Dose-response relationship for the induction of chromosomal abnormalities in gamma-irradiated human spermatozoa

Alvarez, R.; Tusell, Laura; Miró, R.; Genescà, Anna; Caballı́n, Marı́a Rosa; Ribas, M.; Barquinero, Joan Francesc; Egozcue, J.

doi:10.1002/(sici)1098-2280(1997)29:4<357::aid-em3>3.0.co;2-7

Cited by 12 publications

(5 citation statements)

References 39 publications

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“…Similar observations concerning the erythrocytes of persons occupationally exposed to radiation were presented by Kłuciński et al [61]. The hazardous effect of gamma radiation was confirmed by the results obtained in the study by Alvarez et al carried out on male sperm, which showed the effect of this radiation on the occurrence of sperm DNA damage [62]. Similarly, the adverse effects of gamma radiation were described on the animal model by Saiyad Musthafa et al, who observed disorders of the endocrine function of male gonads in fish after irradiation [63].…”

Section: Conditioning Of Radiosensitivity Of Premeiotic Cells Cells supporting

confidence: 78%

Effect of ionizing radiation on the male reproductive system

Wdowiak¹,

Skrzypek²,

Stec³

et al. 2019

Ann Agric Environ Med.

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Introduction and objective. In the light of current data concerning the growing exposure to ionizing radiation (IR) originating from atrificial sources, especially from medical ones, and also related to occupational exposure, it is justifiable to systematize the state of knowledge concerning the effect of IR on the male reproductive system. Brief description of the state of knowledge. There is no basis for the application of the hypothesis of hormesis in the area of male reproductive health. Regarding the impact of IR on spermatogenesis, spermatogonia are less susceptible to the occurrence of DNA damage after exposition to IR, but are characterized by slower DNA repair compared to somatic cells. Damage to the genes after exposure to IR is possible at each stage of spermatogenesis; however, haploidal spermatids show the highest radiosensitivity in this respect. The genetic risk of the cells differentiating during spermatogenesis is limited to one cycle of spermatogenesis, whereas the genetic instability may persist for the whole period of life, and DNA damage induced by IR may be transmitted to future generations. The minimum dose causing detectable DNA damage was 30 Gy. While exceeding this dose, the number of single-strand DNA breaks increases. Among males exposed to IR, a decrease was observed in sperm motility and in the percentage of morphologically normal spermatozoa as well as in an intensification of vacuolization. The genetic material in the sperm of these males showed higher fragmentation and methylation of genomic DNA. Conclusion. In the context of the epidemiological situation concerning the prevalence of infertility, while assessing the health effects of exposure to IR from artificial, including medical sources, the reproductive risk should be considered.

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Section: Conditioning Of Radiosensitivity Of Premeiotic Cells Cells supporting

confidence: 78%

Effect of ionizing radiation on the male reproductive system

Wdowiak¹,

Skrzypek²,

Stec³

et al. 2019

Ann Agric Environ Med.

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“…1), showing the relative biological effectiveness (RBE) values of approximately 1. In the data of Brandriff et al [10] and Alvarez et al [13] (Table 1, a,e), however, the equations had a much slower gradient than ours.…”

Section: Chromosome Analysiscontrasting

confidence: 59%

“…The effects of radiation on human sperm chromosomes have been studied with five kinds of ionizing radiation, i.e., 137 Cs gamma-rays [10,11], 60 Co gamma-rays [12,13], X-rays [14], 3 H beta-rays [15], and 252 Cf fission neutrons [12]. In our gamma-ray experiment [11], the incidence of spermatozoa with radiation-induced structural chromosome aberrations (Y) increased exponentially with increasing dosage (D), being expressed by an equation, Y(%)=100 (1-e -0.514D ) ( Table 1, b and Fig.…”

Section: Chromosome Analysismentioning

confidence: 99%

Radiation- and chemical-induced structural chromosome aberrations in human spermatozoa

Kamiguchi

Tateno

2002

Mutation Research/Fundamental and Molecular Mechanisms of Mutag

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Previous studies on the clastogenic effects of mutagens on human sperm chromosomes were reviewed.A marked increase of structural chromosome aberrations has been reported in the spermatozoa irradiated in vitro with five kinds of ionizing radiation ( 137 Cs gamma-, 60 Co gamma-, X-, and 3 H beta-rays and 252 Cf neutrons). The micronucleus test with hybrid 2-cell embryos generated from human sperm and hamster oocytes was shown to be useful as a simple and rapid method for assessing the effects of radiation. Radiosensitivity of human spermatozoa was highest, being followed by golden hamster, Chinese hamster and mouse spermatozoa.Chromosome-damaging effects were also found with some chemicals (bleomycin, daunomycin, methyl methanesulfonate, triethylenemelamine, neocarzinostatin, N-methyl-N'-nitro-N-nitorosoguanidine, and mitomycin C), but not with other chemicals (urethane, nitrobenzene, dioxin, cyclophosphamide, benzo(a)pyrene, and N-nitrosodimethylamine). The clastogenicity of chemical metabolites was confirmed for cyclophosphamide and benzo(a)pyrene, by using the S9-based metabolic activation system. The results of sperm chromosome analysis from cancer patients who had undergone radio-and/or chemotherapy were contradictory among investigators and further studies are necessary. The importance of mutagenicity testing with human spermatozoa is discussed.

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“…In the current study, the most adverse implications on semen morphology were related to gamma radiation. The harmful effects of this radiation on male sperm were also confirmed by the results of a study by Alvarez R. et al who indicated that this kind of radiation has effects on the occurrence of sperm DNA damage [24]. Such a negative effect of gamma radiation has likewise been described in an animal model by Saiyad Musthafa M. et al After irradiation, they observed disorders in the endocrine function of male gonads in fish [25].…”

Section: Discussionmentioning

confidence: 54%

Background ionizing radiation and semen parameters of men with reproductive problems

Wdowiak

Stec²,

Raczkiewicz

et al. 2020

Ann Agric Environ Med.

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Introduction and objective. The male reproductive system constitutes a set of tissues which are particularly sensitive to external factors. The aim of the study was to analyze the relationship between background radioactivity and the quality of the semen of the men who have reported to the infertility treatment facility in 2000-2016 in the Lublin region of eastern Poland. Materials and method. The radioactivity of the ground-level air layer obtained from the Institute of Meteorology and Water Management in Włodawa in the Lublin region was analysed. 4,250 spermiograms of patients who reported to the treatment facility for the first time due to infertility were analysed and correlated to background radioactivity in the Lublin region in 2000-2016. Results. A long-term decrease in the percentage of morphologically normal spermatozoa of the patients was observed (r=-0.970; p<0.001 in 2000-2009 and r=-0.925; p=0.003 in 2010-2016). Men's age correlated negatively with sperm motility (r=-0.164; p=0.009) and morphology (r=-0.186; p=0.009). The percentage of spermatozoa with normal morphology was lowered by beta-isotopes of artificial origin in the air in 2000-2009 (r=-0.655; p=0.040) and by the exposure to gamma radiation of the ground-level atmosphere in 2010-2016 (r=-0.676; p=0.048). The percentage of sperm vitality was lowered by gamma radiation in the atmosphere (r=-0.636; p=0.006), but improved by beta isotopes in precipitation (r=0.686; p=0.002) in the whole of the analyzed period. Conclusions. The percentage of morphologically normal spermatozoa in patients who reported to the infertility treatment depends not only on the age of patient, but also on beta-isotopes of artificial origin and gamma radiation in the atmosphere. Beta isotopes in precipitation affect the improvement of sperm vitality.

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Dose-response relationship for the induction of chromosomal abnormalities in gamma-irradiated human spermatozoa

Cited by 12 publications

References 39 publications

Effect of ionizing radiation on the male reproductive system

Effect of ionizing radiation on the male reproductive system

Radiation- and chemical-induced structural chromosome aberrations in human spermatozoa

Background ionizing radiation and semen parameters of men with reproductive problems

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