Dosage physico-chimique de la nicotine

Direct Determination of Nicotine in Antismoking Pharmaceutical Products and in Tobacco Using an Inhibition Biosensor

“…The current is related to the hydrogen peroxide, which is oxidised at the platinum anode (see reaction 3), and, through the enzymatic reactions (1) and (2), to the butyrylcholine concentration and of course to the activity of the two enzymes; if butyrylcholine concentration is optimised and maintained constant, the current is related only to the activity of the enzymes, so that it is possible to determine the concentration of the inhibitor (nicotine) which can lower the activity of the enzymatic system (butyrylcholinesterase).…”

“…Many new test methods have been proposed for nicotine determination in the past few years(1) (titrimetric, spectrophotometric, chromatographic (2), and electrochemical -polarographic (3)(4), amperometric (5) or potentiometric) (6)(7)(8)(9). However, most of these methods were developed to determine nicotine in tobacco and not all are practicable for determinations in other real matrices.…”