Doxorubicin Hydrochloride Loaded Zymosan-Polyethylenimine Biopolymeric Nanoparticles for Dual ‘Chemoimmunotherapeutic’ Intervention in Breast Cancer

Pawar, Vivek K.; Singh, Yuvraj; Sharma, Komal; Shrivastav, Arpita; Sharma, Abhisheak; Singh, Atul Kumar; Meher, Jaya Gopal; Singh, Pankaj Kumar; Raval, Kavit; Bora, Himangshu K.; Datta, Dipak; Lal, Jawahar; Chourasia, Manish K.

doi:10.1007/s11095-017-2195-2

Cited by 14 publications

(6 citation statements)

References 59 publications

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“…Presence of PEG in template was expected to accord nanoparticles long circulation halflives, which in turn would assist in their probability of reaching tumor microenvironment 10 for targeted delivery of potent cytotoxic DOX in 4T1 cell induced allogenic breast cancer model. 11 It is assumed that these nanoparticles, after accumulation at intended destination owing to their size, would then be preferentially internalized by cancer cells due to specific interaction of their biotin component with overexpressed biotin receptors on 4T1 cells. 12 In the direction of scheme depicted in Figure 2, nanoparticles made of biotinylated−PEG-PLGA containing high load of doxorubicin hydrochloride (BPNP) were prepared via a common ion effect modified W/O/W emulsion method.…”

Section: ■ Introductionmentioning

confidence: 99%

“…We consequently decorated PLGA with biotinylated-PEG by utilizing click chemistry to generate a cancer cell homing copolymer: biotinylated–PEG-PLGA. Presence of PEG in template was expected to accord nanoparticles long circulation half-lives, which in turn would assist in their probability of reaching tumor microenvironment for targeted delivery of potent cytotoxic DOX in 4T1 cell induced allogenic breast cancer model . It is assumed that these nanoparticles, after accumulation at intended destination owing to their size, would then be preferentially internalized by cancer cells due to specific interaction of their biotin component with overexpressed biotin receptors on 4T1 cells .…”

Section: Introductionmentioning

confidence: 99%

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Click Biotinylation of PLGA Template for Biotin Receptor Oriented Delivery of Doxorubicin Hydrochloride in 4T1 Cell-Induced Breast Cancer

et al. 2017

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PLGA was functionalized with PEG and biotin using click chemistry to generate a biotin receptor targeted copolymer (biotinylated-PEG-PLGA) which in turn was used to fabricate ultrafine nanoparticles (BPNP) of doxorubicin hydrochloride (DOX) for effective delivery in 4T1 cell induced breast cancer. However, adequate entrapment of a hydrophilic bioactive like DOX in a hydrophobic polymer system made of PLGA is not usually possible. We therefore modified a conventional W/O/W emulsion method by utilizing NHCl in the external phase to constrain DOX in dissolved polymer phase by suppressing DOX's inherent aqueous solubility as per common ion effect. This resulted in over 8-fold enhancement in entrapment efficiency of DOX inside BPNP, which otherwise is highly susceptible to leakage due to its relatively high aqueous solubility. TEM and DLS established BPNP to be sized below 100 nm, storage stability studies showed that BPNP were stable for one month at 4 °C, and in vitro release suggested significant control in drug release. Extensive in vitro and in vivo studies were conducted to propound anticancer and antiproliferative activity of BPNP. Plasma and tissue distribution study supplemented by pertinent in vivo fluorescence imaging mapped the exact fate of DOX contained inside BPNP once it was administered intravenously. A comparative safety profile via acute toxicity studies in mice was also generated to out rightly establish usefulness of BPNP. Results suggest that BPNP substantially enhance anticancer activity of DOX while simultaneously mitigating its toxic potential due to altered spatial and temporal presentation of drug and consequently deserve further allometric iteration.

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Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Click Biotinylation of PLGA Template for Biotin Receptor Oriented Delivery of Doxorubicin Hydrochloride in 4T1 Cell-Induced Breast Cancer

et al. 2017

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“…Recently, many researchers have reported the successful development of co‐delivery systems loaded with traditional chemotherapeutic drugs and TAM‐specific agents for BC therapy (Table 4). Several nanoparticles, such as fucoidan, zymosan, and Fe 3 O 4 , have been reported to have the ability to deplete or reprogram TAMs 177–181 . It is advantageous to use them as carriers to deliver chemotherapy drugs to BC tissues since they not only exert the killing effect of chemotherapy on tumor cells but also improve innate immunity.…”

Section: Nddss Against Tamsmentioning

confidence: 99%

Antitumor therapy for breast cancer: Focus on tumor‐associated macrophages and nanosized drug delivery systems

Zhan

Jin

et al. 2023

Cancer Medicine

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Background In breast cancer (BC), tumor‐associated macrophages (TAMs) are an important component of the tumor microenvironment and are closely related to poor prognosis. A growing number of studies have focused on the role of TAMs in BC progression and therapeutic strategies targeting TAMs. As an emerging treatment, the application of nanosized drug delivery systems (NDDSs) in the treatment of BC by targeting TAMs has attracted much attention. Aims This review is to summarize the characteristics and treatment strategies targeting TAMs in BC and to clarify the applications of NDDSs targeting TAMs in the treatment of BC by targeting TAMs. Materials & Methods The existing results related to characteristics of TAMs in BC, BC treatment strategies by targeting TAMs, and the applications of NDDSs in these strategies are described. Through analyzing these results, the advantages and disadvantages of the treatment strategies using NDDSs are discussed, which could provide advices on designing NDDSs for BC treatment. Results TAMs are one of the most prominent noncancer cell types in BC. TAMs not only promote angiogenesis, tumor growth and metastasis but also lead to therapeutic resistance and immunosuppression. Mainly four strategies have been used to target TAMs for BC therapy, which include depleting macrophages, blocking recruitment, reprogramming to attain an anti‐tumor phenotype, and increasing phagocytosis. Since NDDSs can efficiently deliver drugs to TAMs with low toxicity, they are promising approaches for targeting TAMs in tumor therapy. NDDSs with various structures can deliver immunotherapeutic agents and nucleic acid therapeutics to TAMs. In addition, NDDSs can realize combination therapies. Discussion TAMs play a critical role in the progression of BC. An increasing number of strategies have been proposed to regulate TAMs. Compared with free drugs, NDDSs targeting TAMs improve drug concentration, reduce toxicity and realize combination therapies. However, in order to achieve better therapeutic efficacy, there are still some disadvantages that need to be considered in the design of NDDSs. Conclusion TAMs play an important role in the progression of BC, and targeting TAMs is a promising strategy for BC therapy. In particular, NDDSs targeting TAMs have unique advantages and are potential treatments for BC.

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“…Zang et al constructed lipid-coated mannose-modified nanoparticles as carriers for calcium zoledronate, which reduced tumor angiogenesis and remodeled immunosuppressive TME [ 57 ]. Doxorubicin hydrochloride-loaded nanoparticles modified with zymosan (ChiNPs), developed by Pawar et al, could switch TAM polarization towards the M1 phenotype and reduce VEGFR2 expression in TME [ 58 ]. PLGA nanoparticles encapsulating melanoma antigen Hgp peptide and M2-targeting peptides on the surface successfully transformed M2-like TAMs to M1 phenotype, thus normalizing tumor angiogenesis and increasing CD8 + T cells and NK cell infiltration [ 59 ].…”

Section: Remodeling Tumor Angiogenesis Induced Hypoxic Microenvironmentmentioning

confidence: 99%

Firing up the Tumor Microenvironment with Nanoparticle-Based Therapies

et al. 2021

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Therapies mobilizing host immunity against cancer cells have profoundly improved prognosis of cancer patients. However, efficacy of immunotherapies depends on local immune conditions. The “cold” tumor, which is characterized by lacking inflamed T cells, is insensitive to immunotherapy. Current strategies of improving the “cold” tumor microenvironment are far from satisfying. Nanoparticle-based therapies provide novel inspiration in firing up the tumor microenvironment. In this review, we presented progress and limitations of conventional immunotherapies. Then, we enumerate advantages of nanoparticle-based therapies in remodeling the “cold” tumor microenvironment. Finally, we discuss the prospect of nanoparticle-based therapies in clinical application.

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Doxorubicin Hydrochloride Loaded Zymosan-Polyethylenimine Biopolymeric Nanoparticles for Dual ‘Chemoimmunotherapeutic’ Intervention in Breast Cancer

Cited by 14 publications

References 59 publications

Click Biotinylation of PLGA Template for Biotin Receptor Oriented Delivery of Doxorubicin Hydrochloride in 4T1 Cell-Induced Breast Cancer

Click Biotinylation of PLGA Template for Biotin Receptor Oriented Delivery of Doxorubicin Hydrochloride in 4T1 Cell-Induced Breast Cancer

Antitumor therapy for breast cancer: Focus on tumor‐associated macrophages and nanosized drug delivery systems

Firing up the Tumor Microenvironment with Nanoparticle-Based Therapies

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