Dosage of hemoglobin A1c by isoelectrofocusing

Schoos, Roland; Schoos-Barbette, S.; Lambotte, C

doi:10.1016/0009-8981(78)90458-8

Cited by 30 publications

(7 citation statements)

References 9 publications

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“…In the case of HbA Ia _ c the attachment of glucose to the amino groups of valine of the /J-chains alters the charge of the molecules in such a way that they can be easily separated by ion exchange chromatography or by isoelectric focusing (34). Although very useful for routine work these methods cannot be considered sufficiently specific; for instance, on column chromatography HbF emerges at the same position as HbAi c (35).…”

Section: Discussionmentioning

confidence: 99%

Specific Quantitation by HPLC of Protein (Lysine) Bound Glucose in Human Serum Albumin and Other Glycosylated Proteins

Schleicher¹,

Wieland²

1981

CCLM

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Summary: A specific and sensitive method for quantification of the fructose-lysine linkages present in nonenzymatically glycosylated albumin and other proteins is described. Protein is hydrolyzed for 18 h in 6 mol/1 HC1 at 95 °C to yield furosine (e-N-(2-furoylmethyl)-I-lysine) known as a specific degradation product of fructose-lysine. Furosine is then separated on HPLC and quantified by its UV-absorbance against a prepared fructose-lysine standard. The method has been successfully used for the determination of glycosyl-albumin in diabetic patients starting from 100 serum or less, as well as for various other proteins. Unlike the usually employed thiobarbituric acid assay the present procedure is truly specific for the detection of ketoamine linkages of glycosylated proteins. Spezifische Bestimmung von Protein (Lysin)-gebundener Glucose in Serumalbumin des Menschen und anderen glycosylierten Proteinen durch HochdruckflüssigchromatographieZusammenfassung: Es wird eine spezifische und empfindliche Methode zur Bestimmung der Fructose-Lysinbindungen von nichtenzymatisch glycosyliertem Albumin und anderen Proteinen beschrieben. Sie beruht auf der Hydrolyse des Proteins für 18 Stunden in 6 mol/1 HC1 bei 95 °C, wobei als spezifisches Abbauprodukt des Fructose-Lysins Furosin (e-N-(2-Furoylmethyl)-L-lysin) entsteht. Furosin wird dann durch HPLC abgetrennt und durch UV-Absorption gegen einen selbstsynthetisierten Fructose-Lysinstandard gemessen. Die Methode eignet sich fur die Bestimmung von glycosyliertem Albumin bei Diabetikern, ausgehend von 100 Serum und weniger, sowie auch allgemein für das Studium glycosylierter Proteine. Sie ist spezifischer als der häufig gebrauchte Thiobarbitursäure-Test, indem sie ausschließlich die Ketoaminbindungen glycosylierter Proteine erfaßt.

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Section: Discussionmentioning

confidence: 99%

Specific Quantitation by HPLC of Protein (Lysine) Bound Glucose in Human Serum Albumin and Other Glycosylated Proteins

Schleicher¹,

Wieland²

1981

CCLM

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“…All samples obtained during both experiments in each subject were assayed within the same series to eliminate interassay variations. HbA 1c levels were measured with an isofocusing method (21).…”

Section: A) Scheen and Associatesmentioning

confidence: 99%

Improvement of Insulin-Induced Glucose Disposal in Obese Patients With NIDDM After 1-Wk Treatment With d-Fenfluramine

Scheen¹,

Paolisso²,

Salvatore³

et al. 1991

Diabetes Care

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“…Using this assay, the minimal detection value was 0.01 pmol/ml and the interassay variation coefficient 6.3%. HbA~o was evaluated using isoelectric focussing [10]. A full blood count was performed with an automated counter.…”

Section: Laboratory Investigationsmentioning

confidence: 99%

Improvement of metabolic control in insulin dependent diabetics treated with the ?-glucosidase inhibitor Acarbose for two months

1981

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Summary. Acarbose, an a-glucosidase inhibitor, delays starch digestion and inhibits intestinal sucrase and maltase activity. Twenty-eight insulin dependent diabetics were given Acarbose (3 x 100 mg daily) over a two month period, preceded and followed by a two month placebo period. Acarbose reduced post-breakfast and post-dinner blood glucose values by 25% (p < 0.001) and 24% (p < 0.05) respectively. It also significantly reduced mean daily blood glucose by 18% (p < 0.05) and mean amplitude of glycaemic excursions from 8.0 _+ 0.6 to 5.5 + 0.4 mmol/1 (p <0.0005). Weight did not change significantly. Daily caloric and carbohydrate intake remained constant throughout the study while insulin requirements decreased slightly but significantly. Out of the 28 patients, 18 had absent while ten had slight residual B cell function as assessed by plasma C-peptide measurements. Treatment with Acarbose did not significantly affect residual B cell function. The beneficial effect of Acarbose on blood glucose control was seen in patients both with and without residual B cell secretion. The major side-effect was flatulence which was never severe enough to interrupt treatment, but led to a 50% reduction of the dose in one patient. It is concluded that Acarbose represents a useful additional means of improving metabolic control in insulin dependent diabetics.

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Dosage of hemoglobin A1c by isoelectrofocusing

Cited by 30 publications

References 9 publications

Specific Quantitation by HPLC of Protein (Lysine) Bound Glucose in Human Serum Albumin and Other Glycosylated Proteins

Specific Quantitation by HPLC of Protein (Lysine) Bound Glucose in Human Serum Albumin and Other Glycosylated Proteins

Improvement of Insulin-Induced Glucose Disposal in Obese Patients With NIDDM After 1-Wk Treatment With d-Fenfluramine

Improvement of metabolic control in insulin dependent diabetics treated with the ?-glucosidase inhibitor Acarbose for two months

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