DOSE RATE DEPENDENCE OF MUTATION RATES FROM Γ-Irradiated POLLEN GRAINS OF MAIZE

Mabuchi, Tomoo; Matsumura, Seiji

doi:10.1266/jjg.39.131

Cited by 17 publications

(10 citation statements)

References 13 publications

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“…It was reported that on irradiating at the same dose, higher dose rates were more effective in inducing mutations than lower dose rates in oats (Nishiyama et al 1966) and in maize (Mabuchi and Matsumura 1964). Moreover, in saintpaulia (Broertijes 1968) and Tradescantia (Nauman et al 1975), it was observed that the dose rate effect occurred at higher doses, while little dose rate effect was observed at lower doses.…”

Section: Introductionmentioning

confidence: 99%

Effects of dose and dose rate of gamma ray irradiation on mutation induction and nuclear DNA content in chrysanthemum

et al. 2008

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We investigated the effect of total irradiation dose and dose rate on flower color mutation and nuclear DNA content as an index of radiation damage in chrysanthemum. Chrysanthemum morifolium cv. 'Taihei' plants grown by in vitro culture were gamma-irradiated with a total dose of 15, 30 and 60 Gy at a rate of 0.5, 1, 2 and 5 Gy/h. Leaf explants cut from the irradiated plants were tissue cultured, and the regeneration rates and frequency of flower color mutation were investigated. Nuclear DNA content was measured by flow cytometric analysis. The regeneration rate decreased with increase in the total dose and dose rate of irradiation. Mutation frequency did not differ significantly among dose rates, indicating that mutation frequency was independent of dose rate and was dependent mainly on total dose. Comparison of the average of the nuclear DNA content with each treatment revealed that it was influenced by both dose rate and total dose, and that the reduction in nuclear DNA content was less at low dose rates, even when total doses were high. It appears that same mutation frequencies were obtained without large reduction in nuclear DNA content by 0.5 Gy/h, when compared with 2 Gy/h. Consequently, we conclude that gamma ray irradiations of high total doses at low dose rates efficiently induce mutations with less radiation damage in chrysanthemum.

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Section: Introductionmentioning

confidence: 99%

Effects of dose and dose rate of gamma ray irradiation on mutation induction and nuclear DNA content in chrysanthemum

et al. 2008

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“…A low dose rate was reported to be more effective to induce mutations than a high dose rate in barley and maize [21]. However, Mabuchi and Matsumura [22] suggested that a high dose rate could induce a higher mutation frequency than a low dose rate under an identical total dose in maize. In contrast, the mutation frequency is dependent on the total dose, not the dose rate, in chrysanthemum [23].…”

Section: Optimal γ-Irradiation Condition For Mutation Inductionmentioning

confidence: 99%

“…Irradiation dose has been mainly used for determination of the optimal irradiation condition in diverse plant species, including orchids [5,8,[13][14][15][16][17][18]. However, the optimal doses suggested by previous researchers are diverse: e.g., LD 10 in rice seeds [18]; LD and RD in crop seeds [19]; LD [20][21][22][23][24][25][26][27][28][29][30] in in vitro tissues [20]; and RD 50 in Cymbidium protocorm-like bodies (PLBs) [9]. Furthermore, irradiation duration and dose rate, a complex concept of dose and duration, are also important factors for induction of mutations.…”

Section: Optimal γ-Irradiation Condition For Mutation Inductionmentioning

confidence: 99%

Frequency, Spectrum, and Stability of Leaf Mutants Induced by Diverse γ-Ray Treatments in Two Cymbidium Hybrids

Kim

Ahn

et al. 2020

Plants

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Ionizing radiation combined with in vitro tissue culture has been used for development of new cultivars in diverse crops. The effects of ionizing radiation on mutation induction have been analyzed on several orchid species, including Cymbidium. Limited information is available on the comparison of mutation frequency and spectrum based on phenotypes in Cymbidium species. In addition, the stability of induced chimera mutants in Cymbidium is unknown. In this study, we analyzed the radiation sensitivity, mutation frequency, and spectrum of mutants induced by diverse γ-ray treatments, and analyzed the stability of induced chimera mutants in the Cymbidium hybrid cultivars RB003 and RB012. The optimal γ-irradiation conditions of each cultivar differed as follows: RB003, mutation frequency of 4.06% (under 35 Gy/4 h); RB012, 1.51% (20 Gy/1 h). Re-irradiation of γ-rays broadened the mutation spectrum observed in RB012. The stability of leaf-color chimera mutants was higher than that of leaf-shape chimeras, and stability was dependent on the chimera type and location of a mutation in the cell layers of the shoot apical meristem. These results indicated that short-term γ-irradiation was more effective to induce mutations in Cymbidium. Information on the stability of chimera mutants will be useful for mutation breeding of diverse ornamental plants.Plants 2020, 9, 546 2 of 11 breeding [6]. However, development of a new Cymbidium cultivar by means of cross-breeding is time-consuming because of the long vegetative growth stage.Mutation breeding techniques using physical mutagens (e.g., γ-rays, X-rays, and ion particles) or chemical mutagens (e.g., ethyl methanesulfonate, N-nitroso-N-methylurea, and colchicine) have been widely used to develop mutant cultivars in diverse plant species, including food and ornamental crops. In 226 plant species, 3308 mutants, including two Cymbidium mutants, that were predominantly (77.5%) developed using physical mutagens have been registered in the Mutant Variety Database of the joint Food and Agriculture Organization of the United Nations/International Atomic Energy Agency [7]. Among the diverse ornamental plants registered on the database, the most numerous mutants are those of chrysanthemum (Chrysanthemum spp., 274 mutants) followed by rose (Rosa spp., 67 mutants), dahlia (Dahlia spp., 36 mutants), alstroemeria (Alstroemeria spp., 35 mutants), streptocarpus (Streptocarpus spp., 30 mutants), and carnation (Dianthus caryophyllus, 28 mutants) [7]. The combination of treatment with a physical mutagen and in vitro tissue culture has been used to shorten the breeding period of orchids [8,9]. Thus, integration of γ-irradiation and in vitro tissue culture may be an efficient procedure for development of mutant cultivars in Cymbidium.The treatment of seeds, buds (tip/node cuttings), callus, and rhizomes with a mutagen induces chimeras in M 1 plants because mutations are induced in individual cells and regenerated shoots are recovered from pre-existing multicellular meristems [10]. In seed-propagated pl...

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“…Most studies in literature have given attention to the total dose, and only a few studies have emphasized the dose rate. Among those few studies, we applied our mathematical model to the data of five species of animals (mice and drosophila) and plants (maize, Tradescantia, and chrysanthemum) 2,[8][9][10][11] . With experimental subjects and the irradiation rate (assumed to be constant), we determined the 4 parameters via an χ 2 test, convert data with different dose rates and the total dose to scaling functions, and compared with the experimental data.…”

Section: Comparison With Experimental Datamentioning

confidence: 99%

The Reconsider Linear Non-Threshold (LNT) Hypothesis of Radiation Effect on Living Object

Manabe

Nakamura

Nakajima

et al. 2021

Insights Concerning the Fukushima Daiichi Nuclear Accident Vol. 1

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After Muller's experiment on drosophila, it was found that the risk of radiation depends on the total dose, and the linear-non-threshold (LNT) hypothesis became the basis of radiation protection. However, Russel et al. later showed the existence of the dose rate effect. We propose a mathematical model for integrating the results for different species and extrapolating them to humans. With scaling rule, the model can help interpret various data in a comprehensive manner. This would produce new knowledge in radiation risk assessment.

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DOSE RATE DEPENDENCE OF MUTATION RATES FROM Γ-Irradiated POLLEN GRAINS OF MAIZE

Cited by 17 publications

References 13 publications

Effects of dose and dose rate of gamma ray irradiation on mutation induction and nuclear DNA content in chrysanthemum

Effects of dose and dose rate of gamma ray irradiation on mutation induction and nuclear DNA content in chrysanthemum

Frequency, Spectrum, and Stability of Leaf Mutants Induced by Diverse γ-Ray Treatments in Two Cymbidium Hybrids

The Reconsider Linear Non-Threshold (LNT) Hypothesis of Radiation Effect on Living Object

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