Downstream processing and characterization of pullulan from a novel colour variant strain of Aureobasidium pullulans FB-1

Singh, Ram Sarup; Saini, Gaganpreet K.; Kennedy, John F.

doi:10.1016/j.carbpol.2009.03.040

Cited by 69 publications

(13 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The confirmation of structural characteristics of pullulan by 13 C NMR spectroscopy has been found similar with Youssef et al [61]. Our results corroborate the findings on pullulan production from A. pullulans [62].…”

Section: Nmr Spectroscopy Of Crude Pullulansupporting

confidence: 92%

Biochemical and molecular characterization of a new pullulan producer Rhodosporidium paludigenum PUPY-06

2018

J App Biol Biotech

View full text Add to dashboard Cite

Pullulan is a random coil glucan polymer produced by Aureobasidium pullulans. It is one of the commercially promising biopolymers frequently used in pharmaceutical, food and cosmetics industries. The aim of present study was to isolate an efficient fungal strain producing melanin-free pullulan. The fungal strains were isolated from fresh and decaying leaves of plants and screened for pullulan production. Fungal isolate PUPY-06 was the best producer of pullulan (2.12%, w/v). Morphological studies revealed the oval and cylindrical shape of its cells, budding and presence of septate as well as pseudohyphae. Colonies of the isolate on agar plate cultures were beige-to-orange colored with smooth, mucoid, and butyrous texture. Morphological and biochemical characterization established the fungal isolate as Rhodosporidium sp. 18s rRNA sequencing, and phylogenetic analysis revealed the isolate to be Rhodosporidium paludigenum. Fourier-transform infrared spectroscopy and nuclear magnetic resonance of crude pullulan confirmed its structural characteristics. Maximum production of pullulan was achieved after 7 days of cultivation in shake-flask fermentations. Agitation mode of cultivation supported the higher production of pullulan in comparison to the stationary mode. R. paludigenum PUPY-06 was found as a novel pullulan-producing strain. This is a first report on pullulan production by R. paludigenum PUPY-06.

show abstract

Section: Nmr Spectroscopy Of Crude Pullulansupporting

confidence: 92%

Biochemical and molecular characterization of a new pullulan producer Rhodosporidium paludigenum PUPY-06

2018

J App Biol Biotech

View full text Add to dashboard Cite

show abstract

“…Melanin is one of the major problems in pullulan production and is responsible for dark green to black colour of the broth. Thus, an appropriate downstream processing of the fermentation broth is required to alleviate the pigmentation problem for melanin producing strains (Singh, Saini & Kennedy, 2009). With this new strain the fermentation broth turned to dark green only after 7 days of incubation or a medium addition of CSL.…”

Section: Pullulansmentioning

confidence: 99%

Extracellular polysaccharide (EPS) production by a novel strain of yeast-like fungus Aureobasidium pullulans

Rao¹,

Quiñones

Retter³

et al. 2010

Carbohydrate Polymers

View full text Add to dashboard Cite

“…Pullulan was produced from A. pullulans FB-1 in shake-flask fermentations as reported earlier and purified by the method as described by current authors (Singh, Saini, & Kennedy, 2009). Purified pullulan was used for hydrolysis in continuous system.…”

Section: Substratementioning

confidence: 99%

Continuous hydrolysis of pullulan using covalently immobilized pullulanase in a packed bed reactor

Singh

Saini

Kennedy³

2011

Carbohydrate Polymers

Self Cite

View full text Add to dashboard Cite

Downstream processing and characterization of pullulan from a novel colour variant strain of Aureobasidium pullulans FB-1

Cited by 69 publications

References 28 publications

Biochemical and molecular characterization of a new pullulan producer Rhodosporidium paludigenum PUPY-06

Biochemical and molecular characterization of a new pullulan producer Rhodosporidium paludigenum PUPY-06

Extracellular polysaccharide (EPS) production by a novel strain of yeast-like fungus Aureobasidium pullulans

Continuous hydrolysis of pullulan using covalently immobilized pullulanase in a packed bed reactor

Contact Info

Product

Resources

About