Dose-Dependent Effect of Bradykinin on Muscular Blood Flow and Glucose Uptake in Man

Abstract

Using the forearm technique, the effect of bradykinin on muscular blood flow and glucose uptake in healthy man in the postabsorptive state (n = 8) was studied at different doses of an intra-arterial infusion of bradykinin (2.5-150 ng/min). The blood flow of the forearm was increased dose-dependently from basal 2.8 ± 0.3 up to 14.7 ± 2.8 m//(100 g x min). At lower bradykinin concentrations (2.5-25 ng/min), muscular glucose uptake was raised parallel to the increased blood flow from basal 0.71 ± 0.30 to 2.93 ± 0.50 μηιοΙ/OOOg χ min). However, at higher doses (50-150 ng/min) glucose uptake was decreased again. Thus, the greatest metabolic effect of bradykinin was seen at a calculated bradykinin concentration of approximately 1 χ 10~9M in the blood. Dosisabh ngige Wirkung von Bradykinin auf Durchblutung und Glucoseaufnahme der Skelettmuskulatur beim MenschenZusammenfassung: Mit Hilfe der Unterarmtechnik wurde die Wirkung von intraarteriell infundiertem Bradykinin in steigender Dosierung (2.5-150 ng/min) auf Durchblutung und Glucoseaufnahme des Skelettmuskels bei gesunden, n chternen M nnern (n = 8) untersucht. Dabei fand sich ein allm hlicher Anstieg des Blutflusses von 2.8 ± 0.3 auf 14.7 ± 2.8 m//(100 g χ min) parallel zur zunehmenden Bradykinindosis. Die muskul re Glucoseaufnahme stieg ebenfalls schrittweise von 0.71 ± 0.30 auf 2.93 ± 0.50 Mmol/( 100 g χ min) im unteren Bradykinin-Dosierungsbereich von 2.5-25 ng/min. Bei Infusion h herer Bradykininmengen (50-150 ng/min) nahm die Glucoseutilisation jedoch wieder aufwerte um 1.8 μηιοΙ/(100 g χ min) ab. Der gr te Stoffwechseleffekt des Kinins fand sich somit bei einer kalkulierten Bradykininkonzentration von etwa l χ 10~9M im Blut.

Association between angiotensin‐converting enzyme gene polymorphisms and exercise performance in patients with COPD

“…In addition, DD subjects demonstrate a significant increase in bradykinin degradation with an inverse relationship between the half‐life of bradykinin and serum ACE activity. These are intriguing effects because skeletal muscle cells contain a complete kallikrein‐kinin system and bradykinin is an endothelial‐dependent vasodilator that has important effects on substrate metabolism, stimulating glucose extraction from the extracellular fluid 22 . Besides, the ACE gene may influence the working efficiency of peripheral skeletal muscle by blocking the expression of inflammatory factors.…”

The vasodilatory actions of insulin on resistance and terminal arterioles and their impact on muscle glucose uptake

“…In one study, methacholine was infused without insulin into hypertensive subjects and significantly increased flow and doubled muscle glucose uptake 32. Studies testing another endothelium‐dependent vasodilator, bradykinin, in the absence of infused insulin have found contrasting results, showing either no change 10 or increased 33 glucose uptake. In the former, healthy subjects were used, and local bradykinin infusion into the leg doubled blood flow without altering leg glucose uptake.…”

Bradykinin type 2 receptor -9/-9 genotype is associated with triceps brachii muscle hypertrophy following strength training in young healthy men

“…Previous work showed that bradykinin could increase glucose uptake in the skeletal muscle during exercise [9,27]. Activation of bradykinin type 2 receptor could also result in an increased concentration of inositol triphosphate, which gives rise to increased calcium concentration within the cell [28,29].…”

“…Within skeletal muscle, bradykinin promotes glucose uptake and alters muscle blood flow [9-12]. Bradykinin exerts many of its effects by binding to bradykinin type 2 receptor ( B2BRK ) [13].…”