New models for prediction of micronuclei formation in nuclear medicine department workers

Terzić, Sanja; Milovanović, Aleksandar; Dotlić, Jelena; Rakić, Boban; Terzić, Milan

doi:10.1186/s12995-015-0066-5

Cited by 10 publications

(7 citation statements)

References 19 publications

(22 reference statements)

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“…Hospital workers occupationally exposed to low levels of ionizing radiation exhibit high frequency of chromosomal aberrations in peripheral lymphocytes (Maffei et al, 2004). Ionizing radiation causes detrimental health effects such as cancer and genetic damage (Terzic, Milovanovic, Dotlic, Rakic, & Terzic, 2015). Ionizing radiation induces mutations and cell transformations, predominantly by causing single-strand and double-strand DNA breakage, leading to chromosome instability and carcinogenesis (Eken et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Chromosomal aberrations and oxidative DNA adduct 8-hydroxy-2-deoxyguanosine as biomarkers of radiotoxicity in radiation workers

El-Benhawy

Sadek

Behery

et al. 2016

Journal of Radiation Research and Applied Sciences

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a b s t r a c tBackground: There are evidences of association between occupational radiation exposure, cytogenetic alterations and the increase in cancer rates. It is known that the probability of carcinogenesis is greater in populations exposed to radiation, since ionizing radiation can raise the frequency of chromosomal aberration and spontaneous mutations.Objective: Our purpose was to assess the role of chromosomal aberrations and oxidative DNA adduct 8-hydroxy-2-deoxyguanosine (8-OHdG) as biomarkers of radiation injury in individuals occupationally exposed to ionizing radiation.Subjects: and Methods: Blood samples were collected from 60 radiation workers and 30 healthy volunteers age and sex matched as control group who had never worked in radiation-related jobs. Chromosomal aberrations in peripheral blood lymphocytes were assayed by conventional cytogenetic technique and serum levels of 8-OHdG was measured by enzyme linked immunossorbent assay (ELISA).Results: The incidence of all types of chromosomal aberrations was significantly higher in all exposed groups than in controls with the highest rate of chromosomal aberrations in the industrial radiographers. Serum 8-OHdG in all radiation workers was significantly higher than in control group. There was a significant higher values among industrial radiographers compared to diagnostic radiologists or radiotherapists. Significantly lower mean corpuscular volume (M.C.V) was found among radiation workers versus the controls reflecting erythrocyte microcytosis.Conclusions: Scoring of chromosome aberrations such as breaks, fragments and dicentrics is a reliable method to detect previous exposure to ionizing radiation. This type of monitoring may be used as a biological dosimeter instead of physical dosimetry.8-OHdG is a useful oxidative DNA marker among radiation workers and those exposed to environmental carcinogens.

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Section: Discussionmentioning

confidence: 99%

Chromosomal aberrations and oxidative DNA adduct 8-hydroxy-2-deoxyguanosine as biomarkers of radiotoxicity in radiation workers

El-Benhawy

Sadek

Behery

et al. 2016

Journal of Radiation Research and Applied Sciences

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“…-These chemical contaminants in hospital complex are present in addition to the ionizing radiation; several chemical contaminants may have genotoxic effects, and thus, there is possibility that the higher MN in hospital subjects may be the cumulative effect of IR and chemical pollutants. Ionizing radiation induces significant health effects such as cancer and genetic damage (Terzic et al, 2015). Ionizing radiation causes mutations and cell transformations, predominantly by causing single-strand and double-strand DNA breakage, leading to chromosome instability and carcinogenesis (Eken et al, 2010)‖.…”

Section: Discussionmentioning

confidence: 99%

A cytogenic monitoring approach of hospital workers occuptionally exposed to ionizing radiations using micronucleus assay

Sultana¹,

Tabassum²,

Sultana³

et al. 2016

Afr. J. Trad. Compl. Alt. Med.

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Background: The objective of this study was to determine chromosomal damage in occupational workers of the radiation department from three different hospitals, Faisalabad, Pakistan exposed for a long term to ionizing radiations using micronucles (MN) assay. A comparison between exposed and non-exposed subjects (controlled) of same age exhibited a significant an increase in the number of micronuclei in occupational workers. MN frequency increases with an increase in age and duration of exposure in both sexes but higher in females. Materials and Methods:The study was conducted at the District Head Quarter Hospital (DHQ), Punjab Institute of Nuclear Medicine (PINUM) and Allied Hospital, Faisalabad, Pakistan. The total 145 subjects were selected from these hospitals. The subjects were divided into two groups. The control group (N= 40) (20 males and 20 females) of healthy subjects (no exposure) and the second group of subjects (N=105) (68 males and 37 females) subjects of occupational workers who were indirectly exposed to radiation. Blood samples (2ml) were collected in sodium heparinised vaccutainer tubes through venipuncture from both the groups. Disposable syringes were used for this purpose. For the evaluation of MN yield, slides were prepared by following the method of Jorge et al. (2004). Results: A significant difference in micro nuclear induction was observed between the occupational subjects and the control subjects and as well as in females and in males (P < 0.01). Females are more vulnerable to ionizing radiation than males. In females, MN yield was two times higher than males. MN frequency was increased with an increase in age and duration of exposure in both sexes, but higher in females and may be due to an increase in chromosomal loss in hospital workers. There is an individual response to the physical noxa, depending on sex, age and exposure. Smoking and drinking habits do not have a significant effect in increasing the number of MN in occupationally exposed workers. Conclusion: It was concluded that females are more vulnerable to ionizing radiations than males. MN test can be used as a biomarker with a predictive value for the estimation in occupationally exposed subjects.

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“…Siama et al (2019) registraron un aumento en la frecuencia de MN en un grupo de 33 tecnólogos de imagenología en comparación a un mismo número de personal no expuesto a RI; la formación de MN aumentaba conforme se incrementaba la edad del individuo y la cantidad de pacientes atendidos. Terzic et al (2015) encontraron que, en un grupo de 56 trabajadores de medicina nuclear, los profesionales en imagenología de sexo femenino, con mayor edad, con mayores dosis de exposición anuales y más número de años de exposición ocupacional, tienden a presentar mayor frecuencia de MN. Así mismo, Qian et al (2016) evidenciaron un aumento significativo de la frecuencia de MN en una muestra de 1392 trabajadores hospitalarios expuestos a RI con respecto a 143 no expuestos, al igual que una asociación positiva entre la frecuencia de MN y la dosis acumulada de exposición a RI.…”

Section: Conclusionesunclassified

Estudio comparativo de biomonitoreo citogenético en personal veterinario expuesto ocupacionalmente a radiaciones ionizantes mediante el ensayo de micronúcleos con bloqueo de la citocinesis

Chaves-Campos

Gamboa

Ramírez-Mayorga

et al. 2022

PSM

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Introducción: las radiaciones ionizantes (RI) pueden inducir la formación de micronúcleos (MN). La frecuencia de MN se utiliza como biomarcador de daño genético inducido por (RI). Objetivo: evaluar el daño al ADN resultante de la exposición ocupacional a RI en personal de clínicas veterinarias o afines. Metodología: se utilizó el ensayo de micronúcleos con bloqueo de la citocinesis (MNBC) para comparar la frecuencia observada del biomarcador en 40 individuos expuestos ocupacionalmente a RI con respecto a un grupo control de 32 participantes, ambos grupos pertenecen a personal veterinario. Además, se registraron variables demográficas, de estilo de vida y ocupacionales que pudieran influir en la formación de MN. Resultados: el análisis univariado no demostró diferencias significativas en la frecuencia de MN entre los grupos de estudio (p = 0,118). Mediante análisis multivariado se obtuvo que aproximadamente un 27 % (R2 ajustado = 0,269) de la variabilidad de la frecuencia de MN se explica por la influencia conjunta de la edad, el sexo y el número de radiografías realizadas. La edad es la variable de mayor importancia relativa (β = 0,504), seguida del sexo (β = -0,316) y el número de radiografías diarias (β = 0,214). Conclusiones: la frecuencia de MN tiende a aumentar en mujeres, a medida que aumenta la edad del participante y a mayor número de radiografías realizadas.

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New models for prediction of micronuclei formation in nuclear medicine department workers

Cited by 10 publications

References 19 publications

Chromosomal aberrations and oxidative DNA adduct 8-hydroxy-2-deoxyguanosine as biomarkers of radiotoxicity in radiation workers

Chromosomal aberrations and oxidative DNA adduct 8-hydroxy-2-deoxyguanosine as biomarkers of radiotoxicity in radiation workers

A cytogenic monitoring approach of hospital workers occuptionally exposed to ionizing radiations using micronucleus assay

Estudio comparativo de biomonitoreo citogenético en personal veterinario expuesto ocupacionalmente a radiaciones ionizantes mediante el ensayo de micronúcleos con bloqueo de la citocinesis

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