Dot immunobinding assay for simultaneous detection of specific immunoglobulin G antibodies to measles virus, mumps virus, and rubella virus

Condorelli, Francesca; Ziegler, Thedi

doi:10.1128/jcm.31.3.717-719.1993

Cited by 12 publications

(2 citation statements)

References 14 publications

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“…Previous DBS‐based studies measuring measles immunity have evaluated methods for quantifying immunoglobulin M (IgM) [De Swart et al, ; Helfand et al, ; Riddell et al, ; Mubarak et al, ; Uzicanin et al, ], a biomarker of recent exposure to the virus, and immunoglobulin G (IgG) [Condorelli and Ziegler, ; Condorelli et al, ; De Swart et al, ; Helfand et al, ; Riddell et al, ; Ibrahim et al, ; Mercader et al, ; Samoĭlovich et al, ], a biomarker of past exposure to the virus or vaccine. IgG is most relevant for evaluating the effectiveness of immunization programs.…”

Section: Introductionmentioning

confidence: 99%

“…IgG is most relevant for evaluating the effectiveness of immunization programs. The methods employed in the IgG‐based studies included hemagglutination inhibition [Nakano et al, ; Wassilak et al, ; Rawat et al, ], dot immunobinding [Condorelli and Ziegler, ], RNA detection [De Swart et al, ; Katz et al, ; Riddell et al, ; Mubarak et al, ; Mosquera et al, ; Uzicanin et al, ], and enzyme immunoassays (EIA) [Condorelli et al, ; De Swart et al, ; Helfand et al, ; Ibrahim et al, ]. The EIA‐related enzyme‐linked immunosorbent assay (ELISA) is an attractive method given the commercial availability of a variety of kits and hence suitability for use in cross‐study and cross‐laboratory comparisons.…”

Section: Introductionmentioning

confidence: 99%

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Use of a commercial ELISA for the detection of measles-specific immunoglobulin G (IgG) in dried blood spots collected from children living in low-resource settings

et al. 2015

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Seroepidemiological monitoring of population immunity to vaccine-preventable diseases is critical to prevent future outbreaks. Dried blood spots (DBS), drops of capillary blood dried on filter paper, are an affordable, minimally invasive alternative to venipuncture for collecting blood in field settings. However, few proven methods exist to analyze DBS for the presence of protective antibodies. This study validates a novel technique for measuring measles-specific immunoglobulin G (IgG) in capillary DBS using a commercial ELISA. The predictive performance of a new method for analyzing DBS was tested by comparing matched serum and DBS samples from 50 children. The accuracy, precision, and reliability of the procedure were evaluated, and the optimal cut points to classify positive and negative samples were determined. The method was then applied to 1,588 DBS collected during a large survey of children in Mexico and Nicaragua. Measles-specific IgG in serum samples were 62% negative, 10% equivocal, and 28% positive. In comparisons with matched serum, DBS results were 100% sensitive and 96 · 8% specific, and agreed in 46 of 50 (92%) cases. The inter-assay and intra-assay coefficients of variation from kit-provided controls were greater than desired (24.8% and 8.4%, respectively). However, in predictive simulations the average misclassification was only 3.9%. Procedures were found to be acceptable to surveyors and participants. Analyzing DBS collected in low-resources settings is a feasible and accurate means of measuring population immunity to measles and should be used to generate objective measures of health status and health system performance.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Use of a commercial ELISA for the detection of measles-specific immunoglobulin G (IgG) in dried blood spots collected from children living in low-resource settings

et al. 2015

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Use of a microquantity enzyme immunoassay in a large-scale study of measles, mumps, and rubella immunity in Italy

Condorelli

Stivala

Gallo

et al. 1998

Eur. J. Clin. Microbiol. Infect. Dis.

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The seroprevalence of antibodies to measles, mumps, and rubella viruses (MMR) was determined in 1498 subjects in Catania, Italy, ranging in age from 1 month to 25 years. The study population was divided into seven age groups and screened by enzyme immunoassay using microquantities (10 microl) of whole blood collected by fingerprick on filter paper discs. The results showed that seroconversion for measles (87.6%) and mumps (73.2%) occurred between 6 and 10 years of age. The seroprevalence of antibodies to rubella virus increased slowly through the age groups, reaching the highest rate (93.3%) between 16 and 20 years of age. Passively transmitted maternal antibodies to mumps and rubella were absent in babies between 5 and 8 months of age, and a few cases positive for measles antibodies were found among babies 6 and 7 months of age. The enzyme immunoassay was demonstrated to be suitable for low-cost large-scale screening for MMR immunity. The rate of vaccine failure was also evaluated and found to be 9.5% for the measles virus, 12.9% for the mumps virus and 0.0% for the rubella virus.

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Detection of immunoglobulin G to measles virus, rubella virus, and mumps virus in serum samples and in microquantities of whole blood dried on filter paper

Condorelli

Scalia

Stivala

et al. 1994

Journal of Virological Methods

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Dot immunobinding assay for simultaneous detection of specific immunoglobulin G antibodies to measles virus, mumps virus, and rubella virus

Cited by 12 publications

References 14 publications

Use of a commercial ELISA for the detection of measles-specific immunoglobulin G (IgG) in dried blood spots collected from children living in low-resource settings

Use of a commercial ELISA for the detection of measles-specific immunoglobulin G (IgG) in dried blood spots collected from children living in low-resource settings

Use of a microquantity enzyme immunoassay in a large-scale study of measles, mumps, and rubella immunity in Italy

Detection of immunoglobulin G to measles virus, rubella virus, and mumps virus in serum samples and in microquantities of whole blood dried on filter paper

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