Double-antibody sandwich ELISA using biotinylated antibodies for the detection of Echinococcus granulosus coproantigens in dogs

Benito, Aitziber; Carmena, David

doi:10.1016/j.actatropica.2005.03.005

Cited by 19 publications

(10 citation statements)

References 23 publications

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“…28 The sensitivity of our test was higher than previously reported copro-ELISAs that used polyclonal antibodies. 13,[24][25][26] Two false-negative samples were observed in the present study; however, one of these had only one parasite, whereas the other was sampled at 20 days postinfection with E. granulosus. The parasite burden and the postinfection time may have affected detection due to fewer parasitic antigens being produced by a lower number of worms 29 or due to the antigen presentation of immature worms in early infection.…”

Section: Discussioncontrasting

confidence: 47%

“…Few copro-ELISA tests based on the detection of somatic, excretory, or secretory antigens of E. granulosus have been developed with variable sensitivity and specificity. [24][25][26][27] All of these tests have a higher sensitivity than arecoline purgation and PCR for the detection of prepatent infection in dogs. 28 The sensitivity of our test was higher than previously reported copro-ELISAs that used polyclonal antibodies.…”

Section: Discussionmentioning

confidence: 99%

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Development and Validation of a Copro-Enzyme–Linked Immunosorbent Assay Sandwich for Detection of Echinococcus granulosus–Soluble Membrane Antigens in Dogs

Jara

Rodríguez

Altamirano

et al. 2019

The American Journal of Tropical Medicine and Hygiene

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Cystic echinococcosis (CE) is a parasitic zoonosis caused by the larval stage of the tapeworm Echinococcus granulosus. Detection of the adult stage in the canine definitive host is essential for estimating infection rates, surveillance and monitoring of CE control programs. This study sought to develop and validate a coproantigen sandwich enzyme-linked immunosorbent assay (copro-ELISA), based on antibodies against E. granulosus-soluble membrane antigens (EGMA), that is capable of distinguishing infected and noninfected dogs. Anti-E. granulosus polyclonal immunoglobulin G antibodies were obtained from rabbit antiserum against EGMA. Optimization of the test was performed with 51 positive and 56 negative stool samples of canine echinococcosis. Specificity, sensitivity, cross-reactivity, intra-and inter-assay precision, and over time detection were evaluated. According to the receiver operating characteristic analysis, the diagnostic sensitivity and specificity were 96.1% (CI: 85.9-99.6) and 98.2% (CI: 89.5-100), respectively. Negative and positive predictive values were 96.5% (CI: 91.7-100) and 98% (CI: 94.1-100), respectively. No cross-reactivity with Taenia hydatigena, Dipylidium caninum, or Toxocara canis was observed. Intra-and inter-assay repeatability showed values of less than 15% of the variation coefficient. The over time detection was from 20 to 27 days postinfection with E. granulosus. The copro-ELISA based on EGMA detection offers a simplified in-house development of diagnostic testing. This assay showed high specificity and sensitivity and had no cross-reactivity with other parasites. Further studies and development of this test in a kit format may be useful for the detection of active infection in dogs living in CE endemic regions.

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Section: Discussioncontrasting

confidence: 47%

Section: Discussionmentioning

confidence: 99%

Development and Validation of a Copro-Enzyme–Linked Immunosorbent Assay Sandwich for Detection of Echinococcus granulosus–Soluble Membrane Antigens in Dogs

Jara

Rodríguez

Altamirano

et al. 2019

The American Journal of Tropical Medicine and Hygiene

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“…Machnicka et al, 2003) or primarily for E. granulosus (Buishi et al, 2005a). For canine echinococcosis due to E. granulosus most authors report reasonable sensitivity (78.4 -100% (Allan et al, 1992;Benito and Carmena, 2005;Buishi et al, 2005a) and good genus specificity from 85 to greater than 95% (Allan et al, 1992;Benito and Carmena, 2005;Buishi et al, 2005a), as well as a degree of pre-patent detection (Deplazes et al, 1992;Jenkins et al, 2000). Where cross-reactions occur these generally appear to be caused by infection with Taenia hydatigena, the most common taeniid of dogs, and attempts to improve specificity by using monoclonal antibodies in coproELISAs have not been able to eliminate this problem (Malgor et al, 1997;Morel et al, 2013) (Table 1).…”

Section: Coproantigen Elisa For Detection Of Canine Echinococcosismentioning

confidence: 91%

“…To create polyclonal antibodies against Echinococcus spp., rabbits were hyperimmunised with Echinococcus antigens, such as adult or protoscolex ES extracts (e.g. Benito and Carmena, 2005), or somatic extracts of adult tapeworms (e.g. Allan et al, 1992).…”

Section: Coproantigen Elisa For Detection Of Canine Echinococcosismentioning

confidence: 99%

Echinococcus granulosus: Epidemiology and state-of-the-art of diagnostics in animals

Craig

Mastin

Kesteren

et al. 2015

Veterinary Parasitology

118

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Diagnosis and detection of Echinococcus granulosus (sensu lato) infection in animals is a prerequisite for epidemiological studies and surveillance of echinococcosis in endemic, re-emergent or emergent transmission zones. Advances in diagnostic approaches for definitive hosts and livestock, however, have not progressed equally over the last 20 years. Development of laboratory based diagnostics for canids using coproantigen ELISA and also coproPCR, have had a huge impact on epidemiological studies and more recently on surveillance during hydatid control programmes. In contrast, diagnosis of cystic echinococcosis (CE) in livestock still relies largely on conventional post-mortem inspection, despite a relatively low diagnostic sensitivity especially in early infections, as current serodiagnostics do not provide a sufficiently specific and sensitive practical pre-mortem alternative. As a result, testing of dog faecal samples by coproantigen ELISA, often combined with mass ultrasound screening programmes for human CE, has been the preferred approach for monitoring and surveillance in resource-poor endemic areas and during control schemes. In this article we review the current options and approaches for diagnosis of E. granulosus infection in definitive and animal intermediate hosts (including applications in non-domesticated species) and make conclusions and recommendations for further improvements in diagnosis for use in epidemiological studies and surveillance schemes.

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“…infection have been developed (Deplazes and Eckert, 1996;Benito and Carmena, 2005;Benito et al, 2006;Huang et al, 2007). The CoproAg-ELISA used was developed to detect adult infections of E. multilocularis (Sakashita et al, 1995) and E. granulosus (Malgor et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Ulnar mononeuropathy associated with eosinophilia

Kumar

Sodhi

2012

Paediatrics and International Child Health

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Double-antibody sandwich ELISA using biotinylated antibodies for the detection of Echinococcus granulosus coproantigens in dogs

Cited by 19 publications

References 23 publications

Development and Validation of a Copro-Enzyme–Linked Immunosorbent Assay Sandwich for Detection of Echinococcus granulosus–Soluble Membrane Antigens in Dogs

Development and Validation of a Copro-Enzyme–Linked Immunosorbent Assay Sandwich for Detection of Echinococcus granulosus–Soluble Membrane Antigens in Dogs

Echinococcus granulosus: Epidemiology and state-of-the-art of diagnostics in animals

Ulnar mononeuropathy associated with eosinophilia

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