Click Biotinylation of PLGA Template for Biotin Receptor Oriented Delivery of Doxorubicin Hydrochloride in 4T1 Cell-Induced Breast Cancer

Singh, Yuvraj; Viswanadham, K. K. Durga Rao; Jajoriya, Arun Kumar; Meher, Jayagopal; Raval, Kavit; Jaiswal, Swati; Dewangan, Jayant; Bora, Himangshu K.; Rath, Srikanta Kumar; Lal, Jawahar; Chourasia, Manish K.

doi:10.1021/acs.molpharmaceut.7b00310

Cited by 34 publications

(27 citation statements)

References 45 publications

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“…The transformation of lutein from crystalline to amorphous form is important, as amorphous forms are characterized by higher solubilities and increased bioavailability [ 51 , 52 , 53 , 54 ]. Similar results (loss of characteristic peak) were observed in other studies where crystalline drugs such as SN-38, doxorubicin oxcarbazepine, prilocaine, and adefovir were encapsulated into PLGA-based micro- and nanoparticles [ 24 , 25 , 55 , 56 , 57 ].…”

Section: Resultssupporting

confidence: 87%

“…Thus, it is known that biotinylated prodrugs and polymeric nanoparticles utilize the SMVT and biotin transporters for enhanced permeability of drugs [ 19 , 23 ]. Moreover, scientists have reported that biotin-decorated polymeric nanoparticles have enhanced the uptake of poorly soluble drugs such as doxorubicin [ 24 ], SN-38 [ 25 ], and 15,16-dihydrotanshinone [ 26 ]. Therefore, we hypothesize that lutein-loaded PLGA–PEG–biotin polymeric nanoparticles can enhance the uptake of lutein into the retinal cells through SMVT transport system.…”

Section: Introductionmentioning

confidence: 99%

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Lutein-Loaded, Biotin-Decorated Polymeric Nanoparticles Enhance Lutein Uptake in Retinal Cells

et al. 2020

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Age related macular degeneration (AMD) is one of the leading causes of visual loss and is responsible for approximately 9% of global blindness. It is a progressive eye disorder seen in elderly people (>65 years) mainly affecting the macula. Lutein, a carotenoid, is an antioxidant, and has shown neuroprotective properties in the retina. However, lutein has poor bioavailability owing to poor aqueous solubility. Drug delivery to the posterior segment of the eye is challenging due to the blood–retina barrier. Retinal pigment epithelium (RPE) expresses the sodium-dependent multivitamin transporter (SMVT) transport system which selectively uptakes biotin by active transport. In this study, we aimed to enhance lutein uptake into retinal cells using PLGA–PEG–biotin nanoparticles. Lutein loaded polymeric nanoparticles were prepared using O/W solvent-evaporation method. Particle size and zeta potential (ZP) were determined using Malvern Zetasizer. Other characterizations included differential scanning calorimetry, FTIR, and in-vitro release studies. In-vitro uptake and cytotoxicity studies were conducted in ARPE-19 cells using flow cytometry and confocal microscopy. Lutein was successfully encapsulated into PLGA and PLGA–PEG–biotin nanoparticles (<250 nm) with uniform size distribution and high ZP. The entrapment efficiency of lutein was ≈56% and ≈75% for lutein-loaded PLGA and PLGA–PEG–biotin nanoparticles, respectively. FTIR and DSC confirmed encapsulation of lutein into nanoparticles. Cellular uptake studies in ARPE-19 cells confirmed a higher uptake of lutein with PLGA–PEG–biotin nanoparticles compared to PLGA nanoparticles and lutein alone. In vitro cytotoxicity results confirmed that the nanoparticles were safe, effective, and non-toxic. Findings from this study suggest that lutein-loaded PLGA–PEG–biotin nanoparticles can be potentially used for treatment of AMD for higher lutein uptake.

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Section: Resultssupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Lutein-Loaded, Biotin-Decorated Polymeric Nanoparticles Enhance Lutein Uptake in Retinal Cells

et al. 2020

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“…This approach is widely used to antitumor agent design both in case when the targeting moiety is a low molecular weight compound (folate, carbohydrate residues, vitamins etc.) [147,194,254] or a protein (transferrin, growth factors) [131,255].…”

Section: Active Targetingmentioning

confidence: 99%

Targeted Delivery to Tumors: Multidirectional Strategies to Improve Treatment Efficiency

Kutova

Guryev

Соколова

et al. 2019

Cancers

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Malignant tumors are characterized by structural and molecular peculiarities providing a possibility to directionally deliver antitumor drugs with minimal impact on healthy tissues and reduced side effects. Newly formed blood vessels in malignant lesions exhibit chaotic growth, disordered structure, irregular shape and diameter, protrusions, and blind ends, resulting in immature vasculature; the newly formed lymphatic vessels also have aberrant structure. Structural features of the tumor vasculature determine relatively easy penetration of large molecules as well as nanometer-sized particles through a blood–tissue barrier and their accumulation in a tumor tissue. Also, malignant cells have altered molecular profile due to significant changes in tumor cell metabolism at every level from the genome to metabolome. Recently, the tumor interaction with cells of immune system becomes the focus of particular attention, that among others findings resulted in extensive study of cells with preferential tropism to tumor. In this review we summarize the information on the diversity of currently existing approaches to targeted drug delivery to tumor, including (i) passive targeting based on the specific features of tumor vasculature, (ii) active targeting which implies a specific binding of the antitumor agent with its molecular target, and (iii) cell-mediated tumor targeting.

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“…40 In the past decade, biotin was used as a targeting ligand for the delivery of various anti-cancer drugs, e.g., platinum, ferrocene, paclitaxel and doxorubicin, to tumors. 41–44…”

Section: Resultsmentioning

confidence: 99%

Fast functionalization of ultrasound microbubbles using strain promoted click chemistry

et al. 2018

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Functionalization of microbubbles (MBs) is a difficult issue due to their unstable nature. Here we report a fast and versatile method using a strain promoted alkyne-azide cycloaddition (SPAAC) click reaction for microbubble functionalization. An azadibenzocyclooctyne (DBCO) group was first introduced onto the MB surface and then an azide group into the desired ligand. Without any initiators or catalysts, essential click ligation occurred within 1 min and a majority of the reaction completed in 5 min at 37 °C. This fast ligation shortens the microbubble reaction time and preserves essential amounts of microbubbles for further in situ imaging and delivery of therapeutics.

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Click Biotinylation of PLGA Template for Biotin Receptor Oriented Delivery of Doxorubicin Hydrochloride in 4T1 Cell-Induced Breast Cancer

Cited by 34 publications

References 45 publications

Lutein-Loaded, Biotin-Decorated Polymeric Nanoparticles Enhance Lutein Uptake in Retinal Cells

Lutein-Loaded, Biotin-Decorated Polymeric Nanoparticles Enhance Lutein Uptake in Retinal Cells

Targeted Delivery to Tumors: Multidirectional Strategies to Improve Treatment Efficiency

Fast functionalization of ultrasound microbubbles using strain promoted click chemistry

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