ABO blood grouping of saliva from mixed body fluids by sandwich methods using monoclonal antibodies to tissue specific epitopes on blood group substance in saliva

Abstract

In this paper methods for ABO blood grouping of saliva from mixed body fluids have been established. Monoclonal antibodies to tissue specific epitopes on blood group substances in saliva were used as solid phase antibodies to catch the blood group substances. ABO blood grouping of saliva could be performed by these methods without interference from other body fluids (eg. semen, vaginal secretion, urine, sweat and serum). At least 16,000 and 3,000 fold dilutions of secretor saliva were sufficient for ABO blood grouping by sandwich ELISA and sandwich absorption-elution test, respectively.

Saliva in identification of ABO Blood Grouping Rhesus Factor

“…Saboor M et al, [13] conducted a study to evaluate the ABH blood group among 101 healthy adult students and concluded that the frequency was highest in blood group B which was in accordance with our study. It is also seen that out of 50 subjects, 48 of them showed the secretor level similar to the study conducted by Pereira M et al, [14] Kimura A et al, [15] Parekh P et al, [16] and Pawan Motghare et al [4] . This study also shows that the ability to secrete ABO blood group antigens in saliva was more common in males than in females.The correlation (Table 2) proves that saliva can be used in identification of ABO Blood grouping and Rh factor ( p < 0.001.…”

ABO blood grouping of semen from mixed body fluids with monoclonal antibody to tissue-specific epitopes on seminal ABO blood group substance

“…These mAbs recognized the core protein of salival BGS and were tissue and species specific [1], and could be employed for blood grouping of A. Kimura et al: ABO from mixed body fluids saliva from mixed body fluids [2]. We think that the sandwich method with mAb against TSE of BGS may be the universal method for ABO blood grouping of individual body fluids from a mixture of body fluids.…”

“…Preparation of P6-5H coated plate for sandwich ELISA was performed in the same way as described in the previous paper [2], except that purified P6-5H solution (2 gg/ml) was used for coating the plate. Specimens of mixed body fluids (50 gl) were added to the wells and incubated for 1 h at 37°C.…”

“…Preparation of P6-5H coated plate and incubation with specimens were performed as for sandwich ELISA. The absorption-elution test on the plate was carried out as described previously [2]. Although the amounts of seminal B G S varied between individual, they were detected to a dilution of at least 60,000 by E L I S A with P6-5H, irrespective of A B O blood group and secretor status (Fig.…”

“…Recently, we developed methods for ABO blood grouping of saliva from mixed body fluids by sandwich methods using monoclonal antibodies (mAbs) to a tissue-specific epitope (TSE) on the core protein of an ABO blood group substance (BGS) in saliva [1,2]. If seminal ABO-BGS also carries TSE on its core protein, ABO blood grouping of semen from mixed body fluids can be performed by sandwich methods using mAb to TSE of seminal ABO-BGS.…”