Dose dependency of time to death in single and mixed infections with a wildtype and egt deletion strain of Helicoverpa armigera nucleopolyhedrovirus

Georgievska, Liljana; Hoover, Kelli; Werf, Wopke Van der; Muñoz, Delia; Caballero, Primitivo; Cory, Jenny S.; Vlak, Just M.

doi:10.1016/j.jip.2010.01.008

Cited by 22 publications

(20 citation statements)

References 40 publications

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“…A fibroblast growth factor (FGF, Busu114) aids virus dissemination through the tracheal system [48], [49]. The egt gene which prevents larvae molting and pupation [50], [51] was found in BusuNPV (Busu124) [15] and the baculovirus with deficiency egt gene kill the infected larvae faster than wild type stains [52], [53]. BusuNPV also contains a sod (Busu92) and three iap genes ( iap-1 , Busu48; iap-2 , Busu54; and iap-3, Busu93).…”

Section: Resultsmentioning

confidence: 99%

Genome Sequence and Analysis of Buzura suppressaria Nucleopolyhedrovirus: A Group II Alphabaculovirus

Zhu

Yin

et al. 2014

PLoS ONE

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The genome of Buzura suppressaria nucleopolyhedrovirus (BusuNPV) was sequenced by 454 pyrosequencing technology. The size of the genome is 120,420 bp with 36.8% G+C content. It contains 127 hypothetical open reading frames (ORFs) covering 90.7% of the genome and includes the 37 conserved baculovirus core genes, 84 genes found in other baculoviruses, and 6 unique ORFs. No typical baculoviral homologous repeats (hrs) were present but the genome contained a region of repeated sequences. Gene Parity Plots revealed a 28.8 kb region conserved among the alpha- and beta-baculoviruses. Overall comparisons of BusuNPV to other baculoviruses point to a distinct species in group II Alphabaculovirus.

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Section: Resultsmentioning

confidence: 99%

Genome Sequence and Analysis of Buzura suppressaria Nucleopolyhedrovirus: A Group II Alphabaculovirus

Zhu

Yin

et al. 2014

PLoS ONE

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“…However, we failed to detect any HaNPV baculovirus in any of the 361 adults sampled. Only the larval stage is susceptible to baculovirus infection and so one possible explanation for this is that most of the baculovirus-infected individuals are lost from the system before adulthood due to increased larval mortality, abnormal pupation, or unsuccessful eclosion [48]. Alternatively, enhanced resistance to HaNPV in the adult stage may effectively clear all viral infections gained in the larval stage.…”

Section: Discussionmentioning

confidence: 99%

Densovirus Is a Mutualistic Symbiont of a Global Crop Pest (Helicoverpa armigera) and Protects against a Baculovirus and Bt Biopesticide

et al. 2014

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Mutualistic associations between symbiotic bacteria and their hosts are common within insect systems. However, viruses are often considered as pathogens even though some have been reported to be beneficial to their hosts. Herein, we report a novel densovirus, Helicoverpa armigera densovirus-1 (HaDNV-1) that appears to be beneficial to its host. HaDNV-1 was found to be widespread in wild populations of H. armigera adults (>67% prevalence between 2008 and 2012). In wild larval populations, there was a clear negative interaction between HaDNV-1 and H. armigera nucleopolyhedrovirus (HaNPV), a baculovirus that is widely used as a biopesticide. Laboratory bioassays revealed that larvae hosting HaDNV-1 had significantly enhanced resistance to HaNPV (and lower viral loads), and that resistance to Bacillus thuringiensis (Bt) toxin was also higher at low doses. Laboratory assays indicated that the virus was mainly distributed in the fat body, and could be both horizontally- and vertically-transmitted, though the former occurred only at large challenge doses. Densovirus-positive individuals developed more quickly and had higher fecundity than uninfected insects. We found no evidence for a negative effect of HaDNV-1 infection on H. armigera fitness-related traits, strongly suggesting a mutualistic interaction between the cotton bollworm and its densovirus.

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“…As a proof of concept, we have targeted the egt locus. Previous studies have revealed the potential of truncation of this gene for the improvement of the insecticidal properties of baculovirus (X. Chen et al, ; Cory et al, ; Georgievska et al, ; O'Reilly & Miller, ; Simón, Williams, López‐Ferber, & Caballero, ). Our egt knockout has shown similar levels of improvement as previous studies with other egt knockout mutants generated by insertion on the egt locus of a selecting marker (Cory et al, ; Georgievska et al, ; O'Reilly & Miller, ) and consequently considered as GM virus.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have revealed the potential of truncation of this gene for the improvement of the insecticidal properties of baculovirus (X. Chen et al, ; Cory et al, ; Georgievska et al, ; O'Reilly & Miller, ; Simón, Williams, López‐Ferber, & Caballero, ). Our egt knockout has shown similar levels of improvement as previous studies with other egt knockout mutants generated by insertion on the egt locus of a selecting marker (Cory et al, ; Georgievska et al, ; O'Reilly & Miller, ) and consequently considered as GM virus. From an applied point of view, and although certain countries are currently debating about the consideration of CRISPR/Cas9 mutants as GM or not (Gelinsky & Hilbeck, ; Shew, Nalley, Snell, Nayga, & Dixon, ), our results provide new tools for the generation of GM‐free virus with improved properties and potential use in the field.…”

Section: Discussionmentioning

confidence: 99%

Improvement of baculovirus as protein expression vector and as biopesticide by CRISPR/Cas9 editing

Pazmiño-Ibarra

Mengual-Martí

Targovnik

et al. 2019

Biotech & Bioengineering

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The clustered regularly interspaced short palindromic repeats (CRISPR) system–associated Cas9 endonuclease is a molecular tool that enables specific sequence editing with high efficiency. In this study, we have explored the use of CRISPR/Cas9 system for the engineering of baculovirus. We have shown that the delivering of Cas9‐single guide RNA ribonucleoprotein (RNP) complex with or without DNA repair template into Sf21 insect cells through lipofection might be efficient to produce knockouts as well as knock‐ins into the baculovirus. To evaluate potential application of our CRISPR/Cas9 method to improve baculovirus as protein expression vector and as biopesticide, we attempted to knockout several genes from a recombinant AcMNPV form used in the baculovirus expression system as well as in a natural occurring viral isolate from the same virus. We have additionally confirmed the adaptation of this methodology for the generation of viral knock‐ins in specific regions of the viral genome. Analysis of the generated mutants revealed that the editing efficiency and the type of changes was variable but relatively high. Depending on the targeted gene, the editing rate ranged from 10% to 40%. This study established the first report revealing the potential of CRISPR/Cas9 for genome editing in baculovirus, contributing to the engineering of baculovirus as a protein expression vector as well as a biological control agent.

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Dose dependency of time to death in single and mixed infections with a wildtype and egt deletion strain of Helicoverpa armigera nucleopolyhedrovirus

Cited by 22 publications

References 40 publications

Genome Sequence and Analysis of Buzura suppressaria Nucleopolyhedrovirus: A Group II Alphabaculovirus

Genome Sequence and Analysis of Buzura suppressaria Nucleopolyhedrovirus: A Group II Alphabaculovirus

Densovirus Is a Mutualistic Symbiont of a Global Crop Pest (Helicoverpa armigera) and Protects against a Baculovirus and Bt Biopesticide

Improvement of baculovirus as protein expression vector and as biopesticide by CRISPR/Cas9 editing

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