Down-regulation of adipogenesis of mesenchymal stem cells by oscillating high-gradient magnetic fields and mechanical vibration

Zablotskii, V.; Lunov, Oleg; Novotná, Božena; Churpita, O.; Trošan, Peter; Holáň, Vladimı́r; Syková, Eva; Dejneka, A.; Kubinová, Šárka

doi:10.1063/1.4895459

Cited by 34 publications

(29 citation statements)

References 23 publications

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“…The magnetic forces that are exerted on the cell body are transmitted to the cell cytoskeleton and cell membrane. Even tiny mechanical forces that are slightly larger than the thermal fluctuation forces of less 1 pN (see Methods) can significantly affect cell functionality32495051.…”

Section: Resultsmentioning

confidence: 99%

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How a High-Gradient Magnetic Field Could Affect Cell Life

Zablotskii

Polyakova

Lunov

et al. 2016

Sci Rep

166

123

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The biological effects of high-gradient magnetic fields (HGMFs) have steadily gained the increased attention of researchers from different disciplines, such as cell biology, cell therapy, targeted stem cell delivery and nanomedicine. We present a theoretical framework towards a fundamental understanding of the effects of HGMFs on intracellular processes, highlighting new directions for the study of living cell machinery: changing the probability of ion-channel on/off switching events by membrane magneto-mechanical stress, suppression of cell growth by magnetic pressure, magnetically induced cell division and cell reprograming, and forced migration of membrane receptor proteins. By deriving a generalized form for the Nernst equation, we find that a relatively small magnetic field (approximately 1 T) with a large gradient (up to 1 GT/m) can significantly change the membrane potential of the cell and thus have a significant impact on not only the properties and biological functionality of cells but also cell fate.

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Section: Resultsmentioning

confidence: 99%

“…Recent studies indicate the crucial influence of external mechanical and magnetic forces on the cell shape, function and fate through physical interactions with the cytoskeleton network464956.…”

Section: Resultsmentioning

confidence: 99%

How a High-Gradient Magnetic Field Could Affect Cell Life

Zablotskii

Polyakova

Lunov

et al. 2016

Sci Rep

166

123

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show abstract

“…External loads can also be applied by acoustic waves, mechanical vibrations and microbubble cavitation, among others (Box S1). 72–74 …”

Section: Manipulating Mechanobiologymentioning

confidence: 99%

Mechanical forces direct stem cell behaviour in development and regeneration

Vining

Mooney

2017

Nat Rev Mol Cell Biol

1,177

958

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Stem cells and their local microenvironment, or niche, communicate through mechanical, cues to regulate cell fate and cell behaviour, and to guide developmental processes. During embryonic development, mechanical forces are involved in patterning and organogenesis. The physical environment of pluripotent stem cells regulates their differentiation and self-renewal. Mechanical and physical cues are also important in adult tissues, where adult stem cells require physical interactions with the extracellular matrix to maintain their potency. In vitro, synthetic models of the stem cell niche can be used to precisely control and manipulate the biophysical and biochemical properties of the stem cell microenvironment and examine how the mode and magnitude of mechanical cues, such as matrix stiffness or applied forces, direct stem cell differentiation and function. Fundamental insights on the mechanobiology of stem cells also inform the design of artificial niches to support stem cells for regenerative therapies.

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“…The first study applied a low-frequency (1-10 Hz), high-gradient magnetic field and mechanical vibration to mesenchymal stem cells and showed that both mechanisms play an important role in F-actin remodeling and regulation of adipogenic differentiation. 37 The next two studies 38,39 incubated magnetic particles (iron oxide nanoparticles and permalloy microdisks, respectively) with cells in culture and applied AMFs. In the first one, 38 a hyperthermia-like field was employed (16 mT, 260 kHz), while in the second one, 39 a low-frequency field was used (9 mT, 20 Hz).…”

mentioning

confidence: 99%

Non-chemotoxic induction of cancer cell death using magnetic nanowires

Contreras¹,

Sougrat²,

Zaher³

et al. 2015

IJN

101

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In this paper, we show that magnetic nanowires with weak magnetic fields and low frequencies can induce cell death via a mechanism that does not involve heat production. We incubated colon cancer cells with two concentrations (2.4 and 12 μg/mL) of nickel nanowires that were 35 nm in diameter and exposed the cells and nanowires to an alternating magnetic field (0.5 mT and 1 Hz or 1 kHz) for 10 or 30 minutes. This low-power field exerted a force on the magnetic nanowires, causing a mechanical disturbance to the cells. Transmission electron microscopy images showed that the nanostructures were internalized into the cells within 1 hour of incubation. Cell viability studies showed that the magnetic field and the nanowires separately had minor deleterious effects on the cells; however, when combined, the magnetic field and nanowires caused the cell viability values to drop by up to 39%, depending on the strength of the magnetic field and the concentration of the nanowires. Cell membrane leakage experiments indicated membrane leakage of 20%, suggesting that cell death mechanisms induced by the nanowires and magnetic field involve some cell membrane rupture. Results suggest that magnetic nanowires can kill cancer cells. The proposed process requires simple and low-cost equipment with exposure to only very weak magnetic fields for short time periods.

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Down-regulation of adipogenesis of mesenchymal stem cells by oscillating high-gradient magnetic fields and mechanical vibration

Cited by 34 publications

References 23 publications

How a High-Gradient Magnetic Field Could Affect Cell Life

How a High-Gradient Magnetic Field Could Affect Cell Life

Mechanical forces direct stem cell behaviour in development and regeneration

Non-chemotoxic induction of cancer cell death using magnetic nanowires

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