Dot immuno binding (DIB) for detection of virus in seed

Lange, Lene; Heide, M.

doi:10.1080/07060668609501772

Cited by 19 publications

(5 citation statements)

References 9 publications

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“…The positive reaction is indicated with coloured dots. The presence of barley stripe mosaic virus and bean common mosaic virus in a single seed of French bean could be detected by the DIBA technique (Lange and Heide 1986). DIBA is optimized and used successfully for the rapid detection of 15 known soybean viruses as well (Ali 2017).…”

Section: Immunoassay Methodsmentioning

confidence: 99%

Seed Health: Testing and Management

Vishunavat

Prabakar

Anand

2023

Seed Science and Technology

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Healthy seeds play an important role in growing a healthy crop. Seed health testing is performed by detecting the presence or absence of insect infestation and seed-borne diseases caused by fungi, bacteria, and viruses. The most detrimental effect of seed-borne pathogens is the contamination of previously disease-free areas and the spread of new diseases. Sowing contaminated or infected seeds not only spreads pathogens but can also reduce yields significantly by 15–90%. Some of the major seed-borne diseases affecting yield in cereals, oilseeds, legumes, and vegetables, particularly in the warm and humid conditions prevailing in the tropical and sub-tropical regions, are blast and brown spot of rice, white tip nematode and ear-cockle in wheat, bacterial leaf blight of rice, downy mildews, smuts, head mould, seedling rots, anthracnose, halo blight, and a number of viral diseases. Hence, detection of seed-borne pathogens, such as fungi (anthracnose, bunt, smut, galls, fungal blights), bacteria (bacterial blights, fruit rots, cankers), viruses (crinkle, mottle, mosaic), and nematodes (galls and white tip), which transmit through infected seed to the main crop, is an important step in the management strategies for seed-borne diseases. Thus, seed health testing forms an essential part of seed certification, phytosanitary certification, and quarantine programmes at national and international levels. Detection of seed-borne/transmitted pathogens is also vital in ensuring the health of the basic stock used for seed production and in maintaining the plant germplasm for future research and product development. Besides the precise and reproducible testing methods, appropriate practices during seed production and post-harvest handling, including seed treatment and storage, are important components of seed health management and sustainable crop protection.

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Section: Immunoassay Methodsmentioning

confidence: 99%

Seed Health: Testing and Management

Vishunavat

Prabakar

Anand

2023

Seed Science and Technology

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“…Development of a colored dot indicates the positive reaction. The presence of Barley stripe mosaic virus and Bean common mosaic virus in single seed of French bean could be detected by DIBA technique (Lange and Heide, 1986). DIBA was reported to be slightly more sensitive than ELISA for the detection of Pea seed-borne mosaic virus (Lange, 1986).…”

Section: Viral Pathogensmentioning

confidence: 99%

Detection and Identification of Postharvest Microbial Pathogens

Narayanasamy

2005

Postharvest Pathogens and Disease Management

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“…The addition of virus IgG produced in rabbit and the anti-rabbit IgG produced in mouse follow protocols similar to indirect ELISA or PTA-ELISA (Figure 3), except that the positive reactions in DIBA are recorded as colored dots on the membrane. Considering that DIBA is a simple, less laborious and quick test, it can be used routinely for plant virus indexing and survey programs (Almeida, 2001;Astier et al, 2007;Banttari & Goodwin, 1985;Graddon & Randles, 1986;Heide & Lange, 1988;Lange & Heide, 1986;Makkouk et al, 1993;Purcifull et al, 2001;Rybicki & Von Wechmar, 1982). One disadvantage of DIBA is the possibility of sap components interfering with the antigen-antibody reactions, resulting in subsequent problems with the diagnostic results.…”

Section: Dot Blot or Dot Immuno Binding Assay (Diba)mentioning

confidence: 99%