Dosage spectrophotométrique du magnésium

Rusconi, Y.; Monnier, D.; Wenger, Paul Eugène Etienne

doi:10.1002/hlca.19480310615

Cited by 9 publications

(3 citation statements)

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“…Spermine tetrahydrochloride, spermidine trihydrochloride, and putrescine dihydrochloride were purchased from Sigma Chemical Co. Measurement of possible Mg2+ contamination of polyamines by the method of Rusconi et al (15) showed that these polyamines contained less than 0.001% Mg2+.…”

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confidence: 99%

Characterization of Aminoacyl Transfer Ribonucleic Acid Formation Stimulated by Polyamines

1972

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From a comparative study of aminoacyl transfer ribonucleic acid (tRNA) formation stimulated by polyamines or by Mg2+, it is shown that both reactions have the same requirements for adenosine triphosphate, tRNA, and aminoacyl-tRNA synthetase. Reaction kinetics are similar in both reactions. The same tRNA is aminoacylated in the presence of either spermine of Mg2+. Aminoacyl-tRNA formed in the presence of polyamines can be a donor of amino acids in polypeptide synthesis. The Mg2+ requirement of aminoacyl-transfer ribonucleic acid (tRNA) formation can be replaced by polyamines, such as spermine, spermidine, and putrescine (8 to 20). In the aminoacyl-tRNA formation stimulated by polyamines, we have found that amino acid-dependent PPI-adenosine triphosphate (ATP) exchange, formation of amino acid hydroxamate, and formation of aminoacyl-adenosine monophosphate (AMP)-enzyme complex do not occur, although aminoacyl-tRNA formation does occur (7, 7a, 22). These findings suggest that polyamines cannot satisfy the Mg2+ requirement of the first reaction of aminoacylation: amino acid + ATP + enzyme aminoacyl-AMP-enzyme + PP,; but can satisfy the second reaction: aminoacyl-AMP-enzyme + tRNA = aminoacyl-tRNA + AMP + enzyme. If polyamines are ineffective in the first reaction, the question arises as to whether aminoacyl-tRNA formed in the presence of polyamines is the same as that formed in the presence of Mg2+. The present study was carried out to answer this question. MATERIALS AND METHODS Preparation and purification of aminoacyl-tRNA synthetase. Aminoacyl-tRNA synthetase (EC 6.1.1) was prepared as described previously (20). The enzyme was purified further by hydroxylapatite and diethylaminoethyl (DEAE)-Sephadex A-50 column chromatography. The hydroxylapatite IPresent address: Faculty of Pharmaceutical Sciences,

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confidence: 99%

Characterization of Aminoacyl Transfer Ribonucleic Acid Formation Stimulated by Polyamines

1972

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“…Although the type material of Toretocnemus and Merriamia represents small individuals (fore fin lengths of approximately 5 cm and 12 cm, respectively), Delphinosaurus appears to be moderate in size (fore fin length in excess of 16 cm), and Shastasaurus (like Cymbospondylus) is extremely large (fore fin length in excess of 40 cm Baptanodon, but further research into the group may well demonstrate the inappropriateness of this allocation. The species described by Rusconi (1948) from the Jurassic of Argentina, Ancanamunia mendozana is based upon an incomplete skeleton, but the humerus is well preserved (Rusconi, 1948, fig. 65) and has three distal facets as in Ophthalmosaurus (Andrews, 1910, fig.…”

Section: Introductionmentioning

confidence: 99%

“…36). Furthermore, the single phalanx figured (Rusconi, 1948, fig. 69) has a rounded outline, like that of Ophthalmosaurus, and it seems certain that Rusconi's species should be referred to this genus.…”

Section: Introductionmentioning

confidence: 99%

Evolutionary trends in longipinnate ichthyosaurs, with particular reference to the skull and fore fin /

Macgowan¹

1972

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Suggested citation: Life Sci. Contr., R. Ont. Mus. ROYAL ONTARIO MUSEUM PUBLICATIONS IN LIFE SCIENCES The Royal Ontario Museum publishes three series in the Life Sciences: life sciences contributions, a numbered series of original scientific publications, including monographic works. life sciences occasional papers, a numbered series of original scientific publications, primarily short and usually of taxonomic significance. life sciences miscellaneous publications, an unnumbered series of publications of varied subject matter and format. All manuscripts considered for publication are subject to the scrutiny and editorial policies of the Life Sciences Editorial Board, and to review by persons outside the Museum staff who are authorities in the particular field involved.

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