Downregulation of iNOS expression in rat mesangial cells by special extracts of Harpagophytum procumbens derives from harpagoside-dependent and independent effects

Kaszkin, Marietta; Beck, Karl‐Friedrich; Koch, Eckhard; Erdelmeier, C. A. J.; Kusch, Stefan; Pfeilschifter, Josef; Loew, D.

doi:10.1016/j.phymed.2004.02.003

Cited by 65 publications

(49 citation statements)

References 33 publications

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“…Although no significant treatment effect was observed for stimulated Cys-LT levels, a biphasic decrease in basal Cys-LT was established with a 28% reduction after 4 h and a 58% reduction after 8 h. Pharmacokinetic investigations revealed that maximum levels of harpagoside were attained after 1.3 to 2.5 h and a correlation was determined between serum harpagoside levels and LT inhibition. The relevance of basal Kaszkin et al, 2004Huang et al, 2005Langmead et al, 2002BetancorFernandez et al, 2003Fiebich et al, 2001Schulze-Tanzil et al, 2004;Goldring and Goldring, 2004Moussard et al, 1992Loew et al, 2001 Results No analgesic effect as measured by prostaglandin synthetase activity Antiinflammatory effect through suppression of biosynthesis of cysteinyl-leukotrienes (Cys-LT; LTC 4 , LTD 4 and LTE 4 ) and TXB 2 Antiinflammatory effect by inhibition of TXB 2 by platelets Direct inhibitory effect on the COX-2 enzyme Analgesic effect due to significant reduction in expression of PGE 2 Concentration-dependent suppressions in nitrite release and iNOS expression Significant inhibition of COX-2 and iNOS Antioxidant abilities Antiinflammatory effect through inhibition of proinflammatory cytokines Eicosanoid production not altered via the COX or 5-LOX pathways in healthy individuals Effect on eicosanoid biosynthesis implied by biphasic decrease in basal Cys-LT in healthy men eicosanoid levels to active inflammatory conditions rather than those released following stimuli, could be questioned, however. As found in the animal studies discussed previously, the apparent discrepancies in results yielded from these eicosanoid investigations could more than likely be attributed to the fact that standardized extracts, with a universal harpagoside content, were not used in every case.…”

Section: Preclinical Research (In Vitro and Ex Vivo)mentioning

confidence: 99%

A review of the biological and potential therapeutic actions of Harpagophytum procumbens

Grant

McBean

Fyfe

et al. 2007

Phytotherapy Research

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Harpagophytum procumbens (Hp), commonly known as Devil's Claw is a perennial plant which thrives in arid conditions. For centuries, it has been used as a traditional treatment for a variety of illnesses, including fevers, skin complaints, arthritis and diseases of the digestive tract as well as an appetite stimulant. Since its introduction to Europe in the early twentieth century, it has become a popular antiinflammatory and analgesic preparation amongst herbalists for supportive or adjuvant treatment of degenerative joint diseases, tendonitis, headache, backache and menstrual pain. The validity of Hp as an effective antiinflammatory and analgesic preparation, particularly in the relief of arthritic symptoms, has been investigated in numerous animal, clinical and in vitro studies. Although some contradictory evidence exists, the majority of animal studies appear to indicate Hp as an effective antiinflammatory and analgesic preparation in the treatment of acute and subacute inflammation. Clinical trials support Hp as a beneficial treatment for the alleviation of pain and improvement of mobility in a variety of musculoskeletal conditions. Analysis of the in vitro and ex vivo studies that currently exist, indicate that Hp has significant effects on numerous proinflammatory markers. However, the exact mechanism(s) by which Hp may reduce inflammation remain to be elucidated.

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Section: Preclinical Research (In Vitro and Ex Vivo)mentioning

confidence: 99%

A review of the biological and potential therapeutic actions of Harpagophytum procumbens

Grant

McBean

Fyfe

et al. 2007

Phytotherapy Research

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“…Using the reverse transcriptionalpolymerase chain reaction, Jang et al (2003) found that an aqueous extract of Hp caused a significant dosedependent (100-1000 μg/mL) inhibition of LPS-induced iNOS expression in L929 murine cells. Similarly, Kaszkin et al (2004) demonstrated significant concentrationdependent (0.3-1 mg/mL) suppressions in nitrite release and iNOS expression from Hp-treated IL-1β-stimulated rat mesangial cells. It was further demonstrated that Hp treatment inhibited the translocation of NF-κB from the nucleus which most probably explains the suppression of iNOS and thus nitrite release.…”

Section: Discussionmentioning

confidence: 84%

The Inhibition of Free Radical Generation by Preparations of Harpagophytum procumbens in vitro

Grant

McBean

Fyfe

et al. 2008

Phytotherapy Research

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Harpagophytum procumbens (Hp), commonly known as Devil's Claw has been used as a traditional treatment for a variety of illnesses for centuries. Since the early twentieth century, it has become a popular antiinflammatory and analgesic preparation amongst European herbalists for supportive or adjuvant treatment of degenerative joint diseases. Extracts of Hp tubers have demonstrated antiinflammatory and analgesic effects in animal models of inflammation and in human trials. The mechanism(s) of action responsible for these attributes, however, remain to be elucidated. Reactive oxygen species generated in acute and chronic inflammatory diseases are known to be cytotoxic and can cause tissue damage. In this study, a root tuber extract (Hp extract) and commercially available tincture (Hp tincture) were investigated for antioxidant characteristics using in vitro test systems. Both preparations were found to effectively scavenge DPPH radical, inhibit nitrite levels in supernatants harvested from LPS-stimulated RAW 264.7 macrophages, and cause dose-dependent suppressions in the detection of fMLP-and AA-induced neutrophil MPO. The antioxidant effects demonstrated for both preparations of Hp may contribute to the antiinflammatory and analgesic properties observed for the plant.

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“…Harpagoside inhibited in vitro the synthesis of various proinflammatory mediators via suppression of iNOS and COX-2 expression through inhibition of NF-kappa B activation [61], but showed no anti-inflammatory activity in one animal model of inflammation (carrageenan-induced paw edema) [55]. The scientists suggested that other phytochemicals than harpagoside may be contributors to the anti-inflammatory effect of HP [55,62].…”

Section: Harpagophytum Procumbens Fam Pedaliaceaementioning

confidence: 99%

Phytomedicine in Joint Disorders

Dragoș

Gîlcă

Găman

et al. 2017

Preprint

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Chronic joint inflammatory disorders such as osteoarthritis and rheumatoid arthritis have in common an upsurge of inflammation, and oxidative stress, resulting in progressive histological alterations and disabling symptoms. Currently used conventional medication (ranging from painkillers to biological agents) is potent, but frequently associated with serious, even life-threatening side effects. Used for millennia in traditional herbalism, medicinal plants are a promising alternative, with lower rate of adverse events and an efficiency frequently comparable with that of conventional drugs. Nevertheless, their mechanism of action is in many cases elusive and/or uncertain. Even many of them have been proved effective in studies done in vitro or on animal models, there is a scarcity of human clinical evidence. The purpose of this review is to summarise the available scientific information on these joint-friendly medicinal plants, which have been already tested in human studies: Arnica montana, Boswellia spp., Curcuma spp., Equisetum arvense, Harpagophytum procumbens, Salix spp., Sesamum indicum, Symphytum officinalis, Zingiber officinalis, Panax notoginseng, Whitania somnifera.

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Downregulation of iNOS expression in rat mesangial cells by special extracts of Harpagophytum procumbens derives from harpagoside-dependent and independent effects

Cited by 65 publications

References 33 publications

A review of the biological and potential therapeutic actions of Harpagophytum procumbens

A review of the biological and potential therapeutic actions of Harpagophytum procumbens

The Inhibition of Free Radical Generation by Preparations of Harpagophytum procumbens in vitro

Phytomedicine in Joint Disorders

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