Doubled Haploid Technology for Rapid and Efficient Maize Breeding

Chaikam, Vijay; Prasanna, B. M.

doi:10.1007/978-3-030-41866-3_11

Cited by 11 publications

(10 citation statements)

References 94 publications

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“…A haploid inducer hybrid, 2GTAIL006 × 2GTAIL009 [7], was used in all the haploid induction crosses, where both inducer parental lines were equipped with the R1-nj marker. Haploid induction nurseries were planted, managed, and crosses were carried out as described in a previous study [39].…”

Section: Haploid Induction Crossesmentioning

confidence: 99%

Effectiveness of R1-nj Anthocyanin Marker in the Identification of In Vivo Induced Maize Haploid Embryos

López

Ochieng

Pacheco

et al. 2023

Plants

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Doubled haploid (DH) technology has become integral to maize breeding programs to expedite inbred line development and increase the efficiency of breeding operations. Unlike many other plant species that use in vitro methods, DH production in maize uses a relatively simple and efficient in vivo haploid induction method. However, it takes two complete crop cycles for DH line generation, one for haploid induction and the other one for chromosome doubling and seed production. Rescuing in vivo induced haploid embryos has the potential to reduce the time for DH line development and improve the efficiency of DH line production. However, the identification of a few haploid embryos (~10%) resulting from an induction cross from the rest of the diploid embryos is a challenge. In this study, we demonstrated that an anthocyanin marker, namely R1-nj, which is integrated into most haploid inducers, can aid in distinguishing haploid and diploid embryos. Further, we tested conditions that enhance R1-nj anthocyanin marker expression in embryos and found that light and sucrose enhance anthocyanin expression, while phosphorous deprivation in the media had no affect. Validating the use of the R1-nj marker for haploid and diploid embryo identification using a gold standard classification based on visual differences among haploids and diploids for characteristics such as seedling vigor, erectness of leaves, tassel fertility, etc., indicated that the R1-nj marker could lead to significantly high false positives, necessitating the use of additional markers for increased accuracy and reliability of haploid embryo identification.

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Section: Haploid Induction Crossesmentioning

confidence: 99%

Effectiveness of R1-nj Anthocyanin Marker in the Identification of In Vivo Induced Maize Haploid Embryos

López

Ochieng

Pacheco

et al. 2023

Plants

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“…For instance, there is no scope for early generation selection, lack of recombination and its effectiveness varies across crosses. While considering the larger scale of breeding programs, doubled haploid breeding turns out to be expensive ( Chaudhary et al, 2019b ; Chaikam and Prasanna, 2020 ).…”

Section: Accelerated Plant Breeding Techniquesmentioning

confidence: 99%

Green revolution to genome revolution: driving better resilient crops against environmental instability

Chawla,

Poonia,

Samantara

et al. 2023

Front. Genet.

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Crop improvement programmes began with traditional breeding practices since the inception of agriculture. Farmers and plant breeders continue to use these strategies for crop improvement due to their broad application in modifying crop genetic compositions. Nonetheless, conventional breeding has significant downsides in regard to effort and time. Crop productivity seems to be hitting a plateau as a consequence of environmental issues and the scarcity of agricultural land. Therefore, continuous pursuit of advancement in crop improvement is essential. Recent technical innovations have resulted in a revolutionary shift in the pattern of breeding methods, leaning further towards molecular approaches. Among the promising approaches, marker-assisted selection, QTL mapping, omics-assisted breeding, genome-wide association studies and genome editing have lately gained prominence. Several governments have progressively relaxed their restrictions relating to genome editing. The present review highlights the evolutionary and revolutionary approaches that have been utilized for crop improvement in a bid to produce climate-resilient crops observing the consequence of climate change. Additionally, it will contribute to the comprehension of plant breeding succession so far. Investing in advanced sequencing technologies and bioinformatics will deepen our understanding of genetic variations and their functional implications, contributing to breakthroughs in crop improvement and biodiversity conservation.

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“…Since Generating doubled haploids, where the haploid embryo is rescued cultured and the chromosome is doubled through the use of colchicine or other mitotic inhibitors, such as nitrous oxide gas and some herbicides, the efficiency of selection for both qualitative and quantitative characters has increased due to complete homozygozity [15]. The haploid plant's chromosome counts are doubled in small sectors when a mitotic inhibitor is applied to the apical meristem.…”

Section: History Of Double Haploidsmentioning

confidence: 99%

Haploids and Doubled Haploid Technology Application in Modern Plant Breeding

Yali¹

2022

JPS

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Plant breeding, genetics, and genetic engineering all benefit from the use of doubled haploids technology. For genetic mapping of complicated phenotypes, the doubled haploid technique is a useful tool. To make doubled haploids, haploid cells (which are genetically unstable in the first place) can duplicate their genome at any point during their growth, resulting in diploid cells that don't require any additional therapies. The use of doubled haploidy in breeding is influenced by a variety of circumstances. Doubled haploids (DHs) are exploited in a variety of ways, depending on available technologies and species. In horticultural crops that are perennial in nature, outcrossing with inbreeding depression, or have high economic value where breeding time is critical, doubled haploids are gaining popularity as a powerful approach for enhancing genetic gain per cycle. For various reasons, the current methods for producing haploids and doubled haploids are primarily focused on the rapid generation of pure lines to speed hybrid seed production or CMS conversion, as well as the production of di-haploids to simplify breeding operations, such as in the case of potatoes. These approaches involve using methods based primarily on in vitro culture, in vivo induction of haploid development, or a combination of the two to make haploid embryos in vivo and then rescue them in vitro. Generally the aim of this review paper is to assess the technology of haploids, double haploids and their role in modern crop improvement program.

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Doubled Haploid Technology for Rapid and Efficient Maize Breeding

Cited by 11 publications

References 94 publications

Effectiveness of R1-nj Anthocyanin Marker in the Identification of In Vivo Induced Maize Haploid Embryos

Effectiveness of R1-nj Anthocyanin Marker in the Identification of In Vivo Induced Maize Haploid Embryos

Green revolution to genome revolution: driving better resilient crops against environmental instability

Haploids and Doubled Haploid Technology Application in Modern Plant Breeding

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