Dot blot ELISA on Nitrocellulose membranes (NC): a new method for typing Lewis and ABO of body fluids (saliva, semen, vaginal secretions, sweat and urine)

ABO blood grouping of saliva from mixed body fluids by sandwich methods using monoclonal antibodies to tissue specific epitopes on blood group substance in saliva

“…The detection limits of A B O blood group epitopes from non-secretor saliva are much lower than secretor saliva using this methods. This phenomenon was also seen in dot E L I S A [6]. The sensitivity of the sandwich absorption-elution test is lower than sandwich E L I S A for the detection of A B O -B G E s from secretor saliva.…”

“…As shown in Fig. 3, the detection limits of ABO-blood group epitopes using the sandwich E L I S A were somewhat lower than E L I S A or dot E L I S A methods which were used to detect A B O blood groups from saliva with no contamination from other body fluids [5,6]. The detection limits of A B O blood group epitopes from non-secretor saliva are much lower than secretor saliva using this methods.…”