Double Autoimmunostaining with Glycine Treatment

Hasui, Kazuhisa; Takatsuka, Tomio; Sakamoto, Ryoichi; Matsushita, Sachie; Tsuyama, Shinichiro; Izumo, Shuji; Murata, Fusayoshi

doi:10.1177/002215540305100907

Cited by 22 publications

(14 citation statements)

References 16 publications

(24 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…11 After deparaffinization and the inactivation of endogenous peroxidase, the antigen retrieval and immunostaining of anti-cleaved caspase-3 antibody (Asp175) was performed. The immunoreaction products except for DAB deposition were removed by means of glycine treatment.…”

Section: Double Immunostainingmentioning

confidence: 99%

“…The immunoreaction products except for DAB deposition were removed by means of glycine treatment. 11 Next, the antigen retrieval and immunostaining with either anti-Ki67 antigen antibody (MIB-1) or anti-p53 protein antibody (DO7) was performed. VIP (Vector VIP substrate kit SK-4600, Vector Laboratories, Burlingame, CA) was employed as a chromogen.…”

Section: Double Immunostainingmentioning

confidence: 99%

See 1 more Smart Citation

Association of High Proliferation in Adult T-cell Leukemia Cells With Apoptosis, and Expression of p53 Protein in Acute Type ATL

Wang

Hasui

Utsunomiya

et al. 2008

J Clin Exp Hematopathol

Self Cite

View full text Add to dashboard Cite

Proliferation, apoptosis and p53 protein expression in adult T-cell leukemia (ATL) cells were investigated. Twenty peripheral blood tissue specimens (PBTS) comprising 7 cases of acute type ATL, 7 cases of chronic type ATL and 6 other leukemias were examined by means of antigen retrieval and the polymer method employing anti-Ki67 antigen (MIB-1), anti-cleaved caspase-3, anti-single stranded DNA and three kinds of anti-p53 protein antibodies including DO7. Most acute and chronic cases of ATL included more than 10% MIB-1-positive proliferating leukemia cells and more than 1% cleaved caspase-3-positive apoptotic cells. Some cells which were positive for both MIB-1 and anti-cleaved caspase-3 antibody were observed in acute type ATL. Nuclear deposition of p53 protein labeled by DO7 was often found in acute type (p < 0.05). Within the medium-sized population of ATL cell nuclei, DO7-positive ATL cells had a smaller nuclear area factor (long axis x short axis) than DO7-negative ATL cells. A few proliferating ATL cells entered apoptosis, and the appearance of a subclone of ATL cells with nuclear deposition of p53 protein labeled by DO7 characterized acute type.

show abstract

Section: Double Immunostainingmentioning

confidence: 99%

Section: Double Immunostainingmentioning

confidence: 99%

Association of High Proliferation in Adult T-cell Leukemia Cells With Apoptosis, and Expression of p53 Protein in Acute Type ATL

Wang

Hasui

Utsunomiya

et al. 2008

J Clin Exp Hematopathol

Self Cite

View full text Add to dashboard Cite

show abstract

“…For AR and pituitary hormone dual staining, all the primary antibodies are raised from the rabbit because successive or simultaneous localization of two antigens by immunohistochemistry using two same source antibodies has been reported (Hasui et al 2003). AR staining was Wrstly performed as described above, except that the tertiary antibody was Xuorescein isothiocyanate (FITC)-conjugated streptavidin (SouthernBiotech, Birmingham, AL, USA; 1:60).…”

Section: Immunohistochemistrymentioning

confidence: 99%

Expression of androgen receptor and its co-localization with estrogen receptor-alpha in the developing pituitary gland of sheep fetus

Yuan

Liu

et al. 2007

Histochem Cell Biol

View full text Add to dashboard Cite

No information is known concerning the expression of androgen receptor (AR) and its co-localization with estrogen receptor alpha (ERalpha) in the developing pituitary of sheep fetus. In the present study, we detected AR expression and its co-localization with ERalpha in the anterior pituitary of sheep fetus from day 60 of gestation to the postnatal by dual immunochemistry. The results showed that both AR immunoreactivity (AR-ir) and ERalpha immunoreactivity (ERalpha-ir) were predominantly localized in the nuclei of LH positive gonadotropes. The cell counting results showed that the percentage of the anterior pituitary cells expressing AR fluctuated from 13.51 +/- 0.92 to 17.05 +/- 1.83% during the examined stages, but there were no significant differences between sexes and among ages examined (P > 0.05). However, the proportion of AR-ir cells containing LH markedly increased from day 60 of gestation to the neonatal (P < 0.05). The percentage of AR-ir cells expressing ERalpha-ir significantly increased from day 60 of gestation to the neonatal, respectively (P < 0.05), but no significant differences were seen between genders at each stage examined. These results indicate that both AR and ERalpha are mainly expressed in the gonadotropes of anterior pituitary gland of sheep fetuses, whereas the functions and interaction of AR and ERalpha expressions in the developing pituitary gland are required to be elucidated further.

show abstract

“…10 First, the sections were immunolabeled with the anti-CD68 or anti-MHC class II antibody and then visualized with DAB (brown in color) as mentioned above. Second, sections used for CD163 were reacted with anti-CD68 or anti-MHC class II antibody, as well as sections for MHC class II with anti-CD68 antibody.…”

Section: Double Immunohistochemical Labelingmentioning

confidence: 99%

Immunophenotypical Characterization of Macrophages in Rat Bleomycin-Induced Scleroderma

et al. 2012

View full text Add to dashboard Cite

Scleroderma is a skin disorder characterized by persistent fibrosis. Macrophage properties influencing cutaneous fibrogenesis remain to be fully elucidated. In this rat (F344 rats) model of scleroderma, at 1, 2, 3, and 4 weeks after initiation of daily subcutaneous injections of bleomycin (BLM; 100 ml of 1 mg/ml daily), skin samples were collected for histological and immunohistochemical evaluations. Immunohistochemically, the numbers of cells reacting to ED1 (anti-CD68; phagocytic activity) and ED2 (anti-CD163; inflammatory factor production) began to increase at week 1, peaked at week 2, and decreased thereafter. In contrast, the increased number of cells reacting to OX6 (anti-MHC class II molecules) was seen from week 2 and remained elevated until week 4. aSmooth muscle actin-positive myofibroblasts were increased for 4 weeks. Double labeling revealed that galectin-3, a regulator of fibrogenic factor TGF-b1, was expressed in CD68þ, CD163þ, and MHC class IIþ macrophages and myofibroblasts. mRNA expression of TGF-b1, as well as MCP-1 and CSF-1 (both macrophage function modulators), were significantly elevated at weeks 1 to 4. This study shows that the increased number of macrophages with heterogeneous immunophenotypes, which might be induced by MCP-1 and CSF-1, could participate in the sclerotic lesion formation, presumably through increased fibrogenic factors such as galectin-3 and TGF-b1; the data may provide useful information to understand the pathogenesis of the human scleroderma condition.

show abstract

Double Autoimmunostaining with Glycine Treatment

Cited by 22 publications

References 16 publications

Association of High Proliferation in Adult T-cell Leukemia Cells With Apoptosis, and Expression of p53 Protein in Acute Type ATL

Association of High Proliferation in Adult T-cell Leukemia Cells With Apoptosis, and Expression of p53 Protein in Acute Type ATL

Expression of androgen receptor and its co-localization with estrogen receptor-alpha in the developing pituitary gland of sheep fetus

Immunophenotypical Characterization of Macrophages in Rat Bleomycin-Induced Scleroderma

Contact Info

Product

Resources

About