcing of Plant Tissues for the Exhaustive Exploration of Their Chemodiversity

et al. 2020

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The main challenge of plant chemical diversity exploration is how to develop tools to study exhaustively plant tissues. Their sustainable sourcing is a limitation as bioguided strategies and dereplication need quite large amounts of plant material. We examine if alternative solutions could overcome these difficulties by obtaining a secure, sustainable, and scalable source of tissues able to biosynthesize an array of metabolites. As this approach would be as independent of the botanical origin as possible, we chose eight plant species from different families. We applied a four steps culture establishment procedure, monitoring targeted compounds through mass spectrometry-based analytical methods. We also characterized the capacities of leaf explants in culture to produce diverse secondary metabolites. In vitro cultures were successfully established for six species with leaf explants still producing a diversity of compounds after the culture establishment procedure. Furthermore, explants from leaves of axenic plantlets were also analyzed. The detection of marker compounds was confirmed after six days in culture for all tested species. Our results show that the first stage of this approach aiming at easing exploration of plant chemodiversity was completed, and leaf tissues could offer an interesting alternative providing a constant source of natural compounds.

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“…The components of the total flavonoids of tartary buckwheat were analyzed by Shimadzu UPLC-PDA system [37]. The components of BWFTs were determined by reference to quercetin and rutin.…”

Section: Methodsmentioning

confidence: 99%

The Effect of Tartary Buckwheat Flavonoids in Inhibiting the Proliferation of MGC80-3 Cells during Seed Germination

et al. 2019

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Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn) is rich in functional compounds such as rutin, quercetin, d-chiro-inositol, dietary fiber, and essential amino acids. Electric field (EF) treatment before sprout germination results in physiological and chemical changes, and some alterations might lead to positive applications in plant seeds. MTT assay showed that the effect of total flavonoids on human gastric cancer cell line MGC80-3 was significantly changed after EF treatment for different germination days (3–7 days). Among them, the total flavonoids of tartary buckwheat (BWTF) on the third day had the most obvious inhibitory effect on MGC80-3 (p < 0.01). In addition, flow cytometry evidenced that different ratios of quercetin and rutin had effects on the proliferation of MGC80-3. The same content of quercetin and rutin had the best effect, reaching 6.18 ± 0.82%. The anti-cancer mechanism was mainly promoted by promoting the expression of apoptotic proteins. The expression of Bax/Bcl-2 and caspase-8 in MGC80-3 cells was mediated by BWTFs. This study has good research value for improving the biological and economic value of tartary buckwheat.

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DPPH Radical Scavenging and Postprandial Hyperglycemia Inhibition Activities and Flavonoid Composition Analysis of Hawk Tea by UPLC-DAD and UPLC-Q/TOF MSE

Cited by 20 publications

References 42 publications

Flavonoid Profile of the Genista tridentata L., a Species Used Traditionally to Treat Inflammatory Processes

Flavonoid Profile of the Genista tridentata L., a Species Used Traditionally to Treat Inflammatory Processes

Secure and Sustainable Sourcing of Plant Tissues for the Exhaustive Exploration of Their Chemodiversity

The Effect of Tartary Buckwheat Flavonoids in Inhibiting the Proliferation of MGC80-3 Cells during Seed Germination

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