Ocimum basilicum Essential Oil Modulates Hematotoxicity, Oxidative Stress, DNA Damage, and Cell Cycle Arrest Induced by β-cyfluthrin in Rat Liver

Abstract

Pesticides are used in large quantities infrequently, resulting in environmental damage and health issues. The goal of the current study was to explore the ameliorating effect of Ocimum basilicum (Basil) leaves essential oil versus the harmful effects of β-cyfluthrin in rat liver. Male Wistar rats were classified at random into four groups; negative control (corn oil), basil leaves essential oil (BEO, 3 ml/kg), β-cyfluthrin (positive control) (β-Cyf; 15 mg/kg BW, 1/25 LD50), and BEO plus β-Cyf, respectively. The rats were given their doses orally every day for a month. Results revealed that BEO yielded 6.32 mg/g with 33 identified components, representing 97% of the total oil. BEO implicated a considerable level of total phenolic contents, DPPH radical scavenging capacity, ABTS activity, and FRAP. The treatment of β-Cyf dramatically elevated lipid peroxidation (TBARS and H2O2) (LPO), protein oxidation (PC, AOPP, and HYP), and considerably reduced enzymatic (SOD, CAT, GPx, GR, and GST) and non-enzymatic (GSH) antioxidants. After β-Cyf treatment, hematological parameters, body and liver weights, enzyme activity (AST, ALT, ALP, and LDH), as well as protein, albumin, globulin, and total bilirubin levels were all considerably affected. Furthermore, β-Cyf increased the expression of pro-inflammatory genes (TNF-α, IL-6) as well as DNA damage and cell cycle arrest in the G0/G1 phase and decreased the number of cells in S and G2/M phase of liver cells. Moreover, rats given BEO then intoxicated with β-Cyf showed substantial changes in the majority of the parameters tested. Finally, BEO was shown to have high antioxidant efficacy in combating β-Cyf toxicity because of its high phenolic content.

Assessment of anticancer, antimicrobial, antidiabetic, anti-obesity and antioxidant activity of Ocimum Basilicum seeds essential oil from Palestine

“…O. basilicum essential oil demonstrated a higher content of phenols and maximal levels of DNA protection and free radical scavenging against toxicity induced by cadmium chloride [ 46 ]. As found in other research, the antioxidant property of this plant oil may be due to the polyphenoid rosmarinic acid, which is a derivative of cinnamic acid [ 47 ].…”

Punica granatum (Pomegranate) Peel Extract Pre-Treatment Alleviates Fenpropathrin-Induced Testicular Injury via Suppression of Oxidative Stress and Inflammation in Adult Male Rats

“…Pyrethroids have been demonstrated to damage cells via oxidation, and to accumulate peroxidation products, which are employed as indicators of oxidative stress [ 8 , 54 ]. The testes are frequently exposed to low oxygen levels as a result of their constrained vascularity.…”

The role of stimulator of interferon genes‐mediated AMPK/mTOR/P70S6K autophagy pathway in cyfluthrin‐induced testicular injury

“…Therefore, pyrethroid pesticides could damage the testis. Some studies have found that cyfluthrin could cause abnormal morphology and death of spermatozoa 9 . Meanwhile, lambda cyhalothrin can also cause the decrease of sperm motility in rats with abnormal testicular morphology 35 .…”

“…In the early stage, the research group detected pesticide residues in vegetable greenhouse in the suburbs of this area, and found that the detection rate of cyfluthrin was the highest. Studies have shown that cyfluthrin exposure can cause neurological and reproductive system damage 9–11 . Therefore, the toxicity of cyfluthrin has aroused widespread concern.…”