Down-regulation of DMBT1 gene expression in human oral squamous cell carcinoma

Imai, Massao A.; Moriya, Tsuneo; Imai, Fabiana Lica; Shiiba, Masashi; Bukawa, Hiroki; Yokoe, Hidetaka; Uzawa, Katsuhiro; Tanzawa, Hideki

doi:10.3892/ijmm.15.4.585

Cited by 8 publications

(11 citation statements)

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“…Consistent with our finding, both the mRNA and protein levels of DMBT1 were determined to be lower in CSCC tissues than that in the adjacent normal tissues after examination of qRT‐PCR and Western blot methods, demonstrating a tumor suppressor role in CSCC. Notably, the downregulation of DMBT1 in many tumor cell lines and primary tumors has been reported to be possibly associated with allele loss, homozygous deletion, base substitution, structural rearrangement, or promoter methylation according to the previous studies, but its detailed mechanism of DMBT1 downexpression in CSCC has not been clarified, which would explore in our future research.…”

Section: Discussionmentioning

confidence: 88%

“…The stratified analysis demonstrated that DMBT1 positive expression had no relation with age (all P > .05), but was correlated to the FIGO stage, tumor diameter, lymph node metastasis, and differentiation of CSCC patients (all P < .05, Table 2). Besides, the effect of DMBT1 expression on the survival of CSCC patients was also analyzed been reported to be possibly associated with allele loss, homozygous deletion, base substitution, structural rearrangement, or promoter methylation according to the previous studies, [21][22][23] but its detailed mechanism of DMBT1 downexpression in CSCC has not been clarified, which would explore in our future research.…”

Section: Association Of Dmbt1 With Clinicopathological Features Andmentioning

confidence: 98%

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The protective role of DMBT1 in cervical squamous cell carcinoma

Zhang

2019

The Kaohsiung J of Med Scie

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To explore the possible influence of deleted in malignant brain tumor 1 (DMBT1) in cervical squamous cell carcinoma (CSCC). DMBT1 expression was detected by Real‐time reverse transcription PCR (qRT‐PCR) and immunohistochemistry in CSCC and adjacent normal tissues from 167 CSCC patients, and its relationship with clinicopathological features and prognosis was analyzed. Besides, the in vitro experiments, including MTT, Cell‐Light EdU, Wound‐healing, Transwell invasion, Annexin V‐FITC/PI staining, qRT‐PCR, and Western blot, were performed in SiHa and CaSKi cells, which were both divided into Blank, Vector, and DMBT1 groups. The mRNA level and the positive expression rate of DMBT1 in CSCC tissues were lower than the adjacent normal tissues. Moreover, DMBT1 positive rate was linked to FIGO stage, tumor diameter, lymph node metastasis, and tumor differentiation of CSCC. Besides, patients with positive DMBT1 expression had higher 5‐year survival rate than those negative ones. According to the in vitro experiments, SiHa and CaSKi cells with overexpressed DMBT1 showed the inhibition of proliferative ability and the enhancement of apoptosis with the upregulated pro‐apoptosis proteins (Bax and Cleaved caspase‐3) and down‐regulated anti‐apoptosis protein Bcl‐2. Moreover, compared with Blank group, DMBT1 group presented decrease in the migration and invasion of SiHa and CaSKi cells with the down‐expression of interstitial markers (N‐cadherin and Vimentin) and the up‐expression of epithelial marker E‐cadherin. DMBT1 was decreased in CSCC, whereas its overexpression can not only inhibit the proliferation, migration, and invasion, but induce the apoptosis of human CSCC cells, being a novel strategy for CSCC treatment.

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Section: Discussionmentioning

confidence: 88%

Section: Association Of Dmbt1 With Clinicopathological Features Andmentioning

confidence: 98%

The protective role of DMBT1 in cervical squamous cell carcinoma

Zhang

2019

The Kaohsiung J of Med Scie

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“…There is a large body of literature supporting an association of DMBT1 with cancer (2,5,23,26,35,37). However, other studies have failed to provide a link between DMBT1 and cancer (20,40,41).…”

Section: Discussionmentioning

confidence: 97%

Effects of Muclin (Dmbt1) deficiency on the gastrointestinal system

Lisle

Xu²,

Roe³

et al. 2008

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…Indeed, deregulation of DMBT1 expression has been reported for a number of tumors, including gliomas (Lin et al, 1998), small-and non-small-cell lung cancer cell lines and tumors (Takeshita et al, 1999;Wu et al, 1999;Mollenhauer et al, 2002), carcinoma of the esophagus (Mori et al, 1999;Mollenhauer et al, 2001), epithelial skin cancer , intrahepatic cholangiocarcinoma (Sasaki et al, 2003), breast cancer (Braidotti et al, 2004;Mollenhauer et al, 2004;Blackburn et al, 2007), salivary gland tumors (Bikker et al, 2004b), pancreatic ductal adenocarcinomas (Hustinx et al, 2004;Cheung et al, 2008), oral squamous cell carcinoma (Imai et al, 2005), gastric cancer (Conde et al, 2007), and cutaneous melanoma . In many cases, expression of DMBT1/gp340/SAG was deregulated not only at the tumor site but also in flanking tissue, which could arise as a consequence of the proinflammatory environment generated by the tumor.…”

Section: A Role In Epithelial Cell Differentiation and Pathogen Recomentioning

confidence: 99%