Doxycycline Counteracts Bone Morphogenic Protein 2–Induced Osteogenic Mediators

Muthukuru, Manoj; Sun, Jerry Chih‐Yuan

doi:10.1902/jop.2012.120338

Cited by 22 publications

(21 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Three-dimensional collagen gels with osteoblasts were cultured in cell culture wells of 12 mm diameter containing a 0.4 mm pore sized polyester membrane trans-wells (Corning Incorporated, Corning, NY, USA) as previously described. 10 Collagen gels were prepared by mixing 3 mg⋅mL −1 of type I rat tail collagen (Invitrogen, Carlsbad, CA, USA), α-MEM with 10% heat inactivated fetal bovine serum and osteoblast suspension (final concentration of 1.33×10 5 cells per mL). Three milliliters of gel solution was added to each transwell resulting in 4×10 5 cells per transwell.…”

Section: Methodsmentioning

confidence: 99%

“…10 (i) Histochemical staining procedure was performed by employing nitro blue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolyl phosphate (BCIP) and a substrate buffer (0.1 mol⋅L −1 Tris, 100 mmol⋅L −1 sodium chloride, 5 mmol⋅L −1 MgCl 2 , pH 9.5). The cultures were washed with hanks balanced salt solution and incubated with BCIP/NBT substrate solution for 10 min.…”

Section: Methodsmentioning

confidence: 99%

“…10 For histochemical analyses, cultures were washed three times in distilled water and stained with 2% alizarin red solution (pH 4.1–4.3) for 3 min. The excess dye was washed and the amount of orange–red calcium deposits were analyzed by capturing images with Nikon Eclipse 400 microscope at ×1 magnification.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

TLR signaling that induces weak inflammatory response and SHIP1 enhances osteogenic functions

Muthukuru

Darveau

2014

Bone Res

Self Cite

View full text Add to dashboard Cite

Toll-like receptor (TLR)-mediated inflammatory response could negatively affect bone metabolism. In this study, we determined how osteogenesis is regulated during inflammatory responses that are downstream of TLR signaling. Human primary osteoblasts were cultured in collagen gels. Pam3CSK4 (P3C) and Escherichia coli lipopolysaccharide (EcLPS) were used as TLR2 and TLR4 ligand respectively. Porphyromonas gingivalis LPS having TLR2 activity with either TLR4 agonism (Pg1690) or TLR4 antagonism (Pg1449) and mutant E. coli LPS (LPxE/LPxF/WSK) were used. IL-1β, SH2-containing inositol phosphatase-1 (SHIP1) that has regulatory roles in osteogenesis, alkaline phosphatase and mineralization were analyzed. 3α-Aminocholestane (3AC) was used to inhibit SHIP1. Our results suggest that osteoblasts stimulated by P3C, poorly induced IL-1β but strongly upregulated SHIP1 and enhanced osteogenic mediators. On the contrary, EcLPS significantly induced IL-1β and osteogenic mediators were not induced. While Pg1690 downmodulated osteogenic mediators, Pg1449 enhanced osteogenic responses, suggesting that TLR4 signaling annuls osteogenesis even with TLR2 activity. Interestingly, mutant E. coli LPS that induces weak inflammation upregulated osteogenesis, but SHIP1 was not induced. Moreover, inhibiting SHIP1 significantly upregulated TLR2-mediated inflammatory response and downmodulated osteogenesis. In conclusion, these results suggest that induction of weak inflammatory response through TLR2 (with SHIP1 activity) and mutant TLR4 ligands could enhance osteogenesis.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

TLR signaling that induces weak inflammatory response and SHIP1 enhances osteogenic functions

Muthukuru

Darveau

2014

Bone Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…We thought that increased bone formation was related to BMP-2 release. Muthukuru et al 31 reported doxycyclin was a stronger inducer of alkaline phosphatase expression but combined with BMP-2, counteracted the ınduction of osteogenic mediators. Wübbenhorst et al 32 investigated whether tetracycline had a positive effect on inducible BMP-2 expression.…”

mentioning

confidence: 99%

Effect of Local Rifamycin Application on Expression of BMP-2 and Bone Regeneration

Ün¹,

Özeç²,

Taşdemir³

et al. 2017

Cumhuriyet Dental Journal

View full text Add to dashboard Cite

Objectives: The aim of this study was to evaluate effect of local rifamycin application on BMP-2 expression and bone healing.Materials and Methods: A standardized 5.0-mm-diameter critical size bone defect was created mandible angulus region. In the control group (8 rats) defects were left empty. In the Group 1 (n=8 rats) defect was irrigated with rifamycin solution and 25 mg rifamycin solution injected defect area at 1, 3, 7 days after surgery. In the group 2 (n=8 rats) defects were grafted with a gelatin sponge mixed 25 mg rifamycin solution. Rats were sacrificed at 21 days after surgery. Histological slides were prepared from defect site for both immunohistochemical analysis (bone morphogenetic protein-2 (BMP-2) antibody) and histomorphometric analysis. Data were analyzed using Mann Whitney U and Kruskall Wallis test.

show abstract

“…Entretanto, 60 segundos de aplicação de tetraciclina na concentração usada, pode ter sido um tempo excessivamente longo de desmineralização e pode ter atrapalhado o crescimento e diferenciação celular. Uma provável explicação para esses resultados pode estar embasada em dados de outros pesquisadores que observaram que pigmentos residuais da tetraciclina incorporados à superfície óssea acarretam uma reação de tipo corpo estranho em lugar de neoformação óssea, interferindo na comunicação e expressão das BMPs e MMPs (MUTHUKURU; SUN, 2013;GOLUB et al, 1991).…”

Section: Horasunclassified

Efeito da desmineralização óssea por tetraciclina e ácido cítrico sobre a composição química superficial e sobre o comportamento de pré-osteoblastos cultivados em osso da calvária de ratos

Manfredi¹

View full text Add to dashboard Cite

Efeito da desmineralização óssea por tetraciclina e ácido cítrico sobre a composição química superficial e sobre o comportamento de pré-osteoblastos cultivados em osso da calvária de ratos BAURU 2016 GUSTAVO GONÇALVES DO PRADO MANFREDIEfeito da desmineralização óssea por tetraciclina e ácido cítrico sobre a composição química superficial e sobre o comportamento de pré-osteoblastos cultivados em osso da calvária de ratos DedicatóriaDedico àquelas pessoas que possuem vontade de aprender, e que acreditam que a eterna busca do saber é mais interessante do que o conhecido.À minha família, que faz de minha vida viável nesse mundo: vocês são minha raiz, meu tronco e meu fruto. AgradecimentosAcredito muito que os agradecimentos, além de serem ditos, devem ser representados com atitudes, gestos, sorrisos e com o sentimento de querer o melhor para a pessoa. Minha sincera gratidão a todos aqueles que de certa forma doaram um pouco de si para minha formação profissional, pessoal e para a conclusão deste trabalho.Agradeço à Profa. Dra. Maria Lúcia Rubo de Rezende (Malu), pelas conversas sobre vida, família, casos clínicos, cirurgias e seminários, das quais me fizeram refletir e ter senso crítico e, consequentemente, ajudaram na minha transição de graduando para pós-graduando com muito mais propriedade e bagagens para caminhar nessa estrada.Agradeço a todos os professores da Disciplina de Periodontia. À Profa.Dra. Adriana Campos Passanezi Sant'Ana, por seu jeito acolhedor e carinhoso de transmitir os ensinamentos da periodontia nas clínica e seminários. À Profª. Drª.Carla Andreotti Damante, por ajudar no meu processo de aprendizagem de forma leve e tranquila, e pelas orientações na cultura celular desse trabalho. À Profa. Dra.Mariana Schutzer Ragghianti Zangrando, por ser uma grande incentivadora dos alunos sempre querendo o melhor. Ao Prof. Dr. Sebastião Luíz Aguiar Greghi, pelas sabias palavras tanto na clínica quanto fora dela, que fazem termos discernimento para questões do mundo afora.Ao Prof. Dr. Paulo Noronha Lisboa Filho, pela parceria no projeto. Muito obrigado por disponibilizar o laboratório e sua equipe para esta e para as demais pesquisas realizadas por nosso grupo, sempre mostrando disposição. seconds, and C, control group of samples not demineralized. The pre-osteoblasts were cultured on the samples for 24, 48 and 72 hours (n = 6) to be examined in the scanning electron microscope. Twenty-one samples were collected from other 11 animals and were distributed among the same groups for analysis of the surface chemical composition by energy dispersive spectroscopy (EDS) (n = 3). The average percentage of the bone surfaces covered by cells was significantly higher after 24and 48 hours of culture in groups AC15 (60.38% and 100% respectively), AC30(99.32% and 100% respectively), AC60 (99.22% and 100% respectively) TCN15(96.73% and 70.24% respectively) and TCN30 (64.72% and 57.40% respectively) than in groups TCN60 (9.67% and 51.45% respectively), and C (5.99% and 31.83% respectively). At 72 hours, all the groups pres...

show abstract

Doxycycline Counteracts Bone Morphogenic Protein 2–Induced Osteogenic Mediators

Cited by 22 publications

References 47 publications

TLR signaling that induces weak inflammatory response and SHIP1 enhances osteogenic functions

TLR signaling that induces weak inflammatory response and SHIP1 enhances osteogenic functions

Effect of Local Rifamycin Application on Expression of BMP-2 and Bone Regeneration

Efeito da desmineralização óssea por tetraciclina e ácido cítrico sobre a composição química superficial e sobre o comportamento de pré-osteoblastos cultivados em osso da calvária de ratos

Contact Info

Product

Resources

About