2017
DOI: 10.1038/s41598-017-07919-w
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Double-stranded RNA induces chicken T-cell lymphoma apoptosis by TRIF and NF-κB

Abstract: Toll-like receptor-3 (TLR3), a member of the pathogen recognition receptor family, has been reported to activate immune response and to exhibit pro-apoptotic activity against some tumor cells. However it is unclear whether TLR3 has same function against chicken lymphoma. In this paper we investigated the effect of TLR3 activation on a Marek’s disease lymphoma-derived chicken cell line, MDCC-MSB1. The TLR3 agonist poly (I:C) activated TLR3 pathway and inhibited tumor cells proliferation through caspase-dependen… Show more

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Cited by 6 publications
(5 citation statements)
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“…Two chicken immune cell lines, macrophage HD‐11 cells and B‐lymphocyte DT40 cells, were selected as 2 avian in vitro models for the innate and adaptive immune systems, respectively. Because both cell lines express toll‐like receptor 3 (TLR3), which is able to recognize double‐stranded RNA (dsRNA) related to viral infection (Peroval et al 2013; Zou et al 2017), polyinosinic–polycytidylic acid sodium salt (poly I:C), a synthetic analog of dsRNA (Chen et al 2013) was used as a viral activator via TLR3.…”
Section: Methodsmentioning
confidence: 99%
“…Two chicken immune cell lines, macrophage HD‐11 cells and B‐lymphocyte DT40 cells, were selected as 2 avian in vitro models for the innate and adaptive immune systems, respectively. Because both cell lines express toll‐like receptor 3 (TLR3), which is able to recognize double‐stranded RNA (dsRNA) related to viral infection (Peroval et al 2013; Zou et al 2017), polyinosinic–polycytidylic acid sodium salt (poly I:C), a synthetic analog of dsRNA (Chen et al 2013) was used as a viral activator via TLR3.…”
Section: Methodsmentioning
confidence: 99%
“…Based on the type II CRISPR-Cas system consisting of a single guide RNA (sgRNA), a trans-activating crRNA (tracrRNA) and a RNA-guided endonuclease Cas9 derived from Streptococcus pyogenes, the latest developed CRISPR/Cas9 gene editing technique has been displayed as a simple, efficient and powerful tool for gene editing in biological research [33][34][35]. It has not only been widely applied for generating gene knockout (KO) cell lines and animal models, but has also been used for manipulating large viral DNA genomes, such as Epstein-Barr virus (EBV) [36], herpes simplex virus type I (HSV-1) [32,37,38], guinea pig cytomegalovirus (GPCMV) [39], pseudorabies virus (PrV) [40][41][42], vaccinia virus (VACV) [43,44], and duck enteritis virus (DEV) [45,46]. We have successfully demonstrated the manipulation of the HVT and MDV-1 genomes using the CRISPR/Cas9 system both for generating recombinant vaccines and for studying the viral protein-coding gene functions respectively [47][48][49][50][51].…”
Section: Introductionmentioning
confidence: 99%
“…TLR3 stimulation with dsRNA is considered to directly promote tumor-cell apoptosis in many types of cancer, such as breast, melanoma, prostate, cervical, colon, and hepatocellular carcinoma [ 23 25 ]. Poly (I:C), a TLR3 activator, directly causes apoptosis in cancer cells via a caspase-dependent pathway [ 26 , 27 ].…”
Section: Discussionmentioning
confidence: 99%