Downstream Processing in Industrial Biotechnology

Hatti‐Kaul, Rajni

doi:10.1002/9783527630233.ch8

Cited by 10 publications

(7 citation statements)

References 54 publications

(46 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In fact, yeast cells density is only slightly higher than the reaction liquors in which they are suspended. Conventional solid-liquid separation methods include sedimentation, filtration or centrifugation; these methods tend to be time consuming and ⁄ or expensive, which prohibit their use in many industries (Hatti-Kaul and Mattiasson 2001). The sedimentation process requires large settling tanks and extensive retention times to obtain a cell-free supernatant.…”

Section: Industrial Application Of Yeast Flocculationmentioning

confidence: 99%

“…Separation of yeast cells from fermented medium can also be obtained by centrifugation; the two popular industrial centrifuges are disc stack and tubular bowl centrifuges (Shuler and Kargi 2002). Nevertheless, centrifugation can have a negative impact on yeast cells (see below ); in addition, this separation process is considered a relatively expensive method taking into account the costs of maintenance and power demand per quantity of micro‐organisms removed (Hatti‐Kaul and Mattiasson 2001). For example, the capital investments for centrifuges and the energy consumption for centrifuge operation hinder the use of this equipment in the production of bio‐ethanol (Xu et al.…”

Section: Industrial Application Of Yeast Flocculationmentioning

confidence: 99%

See 1 more Smart Citation

Flocculation in Saccharomyces cerevisiae: a review

Soares

2010

Journal of Applied Microbiology

246

201

View full text Add to dashboard Cite

Summary The present work reviews and critically discusses the aspects that influence yeast flocculation, namely the chemical characteristics of the medium (pH and the presence of bivalent ions), fermentation conditions (oxygen, sugars, growth temperature and ethanol concentration) and the expression of specific genes such as FLO1, Lg‐FLO1, FLO5, FLO8, FLO9 and FLO10. In addition, the metabolic control of loss and onset of flocculation is reviewed and updated. Flocculation has been traditionally used in brewing production as an easy and off‐cost cell‐broth separation process. The advantages of using flocculent yeast strains in the production of other alcoholic beverages (wine, cachaça and sparkling wine), in the production of renewal fuels (bio‐ethanol), in modern biotechnology (production of heterologous proteins) and in environmental applications (bioremediation of heavy metals) are highlighted. Finally, the possibility of aggregation of yeast cells in flocs, as an example of social behaviour (a communitarian strategy for long‐time survival or a means of protection against negative environmental conditions), is discussed.

show abstract

Section: Industrial Application Of Yeast Flocculationmentioning

confidence: 99%

Section: Industrial Application Of Yeast Flocculationmentioning

confidence: 99%

Flocculation in Saccharomyces cerevisiae: a review

Soares

2010

Journal of Applied Microbiology

246

201

View full text Add to dashboard Cite

show abstract

“…Small quantities of illudins have been recovered at laboratory scale either by solid phase or solvent extraction of culture broth [5,11,16,17]. Illudin M has additionally been reported to crystalize after concentration in a hexane/ethyl acetate fraction eluted from silica gel [11], which is desirable since crystalline material can usually be recovered with high purity depending on the crystallization method [18]. At industrial scale, soluble products obtained by submerged cultivation implicate large amounts of aqueous culture broth.…”

Section: Introductionmentioning

confidence: 99%

Optimization of the production process for the anticancer lead compound illudin M: downstream processing

2022

View full text Add to dashboard Cite

Background Secondary metabolites have played a key role as starting points for drug development programs due to their often unique features compared with synthetically derived molecules. However, limitations related to the discovery and supply of these molecules by biotechnological means led to the retraction of big pharmaceutical companies from this field. The reasons included problems associated with strain culturing, screening, re-discovery, purification and characterization of novel molecules from natural sources. Nevertheless, recent reports have described technical developments that tackle such issues. While many of these reports focus on the identification and characterization of such molecules to enable subsequent chemical synthesis, a biotechnological supply strategy is rarely reported. This may be because production processes usually fall under proprietary research and/or few processes may meet the requirements of a pharmaceutical development campaign. We aimed to bridge this gap for illudin M—a fungal sesquiterpene used for the development of anticancer agents—with the intention to show that biotechnology can be a vital alternative to synthetic processes dealing with small molecules. Results We used µL-scale models to develop an adsorption and extraction strategy for illudin M recovery from culture supernatant of Omphalotus nidiformis and these findings were successfully transferred into lab-scale. By adsorbing and eluting the product using a fixed resin-bed we reduced the working volume by ~ 90% and removed the aqueous phase from the process. After a washing step, a highly concentrated illudin M fraction was obtained by isocratic elution with 80% methanol. The fraction was dried and extracted using a water/heptane mixture, enriching illudin M in the heptane phase. From heptane illudin M could be instantly crystalized by concentrating the solution, achieving a final purity > 95%. Conclusion We have developed a robust, scalable and low-cost downstream process to obtain highly pure illudin M. By using solid phase extraction we reduced the production of solvent waste. Heptane from the final purification step could be recycled. The reduced amounts of solvents required, and the short purification time render this method a very economic and ecologic alternative to published processes.

show abstract

“…Peroxidase can be extracted from the plants and animals or produced by microorganisms in the fermenter 17,18 . The enzyme extraction from the plant sources has considerable advantages including low production cost and renewability 19 . Up to now, peroxidase has been extracted from different plants such as Brassica rapa, Lycopersicon Esculentum, Raphanus Sativus L., Brassica oleracea [20][21][22][23] .…”

Section: Introductionmentioning

confidence: 99%

A novel method for production of peroxidase enzyme from garden radish root source using a spouted bed reactor modeling and optimization approach

Hamedi

Afsahi

Riahi‐Madvar

2021

Preprint

View full text Add to dashboard Cite

The main advantages of the dried enzymes are the lower cost of storage and longer time of preservation for industrial applications. In this study for the first time, the spouted bed reactors were utilized for drying the garden radish (Raphanus sativus L.) root extract as a cost-effective source of the peroxidase enzyme. The response surface methodology (RSM) was used to evaluate the individual and interactive effects of main parameters (the inlet air temperature (T) and the air flow rate (Q)) on the residual enzyme activity (REA). The maximum REA of 38.7% was obtained at the T= 50 °C and Q= 1.4. In order to investigate the drying effect on the catalytic activity, the optimum reaction conditions (pH and temperature), as well as kinetic parameters, were investigated for the fresh and dried enzyme extracts (FEE and DEE). The obtained results showed that optimum pH was decreased about 12.3% while 85.7% increase was observed in optimum temperature of DEE compare to FEE. Moreover, kinetic parameters, thermal-stability, and shelf life of the enzyme were considerably improved after drying by the spouted bed. Overall, the results confirmed that a spouted bed reactor can be used as a promising method for drying heat-sensitive materials such as peroxidase enzyme.

show abstract

Downstream Processing in Industrial Biotechnology

Cited by 10 publications

References 54 publications

Flocculation in Saccharomyces cerevisiae: a review

Flocculation in Saccharomyces cerevisiae: a review

Optimization of the production process for the anticancer lead compound illudin M: downstream processing

A novel method for production of peroxidase enzyme from garden radish root source using a spouted bed reactor modeling and optimization approach

Contact Info

Product

Resources

About