Doxorubicin-loaded poly (lactic-co-glycolic acid) nanoparticles coated with chitosan/alginate by layer by layer technology for antitumor applications

Abstract

Natural polyelectrolyte multilayers of chitosan (CHI) and alginate (ALG) were alternately deposited on doxorubicin (DOX)-loaded poly (lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) with layer by layer self-assembly to control drug release for antitumor activity. Numerous factors which influenced the multilayer growth on nano-colloidal particles were studied: polyelectrolyte concentration, NaCl concentration and temperature. Then the growth regime of the CHI/ALG multilayers was elucidated. The coated NPs were characterized by transmission electron microscopy, atomic force microscopy, X-ray diffraction and a zeta potential analyzer. In vitro studies demonstrated an undesirable initial burst release of DOX-loaded PLGA NPs (DOX-PLGA NPs), which was relieved from 55.12% to 5.78% through the use of the layer by layer technique. The release of DOX increased more than 40% as the pH of media decreased from 7.4 to 5.0. More importantly, DOX-PLGA (CHI/ALG) 3 NPs had superior in vivo tumor inhibition rates at 83.17% and decreased toxicity, compared with DOX-PLGA NPs and DOX in solution. Thus, the presently formulated PLGA-polyelectrolyte NPs have strong potential applications for numerous controlled anticancer drug release applications.

The Potential of Atractylodin-Loaded PLGA Nanoparticles as Chemotherapeutic for Cholangiocarcinoma

“…The drug release from ALNPs-1 in PBS (pH 7.4) after 24 hours of incubation was significantly higher than that in DMEM-serum free medium (pH 8.85) and RPMI-serum free medium (pH 9.06). The higher drug release rate in PBS was due to the lower pH of PBS which facilitated hydrolysis of PLGA copolymer and resulted in higher amount of compound release from the nanoparticles compared to drug release into DMEM-serum free medium and RPMI-serum free medium (Ham et al, 2008;Khanal et al, 2016;Chai et al, 2017). However, after 48 and 72 hours, the amount of atractylodin released was comparable in all media.…”

Polysaccharide Based Nanoparticles

“…Even though Poly (lactic‐co‐glycolic acid) (PLGA) is one of the common polymer used for preparation of nano carriers and encapsulation of various anticancer drugs for their sustained release, there is a burst release of the drugs at initial. In order to prevent this Zheng's group prepared DOX loaded PLGA nanoparticles and coated their surface with multilayers of the polyelectrolytes CS and ALG. They synthesized multi‐layer coated nanoparticles with size of 200 nm and they reduced the initial burst release from 58 % to 5 %.…”

Natural material-decorated mesoporous silica nanoparticle container for multifunctional membrane-controlled targeted drug delivery

“…[36][37][38] The nanoparticles (MSN, MSN-NH 2 , or MSN-COOH) were resuspended in 10 mL ultrapure water (1 mg/mL). The first layer was deposited by adding 5 mL of CS (pH 3.5, 1 mg/mL) or HA (1 mg/mL) to the aforementioned solution.…”