Downregulation of the long non-coding RNA ZFAS1 is associated with cell proliferation, migration and invasion in breast cancer

Fan, Shulin; Fan, Chi; Liu, Ning; Huang, Keke; Fang, Xuedong; Wang, Keren

doi:10.3892/mmr.2018.8707

Cited by 43 publications

(57 citation statements)

References 27 publications

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“…Unexpectedly, the expression level of ZFAS1 in a portion in NPC specimens was lower than the average expression level of para-carcinoma specimens. 39 Here, we found that the high-expression of ZFAS1 was significantly associ- Here, we found that ZFAS1 could indirectly regulate the expression of downstream LPAR1 in a miR-892b-dependent manner. Overexpression of ZFAS1 has been found to significantly promote the proliferation and inhibit the apoptosis of gastric cancer cells by repressing KLF2 and NKD2 expression.…”

Section: Discussionmentioning

confidence: 71%

Long noncoding RNA ZFAS1 promotes tumorigenesis and metastasis in nasopharyngeal carcinoma by sponging miR‐892b to up‐regulate LPAR1 expression

Peng

Liu

Zheng

et al. 2019

J Cellular Molecular Medi

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Objective: In this study, we explored the NPC-specific expression of ZFAS1 and the mechanism of ZFAS1-mediated growth, aggressiveness and tumorigenesis in NPC. Methods:The expression profile of lncRNAs was detected in NPC tissues and matching para-carcinoma tissues using microarray analysis. LncRNA-miRNA and miRNA-mRNA interaction networks were constructed using the miRcode v11 and TargetScanHuman v7.2 web server and then validated using dual-luciferase assay.Western blot and RT-qPCR were performed to detect protein and RNA expression.The effects of ZFAS1, miR-892b and LPAR1 dysregulation on the proliferative, migratory and invasive abilities of NPC cells were observed using colony formation, cell counting kit-8 (CCK-8) and transwell assays in vitro. In vivo, a xenograft nude mouse model was established to detect the impact of ZFAS1 dysregulation on the tumorigenicity of NPC cells. Results:The expression of multiple lncRNAs, of which ZFAS1 was up-regulated, was dysregulated in NPC tissues. ZFAS1 directly targeted miR-892b, and miR-892b negatively regulated the expression of downstream LPAR1. The proliferation, migration and invasion of NPC cells could be largely enhanced by the downregulation of miR-892b as well as the up-regulation of ZFAS1 and LPAR1, while the overexpression of miR-892b and the downregulation of ZFAS1 and LPAR1 decreased these abilities.In nude mice, the growth of tumour xenografts formed by HONE1 cells was significantly suppressed when ZFAS1 was silenced. Conclusion:The study demonstrated that lncRNA ZFAS1 may act as a promoter of tumorigenesis and metastasis in nasopharyngeal carcinoma, by up-regulating the expression of LPAR1 in a miR-892b-dependent manner. K E Y W O R D Slong noncoding RNAs, LPAR1, miR-892b, nasopharyngeal carcinoma, ZFAS1

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Section: Discussionmentioning

confidence: 71%

Long noncoding RNA ZFAS1 promotes tumorigenesis and metastasis in nasopharyngeal carcinoma by sponging miR‐892b to up‐regulate LPAR1 expression

Peng

Liu

Zheng

et al. 2019

J Cellular Molecular Medi

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“…For example, LINC01446 were found to promote glioma cell proliferation and invasion, 6 or lncRNA NEAT1 could promote the radioresistance of gastric cancer cells 7 . ZFAS1 has also been proved to promote proliferation, migration, and invasion of breast cancer cells 8 . The important function of lncRNAs has been widely accepted and some lncRNAs have even been regarded as prognostic factors of cancers.…”

Section: Introductionmentioning

confidence: 99%

Long noncoding RNA PRKCQ‐AS1 promotes CRC cell proliferation and migration via modulating miR‐1287‐5p/YBX1 axis

Cui

Zhao

Li³

2020

J of Cellular Biochemistry

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Colorectal cancer (CRC) brings more than 600 000 deaths every year around the globe, making itself the third most frequently occurred carcinoma. The great progress human achieved in diagnosis and treatment of various cancers has failed to reverse this trend. Fortunately, growing evidence has implied the relationship between lncRNAs and cancer progression. Long noncoding RNA (lncRNA) PRKCQ‐AS1 was heightened in CRC cells and tissues and related with dismal prognosis of CRC patients. Knockdown of PRKCQ‐AS1 would induce a decrease in proliferative and migrating ability of CRC cells. Also, PRKCQ‐AS1 enriched in cytoplasm of CRC cells and negatively regulated miR‐1287‐5p level. More important, PRKCQ‐AS1 could bind to argonaute 2 and function in the RNA‐induced silencing complex with miR‐1287‐5p. Therefore, PRKCQ‐AS1 was a competing endogenous RNA for miR‐1287‐5p. Subsequently, it was validated that miR‐1287‐5p could suppress the proliferative and migratory functions in CRC. Furthermore, PRKCQ‐AS1 could upregulate the mRNA and protein level of YBX1 targeted by miR‐1287‐5p. And YBX1 expression was elevated in CRC cells and tissues. Rescue assays in vitro and in vivo showed that overexpression of YBX1 could partly offset the effect of CRC progression induced by knocking down PRKCQ‐AS1, demonstrating PRKCQ‐AS1 mediating CRC progression via miR‐1287‐5p/YBX1 pathway.

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“…Breast cancer is the most commonly diagnosed malignancy and one of the leading causes of cancer-associated death in women worldwide (1)(2)(3)(4)(5). Breast tumors are heterogeneous neoplasms, composed of multiple subtypes, which exhibit distinct morphologies and clinical features (6,7).…”

Section: Introductionmentioning

confidence: 99%

“…Breast tumors are heterogeneous neoplasms, composed of multiple subtypes, which exhibit distinct morphologies and clinical features (6,7). Clinically, although great advances have been made in the treatment of breast cancer over the last decade, recurrence and metastasis remain the principal causes of mortality in patients with this disease (2,(8)(9)(10)(11). Therefore, elucidating the pathogenic mechanisms underlying breast cancer development and progression to identify prognostic biomarkers may provide potentially novel therapeutic targets.…”

Section: Introductionmentioning

confidence: 99%

Linc‑OIP5 loss regulates migration and invasion in MDA‑MB‑231 breast cancer cells by inhibiting YAP1/JAG1 signaling

Zhu

Dong

et al. 2019

Oncol Lett

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Breast cancer is the most prevalent cancer among women, and diagnosis and treatment represent a substantial challenge due to the lack of adequate molecular targets. It has been shown that long noncoding RNAs (lncRNAs) serve pivotal roles in regulating gene expression in tumors. The roles of long intervening noncoding RNA (Linc)-OIP5 has been demonstrated in different types of cancer; however, its function in breast cancer has not been determined. In the present study, expression of Linc-OIP5, YAP1 (Hippo signaling component) and JAG1 (Notch signaling component) in breast cancer cells with different degrees of malignancy were determined. To assess whether Linc-OIP5 regulated the malignant biological behaviors of MDA-MB-231 cells, its expression was knocked down using a specific small interfering RNA (siRNA), and cell proliferation was determined using a CCK-8 assay, apoptosis was evaluated using an Annexin V-FITC apoptosis detection kit, migration was assessed using a wound healing and transwell migration assays, and cell invasion examined using a transwell invasion assays. The effect of Linc-OIP5 knockdown on YAP1 and JAG1 expression was quantified using reverse transcription-quantitative PCR and immunoblotting. Cell proliferation, migration and invasion were reduced, while apoptosis was increased in MDA-MB-231 cells transfected with Linc-OIP5-specific siRNA. Mechanistic investigations showed that Linc-OIP5 knockdown downregulated YAP1 and JAG1 expression. The results of the present study suggest that Linc-OIP5 affects the malignant biological behaviors of MDA-MB-231 cells, at least partly through its effects on YAP1/JAG1 signaling. Whilst there are a number of mechanisms underlying the pathogenesis of breast cancer, the results of the present study highlight Linc-OIP5 as a potential therapeutic target in breast cancer.

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Downregulation of the long non-coding RNA ZFAS1 is associated with cell proliferation, migration and invasion in breast cancer

Cited by 43 publications

References 27 publications

Long noncoding RNA ZFAS1 promotes tumorigenesis and metastasis in nasopharyngeal carcinoma by sponging miR‐892b to up‐regulate LPAR1 expression

Long noncoding RNA ZFAS1 promotes tumorigenesis and metastasis in nasopharyngeal carcinoma by sponging miR‐892b to up‐regulate LPAR1 expression

Long noncoding RNA PRKCQ‐AS1 promotes CRC cell proliferation and migration via modulating miR‐1287‐5p/YBX1 axis

Linc‑OIP5 loss regulates migration and invasion in MDA‑MB‑231 breast cancer cells by inhibiting YAP1/JAG1 signaling

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