Dose-Dependent Hematological, Hepatic and Gonadal Toxicity of Cypermethrin in Wistar Rats

Das, Tuhina; Ghosh, Rita; Paramanik, Anurag; Pradhan, Ananya; Dey, Sumit; Roy, Tamanna; Chatterjee, Debjani; Choudhury, Sujata Maiti

doi:10.17140/tfmoj-2-122

Cited by 9 publications

(6 citation statements)

References 28 publications

(40 reference statements)

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“…And as can be seen from the data presented, pyrethroids have endocrine-disruptive properties when the mammalian body is exposed to them, causing disturbances in various organs and systems, including the reproductive system (Perry et al, 2007;Jin et al, 2010;Zhao et al, 2010). The endocrine-disruptive properties of pyrethroids are associated with a dysfunction of many nuclear and hormonal membrane receptors, which ultimately leads to pathological changes in the function of the gonads and the reproduction processes of offspring (Du et al, 2010;Kim et al, 2015;Das et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Comparative analysis of two methodological approaches to the study of endocrine disruptor alpha-cypermethrin reproductive toxicity

Shepelska

Prodanchuk

Kolianchuk

2021

Regul. Mech. Biosyst.

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At present, one of the main threats to humanity is undoubtedly endocrine disruptors (ED), since they directly disrupt the processes of ensuring homeostasis, which are identical to the very essence of the concept of health, considered in valeology as the ability to maintain age-appropriate stability in conditions of sharp changes in quantitative and qualitative parameters of the triune flow of sensory, verbal and structural information. Pesticides can disrupt the physiological functioning of many endocrine chains, including the endocrine mechanisms that ensure reproductive health. The study aimed to compare the results of our studies of alpha-cypermethrin reproductive toxicity in the test system for studying gonadotoxic activity with data obtained in the test system "Three Generation Reproduction Study". The studies were performed on male and female Wistar Han rats with two generic samples of alpha-cypermethrin from different manufacturers at doses of 0.2, 1.0 and 3.0 mg/kg body weight. The exposure lasted 11 (males) and 10 (females) weeks. During the last two weeks of exposure the state of the estrous cycle, duration and frequency of each stage in females was studied. After the end of treatment functional parameters of the state of the gonads in males and the ability of animals to reproduce were examined. In males, the total sperm count, the absolute and relative number of motile germ cells, and the number of pathologically altered forms were evaluated. The results of the study showed that exposure of male and female Wistar Han rats to alpha-cypermethrin at doses of 0.2–3.0 mg/kg during gametogenesis had a toxic effect on the reproductive system characterized by impaired gonadal and reproductive functions. In our studies, alpha-cypermethrin was found to have reproductive toxicity (reduced number of corpora luteum and live fetuses, increased absolute and relative postimplantation death, reduced average weight of fetuses and litters) and endocrine-disruptive effect, having a pronounced antiandrogenic effect on males. Obvious signs of endocrine reproductive disorders (changes in testis and epididymis weight, deterioration in semen parameters, altered length of separate stages of the estrous cycle) were observed in both females and males. However, studies in a test system of three-generations did not reveal a reproductive and endocrine-disruptive effect of cypermethrin, the toxicity of which was recognized as equivalent to the toxicity of alpha-cypermethrin. The obtained results showed higher sensitivity, informative and diagnostic significance of the methods for studying gonadotoxicity than the methodology of reproductive toxicity studies in the test system “Three Generation Reproduction Study”.

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Section: Discussionmentioning

confidence: 99%

Comparative analysis of two methodological approaches to the study of endocrine disruptor alpha-cypermethrin reproductive toxicity

Shepelska

Prodanchuk

Kolianchuk

2021

Regul. Mech. Biosyst.

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“…Pyrethroids insecticides, synthetic derivatives of pyrethrins, are utilized worldwide because of their high toxicity to insects and minimal efficiency in mammals (Das et al, 2017;Tang et al, 2018). β-cyfluthrin (β-Cyf), a photostable type II pyrethroid insecticide, is widely utilized in agriculture and household applications (Wang et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Ocimum basilicum Essential Oil Modulates Hematotoxicity, Oxidative Stress, DNA Damage, and Cell Cycle Arrest Induced by β-cyfluthrin in Rat Liver

Jebur

El‐Sayed²,

El‐Demerdash³

2022

Front. Pharmacol.

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Pesticides are used in large quantities infrequently, resulting in environmental damage and health issues. The goal of the current study was to explore the ameliorating effect of Ocimum basilicum (Basil) leaves essential oil versus the harmful effects of β-cyfluthrin in rat liver. Male Wistar rats were classified at random into four groups; negative control (corn oil), basil leaves essential oil (BEO, 3 ml/kg), β-cyfluthrin (positive control) (β-Cyf; 15 mg/kg BW, 1/25 LD50), and BEO plus β-Cyf, respectively. The rats were given their doses orally every day for a month. Results revealed that BEO yielded 6.32 mg/g with 33 identified components, representing 97% of the total oil. BEO implicated a considerable level of total phenolic contents, DPPH radical scavenging capacity, ABTS activity, and FRAP. The treatment of β-Cyf dramatically elevated lipid peroxidation (TBARS and H2O2) (LPO), protein oxidation (PC, AOPP, and HYP), and considerably reduced enzymatic (SOD, CAT, GPx, GR, and GST) and non-enzymatic (GSH) antioxidants. After β-Cyf treatment, hematological parameters, body and liver weights, enzyme activity (AST, ALT, ALP, and LDH), as well as protein, albumin, globulin, and total bilirubin levels were all considerably affected. Furthermore, β-Cyf increased the expression of pro-inflammatory genes (TNF-α, IL-6) as well as DNA damage and cell cycle arrest in the G0/G1 phase and decreased the number of cells in S and G2/M phase of liver cells. Moreover, rats given BEO then intoxicated with β-Cyf showed substantial changes in the majority of the parameters tested. Finally, BEO was shown to have high antioxidant efficacy in combating β-Cyf toxicity because of its high phenolic content.

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“…The broad use of cypermethrin leads to its entry into water resources and provokes detrimental effects on humans and other organisms. The adverse effects include neurological, genetic and hematological toxicities, generation of reactive oxygen species, and toxic alterations in the liver, kidneys and reproductive organs [5][6][7][8][9]. Jamun (Syzygium cumini) has antidiabetic, antimalarial, antibacterial, free radical scavenging, anti-ulcer and antifertility properties [4,10,11].…”

Section: Introductionmentioning

confidence: 99%

The Protective Effects of Jamun Seeds and Orange Peels Extracts on Calcitonin Cells and Parathyroid Glands against Cypermethrin Toxicity

Srivastava

Srivastava²,

Srivastav

et al. 2021

IJT

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Background: This study aimed to investigate changes in Calcitonin Cells (C cells) and Parathyroid Glands (PTG) induced by cypermethrin exposure in rats and evaluate the protective effects of Jamun Seeds (Syzygium cumini; JSE) and orange peels (Citrus sinensis; OPE) extracts. Methods: Wistar rats (N=120) were treated in groups of 20 each as: A. Controls; B. Cypermethrin (CY); C. Cypermethrin and JSE; D. Cypermethrin and OPE; E. OPE; F. JSE. Thyro-parathyroid tissue samples were fixed on days 15 or 30 following each treatment. Results: Cypermethrin treatment in rats either alone or combined with JSE or OPE caused degranulation of calcitonin cells and mitochondrial enlargement on day 15 of exposure. Nuclear volume of C cells in groups B, C and D remained unchanged. On day 30, we found increased nuclear volumes, accumulation of secretory granules and degeneration of C cells. In groups E and F no changes in the morphology of C cells were observed. The PTG cells treated with cypermethrin or CY+JSE or CY+OPE over 15 days exhibited hyperchromatic, elongated and increased in volume of the nuclei. The nuclei of PTG cells treated with cypermethrin, CY+JSE or CY+OPE were hyperchromatic and elongated on day 30. Degenerate nuclei were detected after cypermethrin treatment, and the nuclear volumes were increased on day 30. In groups E and F there were no changes in PTG cells on days 15 and 30 post treatment. Conclusion: Cypermethrin provoked alterations in the calcitonin and PTG cells, with microscopic evidence of protection after treatment with JSE and OPE.

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Dose-Dependent Hematological, Hepatic and Gonadal Toxicity of Cypermethrin in Wistar Rats

Cited by 9 publications

References 28 publications

Comparative analysis of two methodological approaches to the study of endocrine disruptor alpha-cypermethrin reproductive toxicity

Comparative analysis of two methodological approaches to the study of endocrine disruptor alpha-cypermethrin reproductive toxicity

Ocimum basilicum Essential Oil Modulates Hematotoxicity, Oxidative Stress, DNA Damage, and Cell Cycle Arrest Induced by β-cyfluthrin in Rat Liver

The Protective Effects of Jamun Seeds and Orange Peels Extracts on Calcitonin Cells and Parathyroid Glands against Cypermethrin Toxicity

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