Downregulated Lipid Metabolism in Differentiated Murine Adipocytes by Procyanidins from Defatted Grape Seed Meal

Kim, Young-Hwa; Choi, Yearn–Ik; Lee, Junsoo; Park, Yooheon

doi:10.1271/bbb.130048

Cited by 9 publications

(4 citation statements)

References 38 publications

(38 reference statements)

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“…These secondary metabolites, especially flavonoids, play multiple roles in grape and attract more and more attentions due to their health benefits (Anastasiadi et al, 2010; Kim et al, 2013; Zhang et al, 2013). During grape berry development, the detected flavonoids presented two distinct changing patterns (Figure 2E).…”

Section: Resultsmentioning

confidence: 99%

“…Grape berries are consumed either as fresh fruit or processed to raisins, juice and wine. Besides its enormous economical and nutritional values, grapes and grape products possess a wide variety of health benefits, such as antioxidation (Sánchez-Moreno et al, 1999; Doshi et al, 2006; Sánchez-Alonso et al, 2007; Sáyago-Ayerdi et al, 2009; Anastasiadi et al, 2010), cardiovascular protection (Tebib et al, 1994; Adisakwattana et al, 2010; Razavi et al, 2013), neuroprotection (Feng et al, 2007), anti-obesity (Kim et al, 2013; Zhang et al, 2013), etc.…”

Section: Introductionmentioning

confidence: 99%

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System-Level and Granger Network Analysis of Integrated Proteomic and Metabolomic Dynamics Identifies Key Points of Grape Berry Development at the Interface of Primary and Secondary Metabolism

et al. 2017

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Grapevine is a fruit crop with worldwide economic importance. The grape berry undergoes complex biochemical changes from fruit set until ripening. This ripening process and production processes define the wine quality. Thus, a thorough understanding of berry ripening is crucial for the prediction of wine quality. For a systemic analysis of grape berry development we applied mass spectrometry based platforms to analyse the metabolome and proteome of Early Campbell at 12 stages covering major developmental phases. Primary metabolites involved in central carbon metabolism, such as sugars, organic acids and amino acids together with various bioactive secondary metabolites like flavonols, flavan-3-ols and anthocyanins were annotated and quantified. At the same time, the proteomic analysis revealed the protein dynamics of the developing grape berries. Multivariate statistical analysis of the integrated metabolomic and proteomic dataset revealed the growth trajectory and corresponding metabolites and proteins contributing most to the specific developmental process. K-means clustering analysis revealed 12 highly specific clusters of co-regulated metabolites and proteins. Granger causality network analysis allowed for the identification of time-shift correlations between metabolite-metabolite, protein- protein and protein-metabolite pairs which is especially interesting for the understanding of developmental processes. The integration of metabolite and protein dynamics with their corresponding biochemical pathways revealed an energy-linked metabolism before veraison with high abundances of amino acids and accumulation of organic acids, followed by protein and secondary metabolite synthesis. Anthocyanins were strongly accumulated after veraison whereas other flavonoids were in higher abundance at early developmental stages and decreased during the grape berry developmental processes. A comparison of the anthocyanin profile of Early Campbell to other cultivars revealed similarities to Concord grape and indicates the strong effect of genetic background on metabolic partitioning in primary and secondary metabolism.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

System-Level and Granger Network Analysis of Integrated Proteomic and Metabolomic Dynamics Identifies Key Points of Grape Berry Development at the Interface of Primary and Secondary Metabolism

et al. 2017

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“…The anti-obesity potential of oligomeric and polymeric procyanidin fractions from grape seeds was assessed in 3T3-L1 adipoyctes. Treatment resulted in lowered lipid accumulation, but not by lipolytic enzyme activity [224].…”

Section: From Seedsmentioning

confidence: 83%

Obesity: The Metabolic Disease, Advances on Drug Discovery and Natural Product Research

Castro¹,

Preto²,

Vasconcelos³

et al. 2016

CTMC

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Obesity is a global health threat. OECD reported that more than half (52%) of the adult population in the European Union is overweight or obese. Obesity and obesity-related co-morbidities have deep negative effects on morbidity, mortality, professional and personal quality of life. Healthcare costs represent a negative impact of this disease, with an associated economic cost of 100 billion US$ per year in the United States. The most prescribed drugs for obesity treatment worldwide are orlistat, and phentermine/topiramate extended release, while the major prescribed drug for the same disease in the US are exenatide and dapagliflozin. The so far developed drugs, targeting weight loss, have a long history of malignant secondary effects. There is still a lack of efficient and safe drugs to treat obesity and related metabolic complications since in many cases cure cannot be reached by bariatric surgery or healthy lifestyle habits. Terrestrial and aquatic organisms are a promising source of valuable, bioactive compounds, often with interest for human health. Some of the natural compounds or organisms have been used for centuries by humans as traditional medicine foods. In this review, we give insights into the adipose tissue function and development, and the progress in traditional anti-obesity pharmacotherapy. A major focus is to highlight the state of the art of natural compounds with anti-obesity properties and their potential as candidates for drug development; an overview is given about natural compounds derived from different marine animal sources, cyanobacteria, marine phytoplankton, fungus or plants.

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“…Grape seed procyanidins (GSP) are reported as natural antioxidants and are widely used for their free radical scavenging activity (Iglesias, Pazos, Torres, & Medina, ). Furthermore, GSP improves lipid metabolism, limits adipogenesis, enhances the immune system function, and ameliorates atherosclerosis (Kim, Choi, Lee, & Park, ; Pinent et al., ; Qi et al., ; Tong, Song, Sun, Sun, & Du, ).…”

Section: Introductionmentioning

confidence: 99%

A Mixture of Functional Complex Extracts from Lycium barbarum and Grape Seed Enhances Immunity Synergistically In Vitro and In Vivo

You

Chang

Zhao

et al. 2019

Journal of Food Science

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A mixture of multiple ingredients is often more effective than the individual ingredients. The functions of Lycium barbarum polysaccharide (LBP) glycoconjugate and grape seed procyanidins (GSP) are widely known. Here, we investigated the synergistic immune‐enhancing activity of LBP and GSP. Atomic force microscopy results suggested that the mixture of LBP and GSP exhibited circular structure unlike LBP alone, and the addition of polyphenols may change the spatial conformation of the sugar chain. The changes in the structure were related to the synergistic effect of the two functional agents on immune recovery. In vitro, the proliferation rate of splenocytes was higher in LBP + GSP group (64.16%), rather than the sum of LBP group (13.01%) and GSP group (43.61%) individually used. This synergistical proliferation of splenocytes may be correlated to the increasing intracellular free calcium levels. Furthermore, the mixture significantly enhanced the immunity in vivo, as evident from the recovery of peripheral white blood cell counts in LBP + GSP group (18.535 × 109/L) to normal group levels (18.115 × 109/L) and higher B cell proliferation than normal group (P < 0.05). These results highlight the immune‐enhancing activity of the combination of LBP and GSP associated with the structural changes, which may facilitate the development of functional foods with fewer resources but enhanced activities. Practical Application The synergistic effects of LBP and GSP on immunomodulatory were better than the sum of the effects of the individual agents both in vitro and in vivo. Our results may provide a research‐based support for the development of related functional products and an insight into the production of food resources with a fewer but more effective functional agents for better results.

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Downregulated Lipid Metabolism in Differentiated Murine Adipocytes by Procyanidins from Defatted Grape Seed Meal

Cited by 9 publications

References 38 publications

System-Level and Granger Network Analysis of Integrated Proteomic and Metabolomic Dynamics Identifies Key Points of Grape Berry Development at the Interface of Primary and Secondary Metabolism

System-Level and Granger Network Analysis of Integrated Proteomic and Metabolomic Dynamics Identifies Key Points of Grape Berry Development at the Interface of Primary and Secondary Metabolism

Obesity: The Metabolic Disease, Advances on Drug Discovery and Natural Product Research

A Mixture of Functional Complex Extracts from Lycium barbarum and Grape Seed Enhances Immunity Synergistically In Vitro and In Vivo

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