How a High-Gradient Magnetic Field Could Affect Cell Life

Zablotskii, V.; Polyakova, T.; Lunov, Oleg; Dejneka, A.

doi:10.1038/srep37407

Cited by 167 publications

(147 citation statements)

References 73 publications

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“…speed, motion state, bend count, show differences between untreated worms and worms treated with particles, moving inside magnetic field, while they show no difference between untreated and particles-treated worms. Results are supported by recent work on the effect of magnetic field gradients on cells (10,68), mediated by magnetic nanoparticles (69). Our findings are added to the recent discoveries on C .…”

Section: Discussionsupporting

confidence: 70%

Caenorhabditis elegans locomotion is affected by internalized paramagnetic nanoparticles in the presence of magnetic field

Gourgou

Zhang

Mirzakhalili

et al. 2018

Preprint

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C . elegans with internalized magnetic nanoparticles are placed inside magnetic field to explore effects on locomotion. We hypothesize that internal magnetic fields created by nanoparticles induce localized effects on C . elegans ' locomotion machinery. To test our hypothesis, we use young adult hermaphrodite C . elegans fed on E . coli OP50 mixed with magnetic/paramagnetic nanoparticles of 1μm, 100nm and 40nm diameter. The presence of particles inside the worms' body is verified by fluorescent and electron microscopy. A custom-made software is used to track freely moving C . elegans in the absence or presence of magnetic field sequentially for 200+200sec. We use established metrics to quantify locomotion-related parameters, including posture, motion and path features. Results show that key features of C . elegans locomotion (e.g., speed, number of bends, motion state and range) are affected by the magnetic field in worms with internalized particles, in contrast to untreated worms, which remain unaffected. Our work contributes on clarifying the magnetic field effect on C . elegans locomotion and introduces C . elegans as a potential model system to explore in vivo the effect of magnetic field gradient on living organisms.

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Section: Discussionsupporting

confidence: 70%

Caenorhabditis elegans locomotion is affected by internalized paramagnetic nanoparticles in the presence of magnetic field

Gourgou

Zhang

Mirzakhalili

et al. 2018

Preprint

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“…[10] Thus, the presence of an HGMF alters the ion flux balance across the cell membrane, thereby changing the membrane potential. [10] Thus, the presence of an HGMF alters the ion flux balance across the cell membrane, thereby changing the membrane potential.…”

Section: Membrane Potentialmentioning

confidence: 99%

“…Tumor cells arrest (prediction) Cancer cells enriched in Fe 10 4 -10 5 T m À1 Unknown [10] 6 Cell swelling, ROS and apoptosis Human THP-1 leukemia cells 10 4 T m À1 24 h [8] 7…”

Section: Changes In Gene Expressionmentioning

confidence: 99%

“…Unknown effects related to the forced diffusion of paramagnetic molecules and plasma proteins Unknown 10 5 -10 6 T m À1 from milliseconds to seconds [55] 10 Change of membrane potential Any cells 10 9 T m À1 Instant [10] 11…”

Section: Changes In Gene Expressionmentioning

confidence: 99%

“…[10] Cells can miraculously sense magnetic forces [5,[11][12][13][14][15][16] and scientists rarely stop to consider how magnetic fields affect cellular functions and can be used to drive cell fate. [10] Cells can miraculously sense magnetic forces [5,[11][12][13][14][15][16] and scientists rarely stop to consider how magnetic fields affect cellular functions and can be used to drive cell fate.…”

Section: Introductionmentioning

confidence: 99%

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Cells in the Non‐Uniform Magnetic World: How Cells Respond to High‐Gradient Magnetic Fields

2018

Self Cite

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Imagine cells that live in a high-gradient magnetic field (HGMF). Through what mechanisms do the cells sense a non-uniform magnetic field and how such a field changes the cell fate? We show that magnetic forces generated by HGMFs can be comparable to intracellular forces and therefore may be capable of altering the functionality of an individual cell and tissues in unprecedented ways. We identify the cellular effectors of such fields and propose novel routes in cell biology predicting new biological effects such as magnetic control of cell-to-cell communication and vesicle transport, magnetic control of intracellular ROS levels, magnetically induced differentiation of stem cells, magnetically assisted cell division, or prevention of cells from dividing. On the basis of experimental facts and theoretical modeling we reveal timescales of cellular responses to high-gradient magnetic fields and suggest an explicit dependence of the cell response time on the magnitude of the magnetic field gradient.

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Levitational 3D Bioassembly and Density‐Based Spatial Coding of Levitoids

Moncal

Yaman

Durmus

2022

Adv Funct Materials

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Self‐assembly of cells into functional bioarchitectures with microscale spatial topographies are prevalent in nature. Despite the advances to recapitulate the native‐like microenvironment in vitro,fabrication of soft living materials with a deterministic control on the composition, functionality, and geometry, is still challenging. Here, a versatile, paramagnetically tunable, levitational biofabrication technique within a ring‐magnet system, RM‐LEV, is developed to assemble single cells or heterogeneous multicellular living architectures in a scaffold‐free manner. The self‐assembly and contactless biofabrication of multiple cell types into 3D multicellular “levitospheres” is demonstrated, where cellular positions are guided by levitation and density, simultaneously. Inherent density and diamagnetism of different cell types are leveraged to manipulate the geometry and self‐assembly of levitospheres into larger complex 3D structures, termed as “levitoids”. This approach is further applied to manipulate, position, and culture levitoids within a 3D hydrogel matrix under levitation, which preserves their viability and functionality. Thus, this study provides a foundation to create spatially heterogeneous 3D cellular assemblies with density‐coded bio‐architectures—which is not previously realized using magnetic levitation. This density‐based coding approach can be beneficial to study the cross‐talk between different cell types within spheroids and organoids, and be broadly applied to 3D bioprinting, tissue engineering, cancer, and neuroscience research.

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How a High-Gradient Magnetic Field Could Affect Cell Life

Cited by 167 publications

References 73 publications

Caenorhabditis elegans locomotion is affected by internalized paramagnetic nanoparticles in the presence of magnetic field

Caenorhabditis elegans locomotion is affected by internalized paramagnetic nanoparticles in the presence of magnetic field

Cells in the Non‐Uniform Magnetic World: How Cells Respond to High‐Gradient Magnetic Fields

Levitational 3D Bioassembly and Density‐Based Spatial Coding of Levitoids

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