Dose response of micronuclei induced by combination radiation of α-particles and γ-rays in human lymphoblast cells

Ren, Ruiping; He, Mingyuan; Dong, Chen; Xie, Yun; Ye, Shuang; Yuan, Daqing; Shao, Chunlin

doi:10.1016/j.mrfmmm.2012.12.007

Cited by 21 publications

(8 citation statements)

References 25 publications

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“…Although, biological responses to low-dose ionizing radiation are exhibited in several ways, adaptive response is considered one of them. [36] Induction of radio-adaptive response by low-level ionizing radiation was previously reported for protection against radiation induced chromosomal aberrations in human lymphocytes. [37] Our results could support the hypothesis that the radioresistance effects of low dose exposure may be due to the up-regulated expression of Bcl-2 genes that may provide a survival advantage.…”

Section: Resultsmentioning

confidence: 99%

Up-regulation of Bcl-2 expression in cultured human lymphocytes after exposure to low doses of gamma radiation

et al. 2015

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Lymphocytes have demonstrated complex molecular responses to induced stress by ionizing radiation. Many of these reactions are mediated through modifications in gene expressions, including the genes involved in apoptosis. The primary aim of this study was to assess the effects of low doses of ionizing radiation on the apoptotic genes, expression levels. The secondary goal was to estimate the time-effect on the modified gene expression caused by low doses of ionizing radiation. Mononuclear cells in culture were exposed to various dose values ranged from 20 to 100 mGy by gamma rays from a Cobalt-60 source. Samples were taken for gene expression analysis at hours 4, 24, 48, 72, and 168 following to exposure. Expression level of two apoptotic genes; BAX (pro-apoptotic) and Bcl-2 (anti-apoptotic) were examined by relative quantitative real-time polymerase chain reaction (PCR), at different time intervals. Radio-sensitivity of peripheral blood mononucleated cells (PBMCs) was measured by the Bcl-2/BAX ratio (as a predictive marker for radio-sensitivity). The non-parametric two independent samples Mann–Whitney U-test were performed to compare means of gene expression. The results of this study revealed that low doses of gamma radiation can induce early down-regulation of the BAX gene of freshly isolated human PBMCs; however, these changes were restored to near normal levels after 168 hours. In most cases, expression of the Bcl-2 anti-apoptotic gene was up-regulated. Four hours following to exposure to low doses of gamma radiation, apoptotic gene expression is modified, this is manifested as adaptive response. Modification of these gene expressions seems to be a principle pathway in the early radioresistance response. In our study, we found that these changes were temporary and faded completely within a week.

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Section: Resultsmentioning

confidence: 99%

Up-regulation of Bcl-2 expression in cultured human lymphocytes after exposure to low doses of gamma radiation

et al. 2015

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“…Application of ionizing radiation in many different fields is constantly increasing, including the use for medical purposes. Ionizing radiation is known to induce mutations and cell transformations, predominantly by causing single-strand and double-strand DNA breakage, thereby leading to chromosome instability and carcinogenesis [ 10 ]. Of all workers exposed to man-made sources of radiation, medical personnel represent the largest group, but receive relatively low doses.…”

Section: Discussionmentioning

confidence: 99%

New models for prediction of micronuclei formation in nuclear medicine department workers

Terzić¹,

Milovanović

Dotlić

et al. 2015

J Occup Med Toxicol

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BackgroundIonizing radiation causes detrimental health effects such as cancer and genetic damage. The study aim was to determine predictors for micronuclei (MN) occurrence and frequency in peripheral blood lymphocytes of health workers professionally exposed to radiation.MethodsHealth workers, age matched, selected for the study on regular check-ups, were divided according to the radiation exposure. The exposed group involved nuclear medicine department employees (54) and the control group comprised workers from other departments (36). Data about workers characteristics and habits, received annual doses (AD), total years of service (TYS) and exposed years of service (EYS) were taken from each subject. Blood samples were taken and micronuclei (MN) number in peripheral blood lymphocytes was calculated using CBMN assay according to standard protocols.ResultsMost workers were female, technicians, with mean age of 45.67 years and EYS about 15 years. Health workers exposed to radiation had significantly more MN than controls (p = 0.001). Female gender, older age, higher received annual doses, longer EYS and TYS increased the MN number. Technicians and laboratory workers have higher risk for MN occurrence. Significant predictors of MN formation according to constructed model were workers age, sex, AD and EYS. One EYS year increases MN frequency 1.017 times, while receiving 0.1 mSy raises MN frequency by 26 %. EYS accurately predicts 86.30 % of MN frequencies and AD 64.60 %.ConclusionsThe model, developed for the first time in this study, showed that received annual doses and duration of exposure to radiation can be used for prediction of MN numbers.

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“…To date, a great deal of studies proved the existence of RIBEs in vivo 4 5 and in vitro 6 7 . A set of RIBEs endpoints have been reported, including micronuclei 8 9 10 , gene mutation 11 12 13 , chromosomal aberration 14 , DNA damage 8 15 16 17 18 , apoptosis or cell killing 19 20 21 , inflammatory response 22 23 24 , etc. Recently, Wang et al .…”

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confidence: 99%

Bystander autophagy mediated by radiation-induced exosomal miR-7-5p in non-targeted human bronchial epithelial cells

Song

Wang

Shang

et al. 2016

Sci Rep

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Radiation-induced bystander effect (RIBE) describes a set of biological effects in non-targeted cells that receive bystander signals from the irradiated cells. RIBE brings potential hazards to adjacent normal tissues in radiotherapy, and imparts a higher risk than previously thought. Excessive release of some substances from irradiated cells into extracellular microenvironment has a deleterious effect. For example, cytokines and reactive oxygen species have been confirmed to be involved in RIBE process via extracellular medium or gap junctions. However, RIBE-mediating signals and intercellular communication pathways are incompletely characterized. Here, we first identified a set of differentially expressed miRNAs in the exosomes collected from 2 Gy irradiated human bronchial epithelial BEP2D cells, from which miR-7-5p was found to induce autophagy in recipient cells. This exosome-mediated autophagy was significantly attenuated by miR-7-5p inhibitor. Moreover, our data demonstrated that autophagy induced by exosomal miR-7-5p was associated with EGFR/Akt/mTOR signaling pathway. Together, our results support the involvement of secretive exosomes in propagation of RIBE signals to bystander cells. The exosomes-containing miR-7-5p is a crucial mediator of bystander autophagy.

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Dose response of micronuclei induced by combination radiation of α-particles and γ-rays in human lymphoblast cells

Cited by 21 publications

References 25 publications

Up-regulation of Bcl-2 expression in cultured human lymphocytes after exposure to low doses of gamma radiation

Up-regulation of Bcl-2 expression in cultured human lymphocytes after exposure to low doses of gamma radiation

New models for prediction of micronuclei formation in nuclear medicine department workers

Bystander autophagy mediated by radiation-induced exosomal miR-7-5p in non-targeted human bronchial epithelial cells

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