Detection of immunoglobulin G to measles virus, rubella virus, and mumps virus in serum samples and in microquantities of whole blood dried on filter paper

Condorelli, Francesca; Scalia, Guido; Stivala, Aldo; Gallo, Rita; Marino, Anna Maria Fausta; Battaglini, Chiara M.; Castro, A.

doi:10.1016/0166-0934(94)90052-3

Cited by 45 publications

(33 citation statements)

References 18 publications

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“…While the usefulness of filter paper blood samples for the measurement of MV-specific serum antibodies had been demonstrated before (3,12), recent publications have suggested that filter paper blood samples may also be suitable for RT-PCR analyses to diagnose certain virus infections (1,15). In the present study we show that blood samples spotted on filter paper are indeed suitable for use in MV-specific RT-PCR analysis.…”

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confidence: 49%

Combination of Reverse Transcriptase PCR Analysis and Immunoglobulin M Detection on Filter Paper Blood Samples Allows Diagnostic and Epidemiological Studies of Measles

et al. 2001

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As measles control and elimination campaigns progress, laboratory confirmation of clinically diagnosed measles cases becomes increasingly important. However, in many tropical countries collection and storage of clinical specimens for this purpose are logistically complicated. In this study it is shown that blood samples spotted on filter paper are suitable for the laboratory diagnosis of measles using a combination of reverse transcriptase PCR (RT-PCR) analysis and immunoglobulin M (IgM) detection. First, it was shown that in vitro measles virus (MV)-infected cells diluted in human blood and spotted on filter paper can be detected by RT-PCR. Small amounts of infected cells remained detectable after 25 weeks of storage of the filter paper at room temperature, 4 weeks at 37°C, or 2 weeks at 45°C. Subsequently, this RT-PCR was applied to filter paper blood samples collected from 117 clinically diagnosed measles patients in Sudan in 1997 and 1998. Prior laboratory diagnosis had confirmed 90 cases as acute MV infections, while 27 proved to be nonmeasles rash disease cases. Positive RT-PCR signals were detected in filter paper blood samples of 43 of the 90 confirmed cases (48%) but in none of the 27 nonmeasles cases. In addition, MV-specific IgM levels measured in reconstituted filter paper samples correlated well with those measured in plasma samples. Measles diagnosis based on the combination of filter paper RT-PCR and IgM detection had a sensitivity and specificity of 99 and 96%, respectively. An advantage of this diagnostic approach is that sequencing of RT-PCR products allows phylogenetic analysis of the MV strain involved.

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confidence: 49%

Combination of Reverse Transcriptase PCR Analysis and Immunoglobulin M Detection on Filter Paper Blood Samples Allows Diagnostic and Epidemiological Studies of Measles

et al. 2001

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“…It has previously been demonstrated that dried blood spots are a feasible alternative to venous blood as a specimen for use in investigations for measles virus antibodies (2,3,5,10). The present study demonstrates for the first time the feasibility of laboratory diagnosis of measles by use of dried blood specimens with a commercial assay.…”

Section: Discussionmentioning

confidence: 70%

“…Several groups have demonstrated a good correlation between serum and peripheral (finger or heel) blood for determination of measles virus antibody titers (2,3,10,15). Our results should therefore be equally applicable to capillary blood specimens.…”

Section: Discussionmentioning

confidence: 94%

“…The use of dried blood spots for the investigation of measles virus hemagglutination inhibition antibodies was reported in the early 1980s (9,15). Since then several other laboratories have demonstrated the feasibility of using dried blood samples in an enzyme immunoassay (EIA) format for the detection of measles virus-specific immunoglobulin G (IgG) (2,10) and IgM (3). In a small study, Novello et al (10) demonstrated the use of dried blood samples with the Dade Behring measles virus-specific IgG EIA for the investigation of immunity to measles virus.…”

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confidence: 99%

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Detection of Measles Virus-Specific Immunoglobulin M in Dried Venous Blood Samples by Using a Commercial Enzyme Immunoassay

et al. 2002

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The optical densities (ODs) of 216 dried venous blood (DVB) samples submitted to the Victorian Infectious Diseases Reference Laboratory as part of enhanced measles surveillance were compared to the ODs of the corresponding serum samples collected at the same time. DVB samples, stored for up to 24 months at 4°C, were tested by the Dade Behring Enzygnost Anti-Measles-Virus/IgM immunoassay. Elution and testing conditions were optimized with the use of spiked DVB samples. The assay showed an overall sensitivity of 90.2% and a specificity of 98.8% for DVB samples compared to the results for serum. When the results were analyzed according to the length of time that the DVB sample had been stored, the assay was 100% sensitive and 97% specific according to the ODs for those samples stored for less than 6 months compared to the results for the corresponding serum samples, with 97.7% agreement between the results for the two sample types. These results demonstrate the potential for the use of DVB samples for the diagnosis of measles in routine diagnostic laboratories.

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“…The utility of dried blood spots on filter paper has also been established for the detection of MV-specific antibodies (5,11,17) and the detection of MV RNA by RT-PCR (6,14). However, we have shown that blood for the detection of MV RNA by RT-PCR is a less optimal specimen type than samples collected from the respiratory tract, where MV RNA remains detectable for a period of up to 2 weeks after rash onset (21).…”

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confidence: 91%

Applicability of Oral Fluid Collected onto Filter Paper for Detection and Genetic Characterization of Measles Virus Strains

et al. 2005

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Expansion of measles molecular surveillance to developing countries where measles is endemic will help facilitate measles control. Limited infrastructure in these areas is a barrier to referral of specimens suitable for measles virus (MV) genotyping. In this study, we demonstrate that oral fluid dried onto filter paper can be used for the detection and characterization of MV strains. Using this approach, an MV-positive sample by reverse transcriptase PCR could be obtained from 67% of serologically confirmed acute measles cases. Mimicking certain environmental conditions and duration of transportation established that MV RNA remained detectable and suitable for nucleic acid sequencing in oral fluid spots for at least 1 week. In the context of a measles outbreak in a remote region of the world where infrastructure is poor, oral fluid samples dried onto filter paper and sent to a specialized laboratory for testing will aid in the identification and characterization of the causative MV strain.

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Detection of immunoglobulin G to measles virus, rubella virus, and mumps virus in serum samples and in microquantities of whole blood dried on filter paper

Cited by 45 publications

References 18 publications

Combination of Reverse Transcriptase PCR Analysis and Immunoglobulin M Detection on Filter Paper Blood Samples Allows Diagnostic and Epidemiological Studies of Measles

Combination of Reverse Transcriptase PCR Analysis and Immunoglobulin M Detection on Filter Paper Blood Samples Allows Diagnostic and Epidemiological Studies of Measles

Detection of Measles Virus-Specific Immunoglobulin M in Dried Venous Blood Samples by Using a Commercial Enzyme Immunoassay

Applicability of Oral Fluid Collected onto Filter Paper for Detection and Genetic Characterization of Measles Virus Strains

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