Genome Sequence and Analysis of Buzura suppressaria Nucleopolyhedrovirus: A Group II Alphabaculovirus

Zhu, Zheng; Yin, Feifei; Li, Xiaoping; Hou, Dianhai; Wang, Jun; Zhang, Lei; Arif, Basil M.; Wang, Hualin; Deng, Fei; Hú, Zhìhóng

doi:10.1371/journal.pone.0086450

Cited by 24 publications

(22 citation statements)

References 58 publications

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“…ORFs containing less than 50 amino acids were filtered out. Gene-parity plot analysis was conducted as previously described (Hu et al 1998a;Zhu et al 2014). Gene annotation and comparisons and detection of duplicate sequences were performed using NCBI's BLAST algorithm (http://blast.ncbi.nlm.nih.gov/Blast.cgi).…”

Section: Bioinformatics Analysismentioning

confidence: 99%

Genome Characteristics of the Cyclophragma Undans Nucleopolyhedrovirus: A Distinct Species in Group I of Alphabaculovirus

Zhu

Wang

et al. 2018

Virol. Sin.

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The Cyclophragma undans nucleopolyhedrovirus (CyunNPV), a potential pest control agent, was isolated from Cyclophragma undans (Lepidoptera: Lasiocampidae), an important forest pest. In the present study, we performed detailed genome analysis of CyunNPV and compared its genome to those of other Group I alphabaculoviruses. Sequencing of the CyunNPV genome using the Roche 454 sequencing system generated 142,900 bp with a G + C content of 45%. Genome analysis predicted a total of 147 hypothetical open reading frames comprising 38 baculoviral core genes, 24 lepidopteran baculovirus conserved genes, nine Group I Alphabaculovirus conserved genes, 71 common genes, and five genes that are unique to CyunNPV. In addition, the genome contains 13 homologous repeated sequences (hrs). Phylogenetic analysis groups CyunNPV under a distinct branch within clade "a" of Group I in the genus Alphabaculovirus. Unlike other members of Group I, CyunNPV harbors only nine of the 11 genes previously determined to be specific to Group I viruses. Furthermore, the CyunNPV lacks the tyrosine phosphatase gene and the ac30 gene. The CyunNPV F-like protein contains two insertions of continuous polar amino acids, one at the conventional fusion peptide and a second insertion at the pre-transmembrane domain. The insertions are likely to affect the fusion function and suggest an evolutionary process that led to inactivation of the F-like protein. The above findings imply that CyunNPV is a distinct species under Group I Alphabaculovirus.

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Section: Bioinformatics Analysismentioning

confidence: 99%

Genome Characteristics of the Cyclophragma Undans Nucleopolyhedrovirus: A Distinct Species in Group I of Alphabaculovirus

Zhu

Wang

et al. 2018

Virol. Sin.

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“…These viral species are phylogenetically distant to EpapGV [7]. We collinear genomic region for lepidopteran-infecting baculoviruses [30].…”

Section: Conservation Of Structural Proteins In the Family Baculoviridaementioning

confidence: 99%

Protein composition of the occlusion bodies of Epinotia aporema granulovirus

Masson

Fabre

Ferrelli

et al. 2018

Preprint

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Within family Baculoviridae, members of the Betabaculovirus genus are employed as biocontrol agents against lepidopteran pests, either alone or in combination with selected members of the Alphabaculovirus genus. Epinotia aporema granulovirus (EpapGV) is a fast killing betabaculovirus that infects the bean shoot borer (E. aporema) and is a promising biopesticide. Because occlusion bodies (OBs) play a key role in baculovirus horizontal transmission, we investigated the composition of EpapGV OBs. Using mass spectrometry-based proteomics we could identify 56 proteins that are included in the OBs during the final stages of larval infection. Our data provides experimental validation of several annotated hypothetical coding sequences. Proteogenomic mapping against genomic sequence detected a previously unannotated ac110-like core gene and a putative translation fusion product of ORFs epap48 and epap49. Comparative studies of the proteomes available for the family Baculoviridae highlight the conservation of core gene products as parts of the occluded virion. Two proteins specific for betabaculoviruses (Epap48 and Epap95) are incorporated into OBs.Moreover, quantification based on emPAI values showed that Epap95 is one of the most abundant components of EpapGV OBs.

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“…Baculoviridae (Ferrelli et al, 2012). No entanto, regiões hrs não foram encontradas no genoma de ChinNPV, como também relatado em Buzura supressaria NPV (BusuNPV), ChchNPV, TnSNPV e Agrotis segetum GV (AgseGV) (Faulkner et al, 1997;Willis et al, 2005;Hilton e Winstanley, 2008;Zhu et al, 2014).…”

Section: Homologous Regions (Hrs) São Ausentes No Genoma De Chinnpv-ieunclassified

Genoma e diversidade genética de populações de Chrysodeixis includens nucleopolyhedrovirus (ChinNPV)

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Aos meus queridos familiares, pais, irmãos, avós, cunhados, sobrinhos, sogros, tios e primos pela preocupação demonstrada durante toda a realização do trabalho e pelo carinho, amor e dedicação proporcionados todos esses anos. À minha maravilhosa família construída ao lado do meu querido marido Fernando C. Fernandes, meu conselheiro e maior motivador, que suporta as recaídas e festeja as vitórias ao meu lado. Além disso, me presenteou com nosso bem mais valioso, meu bebê Guilherme, que se tornou minha maior inspiração. Ao Papai Saulo e Mamãe Rosana obrigada por sempre estarem presentes me apoiando, auxiliando nos momentos difíceis, incentivando quando faltam forças e sendo exemplos a quem procuro-me espelhar todos os dias. Ao meu querido irmão Allan Saul que me surpreende todos os dias pela sua resiliência e que me cativa com toda sua intensidade. Me proporcionou tanta alegria com o amor de titia dinda da nossa linda Emanuella e novamente me agrada com a oportunidade de fazer parte de sua família e conviver com minha cunhada Mariana e meu sobrinho de coração Davi. A minha mais nova família meus sogros Lauriana e Carlos Alberto e meus cunhados Vítor, Laura e Vívian obrigada por me receberem tão bem. Sou muito grata por toda atenção e carinho e estou muito feliz por compartilhar minha vida com mais vocês. A todos colegas e amigos, em especial, Briana, Daniela, Cristina e Lorena pela agradável convivência diária na Embrapa e UnB e pelos valiosos momentos de diversão.

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Genome Sequence and Analysis of Buzura suppressaria Nucleopolyhedrovirus: A Group II Alphabaculovirus

Cited by 24 publications

References 58 publications

Genome Characteristics of the Cyclophragma Undans Nucleopolyhedrovirus: A Distinct Species in Group I of Alphabaculovirus

Genome Characteristics of the Cyclophragma Undans Nucleopolyhedrovirus: A Distinct Species in Group I of Alphabaculovirus

Protein composition of the occlusion bodies of Epinotia aporema granulovirus

Genoma e diversidade genética de populações de Chrysodeixis includens nucleopolyhedrovirus (ChinNPV)

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