Myoblast proliferation following myotrauma is regulated by multiple factors including growth factors, signal pathways, transcription factors, and miRNAs. However, the molecular mechanisms underlying the orchestration of these regulatory factors remain unclear. Here we show that p38 signaling is required for miR-1/133a clusters transcription and both p38 activity and miR-1/133 expression are attenuated during the early stage of muscle regeneration in various animal models. Additionally, we show that both miR-1 and miR-133 reduce Cyclin D1 expression and repress myoblast proliferation by inducing G1 phase arrest. Furthermore, we demonstrate that miR-133 inhibits mitotic progression by targeting Sp1, which mediates Cyclin D1 transcription, while miR-1 suppresses G1/S phase transition by targeting Cyclin D1. Finally, we reveal that proproliferative FGF2, which is elevated during muscle regeneration, attenuates p38 signaling and miR-1/133 expression. Taken together, our results suggest that downregulation of p38-mediated miR-1/133 expression by FGF2 and subsequent upregulation of Sp1/Cyclin D1 contribute to the increased myoblast proliferation during the early stage of muscle regeneration.

In this study, we aimed to provide a large-scale set of psycholinguistic norms for 3,314 traditional Chinese characters, along with their naming reaction times (RTs), collected from 140 Chinese speakers. The lexical and semantic variables in the database include frequency, regularity, familiarity, consistency, number of strokes, homophone density, semantic ambiguity rating, phonetic combinability, semantic combinability, and the number of disyllabic compound words formed by a character. Multiple regression analyses were conducted to examine the predictive powers of these variables for the naming RTs. The results demonstrated that these variables could account for a significant portion of variance (55.8 %) in the naming RTs. An additional multiple regression analysis was conducted to demonstrate the effects of consistency and character frequency. Overall, the regression results were consistent with the findings of previous studies on Chinese character naming. This database should be useful for research into Chinese language processing, Chinese education, or crosslinguistic comparisons. The database can be accessed via an online inquiry system (http://ball.ling.sinica.edu.tw/ namingdatabase/index.html).Keywords Naming . Chinese character naming . Psycholinguistic norm . Chinese visual word recognition Understanding how readers process words, which are fundamental units for reading, has been a core issue in psycholinguistic studies. Behavioral studies have utilized tasks such as naming, lexical decision, speech perception, and synonym judgment, with the aim of understanding how orthographic, phonological, and semantic information is processed during visual word recognition. These experiments have typically employed a factorial design by manipulating one or two lexical variables (e.g., word frequency and spelling-to-sound consistency) in a limited set of stimuli, while trying to control for other lexical variables (e.g., orthographic neighborhood size and acoustic properties of onsets) in order to prevent any potential confounds. Although this approach has provided fruitful evidence to advance our understanding of how various lexical properties affect visual word recognition, it has some limitations in terms of how to select a set of items that vary in only one dimension and how to generalize the findings to a larger set of stimuli.To overcome these limitations, a number of large-scale psycholinguistic databases have been made available for languages with alphabetic orthographies, including English (Balota et al

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