Downregulation of long non-coding RNA TRIM52-AS1 functions as a tumor suppressor in renal cell carcinoma

Liu, Zan; Yan, Haiyan; Xia, Shunyao; Zhang, Cheng; Xiu, Youcheng

doi:10.3892/mmr.2016.4908

Cited by 27 publications

(24 citation statements)

References 31 publications

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“…Using WGCNA and hypergeometric test analysis, we found eight lncRNAs with important functions: AL671710.1, TRIM52-AS1, C1orf220, AC022762.2, DANCR, LINC01569, AC084018.1 and MIR194-2HG. TRIM52-AS1 is one of the eight lncRNAs that has been reported as a function of a tumor suppressor (Liu et al 2016;Zhang et al 2017).Targeted by MYC, DANCR promotes cancer (Chen et al 2016b;Dhanasekaran et al 2017;Huang et al 2013;Kron et al 2012;Lu et al 2018b). Taken together, we concluded that these lncRNAs may function as a potential tumor regulator in HCC.…”

Section: Discussionsupporting

confidence: 53%

Peer Review #3 of "Co-expression analysis and ceRNA network reveal eight novel potential lncRNA biomarkers in hepatocellular carcinoma (v0.1)"

Merza¹

2019

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Background: Hepatocellular carcinoma (HCC) is the most common primary liver cancer in the world, with a high degree of malignancy and recurrence. The influence of ceRNA network in tumor on the biological function of liver cancer is very important, It has been reported that many lncRNA play key role in liver cancer development. In our study, integrated data analysis revealed potential eight novel lncRNA biomarkers in hepatocellular carcinoma. Methods: Transcriptome data and clinical data were downloaded from the The Cancer Genome Atlas (TCGA) data portal.Weighted gene coexpression network analysis was performed to identify the expression pattern of genes in liver cancer. Then ceRNA network was constructed using transcriptome data. Results: The integrated analysis of miRNA and RNAseq in the database show eight novel lncRNAs that may be involved in important biological pathways including TNM and disease development in liver cancer. We performed function enrichment analysis about mRNAs affected by these lncRNAs. Conclusions: By identifying the ceRNA network and lncRNAs that affect liver cancer, we showed that eight novel lncRNAs played an important role in the development and progress of liver cancer.

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Section: Discussionsupporting

confidence: 53%

Peer Review #3 of "Co-expression analysis and ceRNA network reveal eight novel potential lncRNA biomarkers in hepatocellular carcinoma (v0.1)"

Merza¹

2019

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“…Long non-coding RNA (lncRNA), at a length of >200 nucleotides, is identified as endogenous cellular RNAs lack of open reading frames of significant length (<100 amino acids) (6,8). With the development of gene sequencing, more and more lncRNAs have been identified (9); however, the functions of most lncRNAs remain unclear. Previous studies have revealed that certain lncRNAs may regulate gene expression at various levels and serve important roles in tumorigenesis and development (10).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of long non-coding RNA differentiation antagonizing non-protein coding RNA inhibits the proliferation, migration and invasion and promotes apoptosis of renal cell carcinoma

Jin

Fu²,

Quan

et al. 2017

Molecular Medicine Reports

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Renal cell carcinoma (RCC) is the third most common cancer in the urological system; however, the pathogenesis remains unknown. Accumulating evidence has demonstrated that long non‑coding RNAs are dysregulated in various tumors and serves an important role in tumorigenesis and development. In the present study, expression of lncRNA differentiation antagonizing non‑protein coding RNA (DANCR) in 24 paired RCC and adjacent normal tissues was detected by reverse transcription‑quantitative polymerase chain reaction. The results revealed that DANCR is downregulated in RCC tissues compared with adjacent normal tissues. Subsequent study revealed that overexpression of lncRNA DANCR by transfection of pcDNA3.1‑DANCR could suppress 786‑O and ACHN (RCC cell) proliferation, migration and invasion, and induce apoptosis compared with cells transfected with the pcDNA3.1 vector. The results revealed that DANCR functions as a tumor suppressor in RCC. In conclusion, the present study, to the best of our knowledge, was the first to reveal DANCR as a tumor suppressor. The results implicate DANCR as a potential biomarker of RCC, and further study will be focused on the clinical significance and signaling pathways of DANCR.

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“…As non-coding RNA molecules in cells, lncRNAs participate in the regulation of multiple cell functions, such as cell proliferation, cell differentiation, cell migration and cell apoptosis [9]. Moreover, studies also demonstrate that lncRNAs play critical regulatory roles in a variety of disease processes, including neuro-degeneration and carcinogenesis [10,11] LncRNA urothelial carcinoma associated 1 (UCA1) has been aroused more and more attention in recent years due to its oncogenic/ tumor-suppressive roles in numerous cancer cells [12,13].…”

Section: Introductionmentioning

confidence: 99%

Long Non-Coding RNA Urothelial Carcinoma Associated 1 Promotes Proliferation, Migration and Invasion of Osteosarcoma Cells by Regulating microRNA-182

Xing

Gong

et al. 2018

Cell Physiol Biochem

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Background/Aims: Previous studies demonstrated the oncogenic roles of lncRNA UCA1 in osteosarcoma. This study aimed to explore the internal molecular mechanism of UCA1 on promoting osteosarcoma cell proliferation, migration and invasion. Methods: qRT-PCR was conducted to measure the expression levels of UCA1, miR-182 and TIMP2. Cell transfection was used to change the expression levels of UCA1, miR-182 and TIMP2. Cell viability, migration, invasion and apoptosis were measured using CCK-8 assay, two-chamber migration (invasion) assay and Guava Nexin assay, respectively. The associations between UCA1, miR-182 and iASPP were analyzed by dual luciferase activity assay. The protein expression levels of key factors involved in cell apoptosis, PI3K/AKT/GSK3β pathway and NF-κB pathway, as well as p53, Rb, RECQ family and iASPP were evaluated by western blotting. Results: UCA1 was highly expressed in osteosarcoma MG63 and OS-732 cells. Knockdown of UCA1 inhibited OS-732 cell viability, migration and invasion, but promoted cell apoptosis. miR-182 was up-regulated in OS-732 cells after UCA1 knockdown and participated in the effects of UCA1 on OS-732 cells. TIMP2 was downstream factor of miR-182 and involved in the regulatory roles of miR-182 on OS-732 cell viability, migration, invasion, apoptosis, as well as PI3K/AKT/GSK3β and NF-κB pathways. UCA1 knockdown up-regulated p53, Rb and RECQL5 levels in OS-732 cells, while down-regulated the expression of iASPP. TGF-β or TNF-α treatment could enhance the expression of UCA1 in OS-732 cells. Conclusion: Our research verified that UCA1 exerted oncogenic roles in osteosarcoma cells by regulating miR-182 and TIMP2, as well as PI3K/AKT/GSK3β and NF-κB pathways.

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Downregulation of long non-coding RNA TRIM52-AS1 functions as a tumor suppressor in renal cell carcinoma

Cited by 27 publications

References 31 publications

Peer Review #3 of "Co-expression analysis and ceRNA network reveal eight novel potential lncRNA biomarkers in hepatocellular carcinoma (v0.1)"

Peer Review #3 of "Co-expression analysis and ceRNA network reveal eight novel potential lncRNA biomarkers in hepatocellular carcinoma (v0.1)"

Overexpression of long non-coding RNA differentiation antagonizing non-protein coding RNA inhibits the proliferation, migration and invasion and promotes apoptosis of renal cell carcinoma

Long Non-Coding RNA Urothelial Carcinoma Associated 1 Promotes Proliferation, Migration and Invasion of Osteosarcoma Cells by Regulating microRNA-182

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